{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:32:56Z","timestamp":1787671976480,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2022-NR070407"],"award-info":[{"award-number":["RS-2022-NR070407"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Slow-transit constipation (STC) is characterized by reduced colonic motility, impaired smooth muscle function, and altered mucosal signaling. Among these pathways, mucosal serotonin (5-HT), mainly produced by enterochromaffin cells, contributes to intestinal motility regulation. In a loperamide-induced constipation model in male C57BL\/6\u00a0J mice, we evaluated the effects of Lactiplantibacillus plantarum NCHBL-004 on bowel function and associated molecular changes. Compared with loperamide-treated mice, live NCHBL-004 at 1\u00a0\u00d7\u00a010\u2079 CFU improved fecal output and gastrointestinal transit and partially preserved fecal water content, whereas the lower live dose and heat-killed preparation showed no significant effects. These functional changes were accompanied by preservation of colonic muscularis thickness and increased expression of smooth muscle-associated markers, including Acta2 and Myh11. NCHBL-004 also modulated mucosal serotonergic gene expression, as indicated by increased Tph1, which encodes a key enzyme for 5-HT synthesis, and decreased Slc6a4, which encodes the serotonin transporter involved in 5-HT reuptake. Overall, these findings suggest that live NCHBL-004 improves intestinal motility in loperamide-induced constipation, with associated changes in colonic smooth muscle phenotype and mucosal serotonergic regulation.<\/jats:p>","DOI":"10.1093\/lambio\/ovag063","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T11:32:46Z","timestamp":1784633566000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Lactiplantibacillus plantarum<\/i>\n                    NCHBL-004 improves intestinal motility and modulates serotonergic gene expression in a loperamide-induced constipation model"],"prefix":"10.1093","volume":"79","author":[{"given":"Wan-Gyu","family":"Kim","sequence":"first","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Sung","family":"Lee","sequence":"additional","affiliation":[{"name":"Nodcure, Inc. , 77 Yongbong-ro, Buk-gu, Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeong-Jun","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-Su","family":"Seo","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Yeon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-yeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Eun","family":"Jung","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geun-Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Jung","family":"Song","sequence":"additional","affiliation":[{"name":"Nodcure, Inc. , 77 Yongbong-ro, Buk-gu, Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ah-Ra","family":"Jang","sequence":"additional","affiliation":[{"name":"Nodcure, Inc. , 77 Yongbong-ro, Buk-gu, Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Bum","family":"Koh","sequence":"additional","affiliation":[{"name":"Nodcure, Inc. , 77 Yongbong-ro, Buk-gu, Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4664-5640","authenticated-orcid":false,"given":"Jong-Hwan","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University , Gwangju 61186 ,","place":["Republic of Korea"]},{"name":"Nodcure, Inc. , 77 Yongbong-ro, Buk-gu, Gwangju 61186 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"2026082511150955700_bib1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1136\/gut.2005.073197","article-title":"The role of glial cells and apoptosis of enteric neurones in the neuropathology of intractable slow transit constipation","volume":"55","author":"Bassotti","year":"2005","journal-title":"Gut"},{"key":"2026082511150955700_bib2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S1569-7339(10)70079-5","article-title":"The degradation of serotonin: role of MAO","volume":"21","author":"Bortolato","year":"2010","journal-title":"Handbook Behav Neurosci"},{"key":"2026082511150955700_bib3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145153","article-title":"Smooth muscle-like cells generated from human mesenchymal stromal cells display marker gene expression and electrophysiological competence comparable to bladder smooth muscle cells","volume":"10","author":"Brun","year":"2015","journal-title":"PLoS One"},{"key":"2026082511150955700_bib4","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.899069","article-title":"The serotonergic system dysfunction in diabetes mellitus","volume":"16","author":"Cai","year":"2022","journal-title":"Front Cell Neurosci"},{"key":"2026082511150955700_bib5","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1007\/s12602-021-09814-3","article-title":"Lactobacillus plantarum PS128 promotes intestinal motility, mucin production, and serotonin signaling in mice","volume":"14","author":"Chen","year":"2021","journal-title":"Probio Antimicro Prot"},{"key":"2026082511150955700_bib6","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.pharmthera.2011.03.013","article-title":"Ontogeny and regulation of the serotonin transporter: providing insights into human disorders","volume":"131","author":"Daws","year":"2011","journal-title":"Pharmacol Ther"},{"key":"2026082511150955700_bib7","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1111\/j.1365-2982.2009.01401.x","article-title":"Pathophysiology of colonic causes of chronic constipation","volume":"21","author":"Dinning","year":"2009","journal-title":"Neurogastroenterol Motil"},{"key":"2026082511150955700_bib8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2007.00970.x","article-title":"Recent advances in understanding the role of serotonin in gastrointestinal motility and functional bowel disorders","volume":"19","author":"Galligan","year":"2007","journal-title":"Neurogastroenterol Motil"},{"key":"2026082511150955700_bib9","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1097\/MED.0b013e32835bc703","article-title":"5-Hydroxytryptamine (serotonin) in the gastrointestinal tract","volume":"20","author":"Gershon","year":"2013","journal-title":"Curr Opin Endocrinol Diabet Obes"},{"key":"2026082511150955700_bib10","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.4314\/tjpr.v18i6.8","article-title":"Effect of atractylenolide III on interstitial cells of Cajal and C-kit\/SCF pathway of rats with loperamide-induced slow transit constipation","volume":"18","author":"Hao","year":"2021","journal-title":"Trop J Pharm Res"},{"key":"2026082511150955700_bib11","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1206147","article-title":"Evolution and function of calponin and transgelin","volume":"11","author":"Hsieh","year":"2023","journal-title":"Front Cell Dev Biol"},{"key":"2026082511150955700_bib12","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1035538","article-title":"Connecting gut microbiomes and short chain fatty acids with the serotonergic system and behavior in Gallus gallus and other avian species","volume":"13","author":"Jadhav","year":"2022","journal-title":"Front Physiol"},{"key":"2026082511150955700_bib13","doi-asserted-by":"publisher","first-page":"112565","DOI":"10.1016\/j.nut.2024.112565","article-title":"Lactobacillus plantarum NCHBL-004 modulates high-fat diet-induced weight gain and enhances GLP-1 production for blood glucose regulation","volume":"128","author":"Jang","year":"2024","journal-title":"Nutrition"},{"key":"2026082511150955700_bib14","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1174968","article-title":"Multi-strain probiotics alleviate loperamide-induced constipation by adjusting the microbiome, serotonin, and short-chain fatty acids in rats","volume":"14","author":"Jeong","year":"2023","journal-title":"Front Microbiol"},{"key":"2026082511150955700_bib15","article-title":"Laxative effect of spicatoside a by cholinergic regulation of enteric nerve in loperamide-induced constipation: ICR mice model","volume":"24","author":"Kim","year":"2019","journal-title":"Molecules"},{"key":"2026082511150955700_bib16","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-017-0576-y","article-title":"Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion","volume":"17","author":"Kim","year":"2017","journal-title":"BMC Gastroenterol"},{"key":"2026082511150955700_bib17","doi-asserted-by":"publisher","first-page":"35","DOI":"10.5625\/lar.2014.30.1.35","article-title":"Metabolomics approach to serum biomarker for loperamide-induced constipation in SD rats","volume":"30","author":"Kim","year":"2014","journal-title":"Lab Anim Res"},{"key":"2026082511150955700_bib18","doi-asserted-by":"publisher","DOI":"10.3390\/nu12010225","article-title":"Lactobacillus reuteri DSM 17938 and magnesium oxide in children with functional chronic constipation: a double-blind and randomized clinical trial","volume":"12","author":"Kubota","year":"2020","journal-title":"Nutrients"},{"key":"2026082511150955700_bib19","doi-asserted-by":"publisher","first-page":"8998","DOI":"10.1523\/JNEUROSCI.6684-10.2011","article-title":"Essential roles of enteric neuronal serotonin in gastrointestinal motility and the development\/survival of enteric dopaminergic neurons","volume":"31","author":"Li","year":"2011","journal-title":"J Neurosci"},{"key":"2026082511150955700_bib20","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1689597","article-title":"Regulatory mechanisms of the gut microbiota-short chain fatty acids signaling axis in slow transit constipation and progress in multi-target interventions","volume":"16","author":"Liu","year":"2025","journal-title":"Front Microbiol"},{"key":"2026082511150955700_bib21","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1254\/jjp.90.28","article-title":"In vivo assessment of acceleration of motor activity associated with acetylcholine release via 5-hydroxytryptamine4 receptor in dog intestine","volume":"90","author":"Makimoto","year":"2002","journal-title":"Jpn J Pharmacol"},{"key":"2026082511150955700_bib22","doi-asserted-by":"publisher","first-page":"5847","DOI":"10.1016\/j.csbj.2022.10.026","article-title":"Integrated gut microbiome and metabolome analyses identified fecal biomarkers for bowel movement regulation by Bifidobacterium longum BB536 supplementation: a RCT","volume":"20","author":"Nakamura","year":"2022","journal-title":"Comput Struct Biotechnol J"},{"key":"2026082511150955700_bib23","doi-asserted-by":"publisher","DOI":"10.3390\/nu14183704","article-title":"Crosstalk between the gut microbiome and colonic motility in chronic constipation: potential mechanisms and microbiota modulation","volume":"14","author":"Pan","year":"2022","journal-title":"Nutrients"},{"key":"2026082511150955700_bib24","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1097\/JXX.0000000000000760","article-title":"Evidence-based treatment recommendations for OTC management of chronic constipation","volume":"34","author":"Rao","year":"2022","journal-title":"J Am Assoc Nurse Pract"},{"key":"2026082511150955700_bib25","doi-asserted-by":"publisher","first-page":"11302","DOI":"10.1063\/5.0176784","article-title":"Mechanoregulation and function of calponin and transgelin","volume":"5","author":"Rasmussen","year":"2024","journal-title":"Biophysics Rev"},{"key":"2026082511150955700_bib26","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1016\/j.jcmgh.2021.05.008","article-title":"Serotonin as a mitogen in the gastrointestinal tract: revisiting a familiar molecule in a new role","volume":"12","author":"Shah","year":"2021","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"2026082511150955700_bib27","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgast-2019-000322","article-title":"Associations of chronic diarrhoea with non-alcoholic fatty liver disease and obesity-related disorders among US adults","volume":"6","author":"Shin","year":"2019","journal-title":"BMJ Open Gastroenterol"},{"key":"2026082511150955700_bib28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3920\/BM2016.0158","article-title":"Differences between live and heat-killed bifidobacteria in the regulation of immune function and the intestinal environment","volume":"8","author":"Sugahara","year":"2017","journal-title":"BM"},{"key":"2026082511150955700_bib29","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.890314","article-title":"Bifidobacterium lactis TY-S01 prevents loperamide-induced constipation by modulating gut microbiota and its metabolites in mice","volume":"9","author":"Tang","year":"2022","journal-title":"Front Nutr"},{"key":"2026082511150955700_bib30","first-page":"60","article-title":"Idiopathic slow transit constipation: pathophysiology, diagnosis, and management","author":"Vlismas","year":"2024","journal-title":"Medicina"},{"key":"2026082511150955700_bib31","first-page":"2021","article-title":"Roles of gut microbiota and metabolites in pathogenesis of functional constipation","author":"Wang","year":"2021","journal-title":"Evid Based Complement Alternat Med eCAM"},{"key":"2026082511150955700_bib32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2006.00781.x","article-title":"Novel smooth muscle markers reveal abnormalities of the intestinal musculature in severe colorectal motility disorders","volume":"18","author":"Wedel","year":"2006","journal-title":"Neurogastroenterol Motil"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovag063\/69685151\/ovag063.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/8\/ovag063\/69685151\/ovag063.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/8\/ovag063\/69685151\/ovag063.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:15:12Z","timestamp":1787670912000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovag063\/8739104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,3]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovag063","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,7,21]]},"article-number":"ovag063"}}