{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:02:01Z","timestamp":1790614921561,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100005283","name":"Cearense Foundation for the Support of Scientific and Technological Development","doi-asserted-by":"publisher","award":["BP6-0241-00292.01.00\/25"],"award-info":[{"award-number":["BP6-0241-00292.01.00\/25"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to identify kefir-derived microorganisms, evaluate their probiotic potential to select the most promising strain, and assess the selected strain\u2019s antibacterial and antibiofilm activities against foodborne isolates, Escherichia coli, and Klebsiella pneumoniae. Kefir grains were cultivated in selective media, and isolates were identified using MALDI-TOF mass spectrometry. Their acid and NaCl tolerance, auto- and co-aggregation, and antimicrobial activity were assessed to identify the most effective probiotic strain. Subsequently, the selected strain was subjected to spot overlay tests, broth microdilution assays, and biofilm inhibition experiments against E. coli and K. pneumoniae isolates. Enterococcus durans, Leuconostoc pseudomesenteroides, Lacticaseibacillus paracasei, and Kluyveromyces marxianus were identified. Among these, L. paracasei (Lpk-01) demonstrated the best probiotic characteristics, with 50% survival at pH 5, tolerance to 2% NaCl, and high co-aggregation with K. pneumoniae (40%). It inhibited pathogens (inhibition zones &amp;gt; 14 mm), and its cell-free supernatant (Lpk-cf) reduced biofilm biomass by \u223c50%, with effects minimized upon neutralization, suggesting the involvement of organic acids. These findings affirm the antimicrobial potential of L. paracasei, highlighting its application as a natural alternative against bacterial pathogens.<\/jats:p>","DOI":"10.1093\/lambio\/ovag069","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:48:56Z","timestamp":1786535336000},"source":"Crossref","is-referenced-by-count":0,"title":["Kefir-derived\n                    <i>Lacticaseibacillus paracasei<\/i>\n                    as a promising candidate against planktonic and biofilm cells of foodborne\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1093","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0116-0957","authenticated-orcid":false,"given":"Victor Alves","family":"Carneiro","sequence":"first","affiliation":[{"name":"Center for Bioprospecting and Applied Molecular Experimentation (NUBEM\/LaBAM), University Center INTA \u2013 UNINTA , Sobral, Cear\u00e1 60.192-010 ,","place":["Brazil"]},{"name":"Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Faculty of Medicine, Federal University of Cear\u00e1 \u2013 UFC , Sobral, Cear\u00e1 62.048-280 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Leilah Monte Coelho","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Center for Bioprospecting and Applied Molecular Experimentation (NUBEM\/LaBAM), University Center INTA \u2013 UNINTA , Sobral, Cear\u00e1 60.192-010 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edrine Vasconcelos Farias","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Faculty of Medicine, Federal University of Cear\u00e1 \u2013 UFC , Sobral, Cear\u00e1 62.048-280 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Adenes Teixeira","family":"Coelho","sequence":"additional","affiliation":[{"name":"Center for Bioprospecting and Applied Molecular Experimentation (NUBEM\/LaBAM), University Center INTA \u2013 UNINTA , Sobral, Cear\u00e1 60.192-010 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benise Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Center for Bioprospecting and Applied Molecular Experimentation (NUBEM\/LaBAM), University Center INTA \u2013 UNINTA , Sobral, Cear\u00e1 60.192-010 ,","place":["Brazil"]},{"name":"Northeast Network of Biotechnology Program (RENORBIO), State University of Cear\u00e1, Campus Itaperi , Fortaleza, Cear\u00e1 60.714-903 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Mesquita Bastos","family":"Cavalcante","sequence":"additional","affiliation":[{"name":"Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Faculty of Medicine, Federal University of Cear\u00e1 \u2013 UFC , Sobral, Cear\u00e1 62.048-280 ,","place":["Brazil"]},{"name":"Northeast Network of Biotechnology Program (RENORBIO), State University of Cear\u00e1, Campus Itaperi , Fortaleza, Cear\u00e1 60.714-903 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Albuquerque","family":"Costa","sequence":"additional","affiliation":[{"name":"Center for Bioprospecting and Applied Molecular Experimentation (NUBEM\/LaBAM), University Center INTA \u2013 UNINTA , Sobral, Cear\u00e1 60.192-010 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026092812033713300_bib1","doi-asserted-by":"publisher","first-page":"116676","DOI":"10.1016\/j.lwt.2024.116676","article-title":"Evaluation of the probiotic, antibacterial, antibiofilm, and safety properties of Lacticaseibacillus paracasei B31\u20132","volume":"207","author":"Behbahani","year":"2024","journal-title":"LWT"},{"key":"2026092812033713300_bib2","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1016\/j.foodres.2017.09.093","article-title":"Simulated gastrointestinal conditions increase adhesion ability of Lactobacillus paracasei strains isolated from kefir to Caco-2 cells and mucin","volume":"103","author":"Bengoa","year":"2018","journal-title":"Food Res Int"},{"key":"2026092812033713300_bib3","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1111\/1471-0307.12759","article-title":"Microbial community dynamics of fermented kefir beverages changes over time","volume-title":"Int J Dairy Tech","author":"Brasiel","year":"2021"},{"key":"2026092812033713300_bib4","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s13099-017-0162-4","article-title":"Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion","volume":"9","author":"Campana","year":"2017","journal-title":"Gut Pathog"},{"key":"2026092812033713300_bib5","doi-asserted-by":"publisher","first-page":"1578","DOI":"10.3389\/fmicb.2020.01578","article-title":"Isolation of a Leuconostoc mesenteroides strain with anti-porcine epidemic diarrhea virus activities from kefir grains","volume":"11","author":"Chang-Liao","year":"2020","journal-title":"Front Microbiol"},{"key":"2026092812033713300_bib6","doi-asserted-by":"publisher","first-page":"789","DOI":"10.3389\/fmicb.2019.00789","article-title":"Antimicrobial activity of Lactobacillus species against carbapenem-resistant Enterobacteriaceae","volume":"10","author":"Chen","year":"2019","journal-title":"Front Microbiol"},{"key":"2026092812033713300_bib7","doi-asserted-by":"publisher","first-page":"e70","DOI":"10.1590\/S1678-9946201759070","article-title":"Drug resistance, AmpC-\u03b2-lactamase and extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae isolated from fish and shrimp","volume":"59","author":"de\u00a0Almeida","year":"2017","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"2026092812033713300_bib8","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1046\/j.1365-2672.2000.00845.x","article-title":"Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum","volume":"31","author":"Del\u00a0Re","year":"2000","journal-title":"Lett Appl Microbiol"},{"key":"2026092812033713300_bib9","doi-asserted-by":"publisher","first-page":"1806","DOI":"10.3390\/nu11081806","article-title":"Fermented foods: definitions and characteristics, impact on the gut microbiota and effects on gastrointestinal health and disease","volume":"11","author":"Dimidi","year":"2019","journal-title":"Nutrients"},{"key":"2026092812033713300_bib10","doi-asserted-by":"publisher","first-page":"ovac006","DOI":"10.1093\/lambio\/ovac006","article-title":"Effect of Lactobacillus spp. cell-free supernatant against planktonic growth and biofilm formation of foodborne Escherichia coli isolates","volume":"76","author":"Fernandes","year":"2023","journal-title":"Lett Appl Microbiol"},{"key":"2026092812033713300_bib11","first-page":"221","article-title":"Antibiofilm activity of kefir probiotic lactobacilli against uropathogenic Escherichia coli (UPEC)","volume":"12","author":"Ghane","year":"2020","journal-title":"Avicenna J Med Biotechnol"},{"key":"2026092812033713300_bib12","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s12866-022-02751-3","article-title":"Antibacterial and antibiofilm potential of Lacticaseibacillus rhamnosus YT and its cell-surface extract","volume":"23","author":"Guan","year":"2023","journal-title":"BMC Microbiol"},{"key":"2026092812033713300_bib13","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3390\/biomedicines5020031","article-title":"Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria","volume":"5(2)","author":"Halder","year":"2017","journal-title":"Biomedicines"},{"key":"2026092812033713300_bib14","doi-asserted-by":"publisher","first-page":"3207","DOI":"10.1099\/00221287-133-11-3207","article-title":"A comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius","volume":"133","author":"Handley","year":"1987","journal-title":"Microbiology"},{"key":"2026092812033713300_bib15","doi-asserted-by":"publisher","first-page":"1997","DOI":"10.1002\/fsn3.1025","article-title":"Characterization of antimicrobial activity of three Lactobacillus plantarum strains isolated from Chinese traditional dairy food","volume":"7","author":"Hu","year":"2019","journal-title":"Food Sci Nutr"},{"key":"2026092812033713300_bib16","doi-asserted-by":"publisher","first-page":"5995","DOI":"10.1038\/s41598-023-33062-w","article-title":"Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen","volume":"13","author":"Keeratikunakorn","year":"2023","journal-title":"Sci Rep"},{"key":"2026092812033713300_bib17","doi-asserted-by":"publisher","first-page":"e12728","DOI":"10.1111\/jfs.12728","article-title":"Microbial composition of Korean kefir and antimicrobial activity of Acetobacter fabarum DH1801","volume":"40","author":"Kim","year":"2020","journal-title":"J Food Saf"},{"key":"2026092812033713300_bib18","doi-asserted-by":"publisher","first-page":"2667","DOI":"10.3390\/microorganisms13122667","article-title":"Lacticaseibacillus casei combats biofilm formation and exhibits antibacterial activity against clinical isolates of Staphylococcus aureus, Salmonella enterica, and Escherichia coli","volume":"13","author":"Kiousi","year":"2025","journal-title":"Microorganisms"},{"key":"2026092812033713300_bib19","doi-asserted-by":"publisher","first-page":"103994","DOI":"10.1016\/j.langlo.2026.103994","article-title":"WHO estimates of the global, regional, and national burden of 42 foodborne infectious and chemical hazards, 2000\u201321: an updated data synthesis","volume":"14","author":"Lake","year":"2026","journal-title":"Lancet Glob Health"},{"key":"2026092812033713300_bib20","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1007\/s10068-020-00837-0","article-title":"Antimicrobial and anti-biofilm effects of probiotic Lactobacillus plantarum KU200656 isolated from kimchi","volume":"30","author":"Lee","year":"2020","journal-title":"Food Sci Biotechnol"},{"key":"2026092812033713300_bib21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5851\/kosfa.2020.e75","article-title":"Etiological agents implicated in foodborne illness worldwide","volume":"41","author":"Lee","year":"2021","journal-title":"Food Sci Anim Resour"},{"key":"2026092812033713300_bib22","doi-asserted-by":"publisher","first-page":"112829","DOI":"10.1016\/j.ejmech.2020.112829","article-title":"\u03b2-lactam antibiotics: an overview from a medicinal chemistry perspective","volume":"208","author":"Lima","year":"2020","journal-title":"Eur J Med Chem"},{"key":"2026092812033713300_bib23","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.lwt.2019.05.072","article-title":"Characterization and application of postbiotics of Lactobacillus spp. on Listeria monocytogenes in vitro and food models","volume":"111","author":"Moradi","year":"2019","journal-title":"LWT"},{"key":"2026092812033713300_bib24","first-page":"123","article-title":"Screening and characterization of in-vitro probiotic criteria of Saccharomyces and Kluyveromyces strains","volume":"10","author":"Moradi","year":"2018","journal-title":"Iran J Microbiol"},{"key":"2026092812033713300_bib25","doi-asserted-by":"crossref","first-page":"426946","DOI":"10.3389\/fmicb.2019.00300","article-title":"Probiotic properties of enterococcus isolated from artisanal dairy products","volume":"10","author":"Nami","year":"2019","journal-title":"Front Microbiol"},{"key":"2026092812033713300_bib26","doi-asserted-by":"publisher","first-page":"e2437","DOI":"10.3791\/2437","article-title":"Microtiter dish biofilm formation assay","volume":"47","author":"O\u2019Toole","year":"2011","journal-title":"JoVE"},{"key":"2026092812033713300_bib27","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/978-3-319-99664-6","article-title":"Current trends in antimicrobial resistance of Escherichia coli","volume":"416","author":"Paitan","year":"2018","journal-title":"Curr Top Microbiol Immunol"},{"key":"2026092812033713300_bib28","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1007\/s00284-019-01842-1","article-title":"Shiga toxin-producing and enteroaggregative Escherichia coli in animal, foods, and humans: pathogenicity mechanisms, detection methods, and epidemiology","volume":"77","author":"Paletta","year":"2020","journal-title":"Curr Microbiol"},{"key":"2026092812033713300_bib29","doi-asserted-by":"publisher","first-page":"297","DOI":"10.3390\/microorganisms8020297","article-title":"Safety aspects of genetically modified lactic acid bacteria","volume":"8","author":"Plavec","year":"2020","journal-title":"Microorganisms"},{"key":"2026092812033713300_bib30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/biomedicines8120594","article-title":"Isolation of a Lactobacillus paracasei strain with probiotic attributes from kefir grains","volume":"8","author":"Plessas","year":"2020","journal-title":"Biomedicines"},{"key":"2026092812033713300_bib31","doi-asserted-by":"publisher","first-page":"2584","DOI":"10.1007\/s00284-020-02016-0","article-title":"Probiotic potential of Lactobacillus paracasei CT12 isolated from water kefir grains (Tibicos)","volume":"77","author":"Romero-Luna","year":"2020","journal-title":"Curr Microbiol"},{"key":"2026092812033713300_bib32","doi-asserted-by":"publisher","first-page":"110148","DOI":"10.1016\/j.ijfoodmicro.2023.110148","article-title":"The role of food chain in antimicrobial resistance spread and One Health approach to reduce risks","volume-title":"Int J Food Microbiol","author":"Sagar","year":"2023"},{"key":"2026092812033713300_bib33","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/s13205-016-0591-7","article-title":"Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens","volume-title":"3 Biotech","author":"Sharma","year":"2017"},{"key":"2026092812033713300_bib34","doi-asserted-by":"publisher","first-page":"1999","DOI":"10.1007\/s11274-010-0384-9","article-title":"Influence of cell surface properties on adhesion ability of bifidobacteria","volume":"26","author":"Wang","year":"2010","journal-title":"World J Microbiol Biotechnol"},{"key":"2026092812033713300_bib35","doi-asserted-by":"publisher","first-page":"1529","DOI":"10.2147\/IJGM.S496799","article-title":"Epidemiological characteristics of foodborne disease outbreaks in a hospital: a 5-year retrospective study","volume":"18","author":"Yang","year":"2025","journal-title":"Int J Gen Med"},{"key":"2026092812033713300_bib36","doi-asserted-by":"publisher","first-page":"3423","DOI":"10.3390\/ijms20143423","article-title":"Biofilms: the microbial \u201cprotective clothing\u201d in extreme environments","volume":"20","author":"Yin","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2026092812033713300_bib37","doi-asserted-by":"publisher","first-page":"888","DOI":"10.3390\/microorganisms14040888","article-title":"Antibiofilm potential and mechanisms of Lacticaseibacillus paracasei L475 against multidrug-resistant Escherichia coli isolated from older adults","volume":"14","author":"Zhang","year":"2026","journal-title":"Microorganisms"},{"key":"2026092812033713300_bib38","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1099\/ijsem.0.004107","article-title":"A taxonomic note on the genus Lactobacillus: description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae","volume":"70","author":"Zheng","year":"2020","journal-title":"Int J Syst Evol Microbiol"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovag069\/70634008\/ovag069.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/9\/ovag069\/70634008\/ovag069.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/9\/ovag069\/70634008\/ovag069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:07:26Z","timestamp":1790611646000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovag069\/8761028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,4]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovag069","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,13]]},"article-number":"ovag069"}}