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In this study, we developed a novel ciprofloxacin-functionalized silver nanocomposite (NC) by modifying phospholipid\u2013PEG (polyethylene glycol)-coated silver nanoparticles to enhance antibacterial efficacy against colistin-resistant P. aeruginosa. The NC was comprehensively characterized by UV-Visible spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy, transmission electron microscope, scanning electron microscopy, and EDX, confirming spherical morphology with an average size of 29.74\u00a0nm. Biological evaluations revealed potent antibacterial and antibiofilm activity: CFU (colony forming unit) and crystal violet assays demonstrated a 6.9 log\u2081\u2080 CFU mL\u22121 reduction in bacterial colonies and a 31.45% decrease in biofilm formation. Mechanistic studies revealed an elevated production of reactive oxygen species, singlet oxygen, and hydroxyl radicals upon treatment, correlating with a 39.70% decline in bacterial viability in XTT assays. Importantly, methyl thiazolyl tetrazolium cytotoxicity tests against MLE12 cells confirmed the NC\u2019s biocompatibility. Efficacy was further validated in an in vivo lung infection model in albino Wistar rats, where histopathological analysis and cytokine profiling confirmed therapeutic potential. Collectively, these findings establish ciprofloxacin-modified silver NCs as a stable, safe, and highly effective strategy for treating multidrug-resistant P. aeruginosa, offering a promising alternative to colistin in combating life-threatening lung infections.<\/jats:p>","DOI":"10.1093\/lambio\/ovag027","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T11:18:22Z","timestamp":1775474302000},"source":"Crossref","is-referenced-by-count":0,"title":["Ciprofloxacin-functionalized silver nanocomposite: a promising strategy against colistin-resistant\n                    <b>\n                      <i>Pseudomonas aeruginosa<\/i>\n                    <\/b>"],"prefix":"10.1093","volume":"79","author":[{"given":"Sameera","family":"Mujahid","sequence":"first","affiliation":[{"name":"Antimicrobial Resistance Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University , Aligarh 202002, Uttar Pradesh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samiya","family":"Farooq","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University , Aligarh 202002, Uttar Pradesh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Absar","family":"Talat","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University , Aligarh 202002, Uttar Pradesh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jawed","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Ramalingaswami Fellow, Multidisciplinary Centre for Advanced Research and Studies (MCARS) , Jamia Millia Islamia, New Delhi 110025 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kafil","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Aligarh Muslim University Pathology Department, Jawaharlal Nehru Medical College, , Aligarh 202002, Uttar Pradesh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9191-9423","authenticated-orcid":false,"given":"Asad U","family":"Khan","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University , Aligarh 202002, Uttar Pradesh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"2026051303063882100_bib1","first-page":"95","article-title":"Green synthesis of silver nanoparticles from natural compounds: glucose, eugenol and thymol","volume":"60","author":"Abed","year":"2019","journal-title":"J Adv Res Fluid Mech Therm Sci"},{"key":"2026051303063882100_bib2","doi-asserted-by":"publisher","first-page":"22","DOI":"10.3892\/wasj.2024.237","article-title":"Biological synthesis of silver nanoparticles and their medical applications (review)","volume":"6","author":"Ahmad","year":"2024","journal-title":"World Acad Sci J"},{"key":"2026051303063882100_bib3","doi-asserted-by":"publisher","first-page":"24464","DOI":"10.1038\/s41598-021-04008-x","article-title":"A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer","volume":"11","author":"Akhtar","year":"2021","journal-title":"Sci Rep"},{"key":"2026051303063882100_bib4","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.1002203","article-title":"Green synthesis of nanoparticles: a biological approach. 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