{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:33:19Z","timestamp":1786120399017,"version":"build-2736575974"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["215146 PDCPN2013-01-CONACYT"],"award-info":[{"award-number":["215146 PDCPN2013-01-CONACYT"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005739","name":"UNAM","doi-asserted-by":"publisher","award":["455026"],"award-info":[{"award-number":["455026"]}],"id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quinolone-resistant Escherichia coli are increasing in humans and livestock; however, their distribution and underlying mechanisms in shared environments remain incompletely characterized. We analyzed quinolone resistance in E. coli isolates from swine (n\u00a0=\u00a0100) and humans (n =\u00a065) associated with an agroecological farm in Morelos, Mexico. Antimicrobial susceptibility testing was performed for quinolones [nalidixic acid (NAL) and ciprofloxacin (CIP)], and the minimum inhibitory concentrations were determined. Phylogenetic grouping and genotypic analyses targeting gyrA mutations and plasmid-mediated quinolone resistance (PMQR) genes were conducted. High frequencies of\u00a0NAL and moderate CIP resistance were observed in both swine (NAL, 79%; CIP, 23%) and human (NAL, 65%; CIP, 15%) isolates. The predominant phenotype was NAL-resistant\/CIP-susceptible, particularly among commensals of phylogroups A and B1. High-level resistance to both antimicrobials was associated with double mutations in gyrA (S83L + D87N), whereas PMQR determinants, including qnr variants and aac(6\u2032)-Ib-cr, were widely distributed and linked to lower-level resistance. These findings indicate that quinolone resistance is shaped by the combined contribution of chromosomal mutations and plasmid-mediated mechanisms. The presence of PMQR determinants in isolates with reduced susceptibility highlights their potential role in the early stages of resistance development, emphasizing the urgent need for integrated surveillance of antimicrobial resistance in agricultural and community settings.<\/jats:p>","DOI":"10.1093\/lambio\/ovag062","type":"journal-article","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T11:39:07Z","timestamp":1784374747000},"source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic and genetic determinants of quinolone resistance in\n                    <i>Escherichia coli<\/i>\n                    at the human\u2013swine interface of an agroecological farm"],"prefix":"10.1093","volume":"79","author":[{"given":"Guillermina","family":"S\u00e1nchez-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa I","family":"Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Ciencias Gen\u00f3micas, UNAM , Cuernavaca, Morelos, CP 62210 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Tamayo-Legorreta","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Alpuche-Aranda","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto","family":"Barrios-Camacho","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulises","family":"Garza-Ramos","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Silva-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Grupo de Investigaci\u00f3n y Docencia en Resistencia Antimicrobiana (GID-RAM) , Cuernavaca, Morelos, CP 62100 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Centro de Ciencias Gen\u00f3micas, UNAM , Cuernavaca, Morelos, CP 62210 ,","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"2026080617065937300_bib1","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1021\/bi5000564","article-title":"Mechanism of quinolone action and 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