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Higher reaction temperatures favor tagatose production, but microbial whole-cell conversion is limited by the viability of cells at elevated temperatures. This work attempts to address this by engineering\u00a0Lactiplantibacillus plantarum for growth at elevated temperatures towards development of a high-temperature galactose-to-tagatose isomerization biocatalytic system. Using adaptive laboratory evolution (ALE), a strain capable of growth at 43\u00b0C was isolated over 85 passages of increasing thermal stress. The adaptation required using a cycling approach, where cultures were cultured at permissive (37\u00b0C) temperatures between periods of growth at elevated temperatures. At passage #85, the cells were able to grow at 43\u00b0C over multiple passages, indicating complete adaptation. They were also able to grow well at 37\u00b0C. Unfortunately, the galactose-to-tagatose conversion reaction at 37, 50, and 60\u00b0C using an L-arabinose isomerase-transformed strain revealed no benefit of using the evolved strain over the parental strain. Nevertheless, the ALE protocol and isolated strain may be of benefit for other applications.<\/jats:p>","DOI":"10.1093\/lambio\/ovag084","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:18:32Z","timestamp":1790425112000},"source":"Crossref","is-referenced-by-count":0,"title":["Thermal adaptation of\n                    <i>Lactiplantibacillus plantarum<\/i>\n                    by laboratory evolution does not enhance tagatose biosynthesis"],"prefix":"10.1093","volume":"79","author":[{"given":"Vikas D","family":"Trivedi","sequence":"first","affiliation":[{"name":"Department of Chemical & Biological Engineering, Tufts University , Medford, MA 02155 ,\u00a0","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd C","family":"Chappell","sequence":"additional","affiliation":[{"name":"Department of Chemical & Biological Engineering, Tufts University , Medford, MA 02155 ,\u00a0","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7737-1385","authenticated-orcid":false,"given":"Nikhil U","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Chemical & Biological Engineering, Tufts University , Medford, MA 02155 ,\u00a0","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"2026102205285817700_bib1","doi-asserted-by":"publisher","first-page":"25287","DOI":"10.1073\/pnas.1909987116","article-title":"Adaptive evolution reveals a tradeoff between growth rate and oxidative stress during naphthoquinone-based aerobic respiration","volume":"116","author":"Anand","year":"2019","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026102205285817700_bib2","doi-asserted-by":"publisher","first-page":"4548","DOI":"10.1038\/s41467-019-12497-8","article-title":"Galactose to tagatose 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