{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:56:36Z","timestamp":1791471396564,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Antimicrobial resistance poses a major global health challenge, highlighting the need for alternative antimicrobial strategies. In this study, silver-containing colloidal aggregates were synthesized using an extract from Artocarpus heterophyllus leaves and evaluated for their antimicrobial activity and effects on microbial survival under adaptive conditions. Silver-containing colloidal aggregates were characterized for size, structure, and stability, followed by antimicrobial assessment using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal\/Fungicidal Concentration (MBC\/MFC) assays. The synthesized silver-containing colloidal aggregates exhibited an average size of 444.00 \u00b1 32.84 nm, a polydispersity index of 0.81 \u00b1 0.16, and a zeta potential of \u221224.28 \u00b1 10.66 mV. Functional group analysis indicated that phytochemicals mediated the reduction of Ag\u207a ions. Antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus, and Candida albicans, with MIC values of 80, 640, and 320 mg l\u22121, respectively, and MBC\/MFC values of 1280 mg l\u22121 (E. coli and C. albicans) and 5120 mg l\u22121 (S. aureus). Adaptive laboratory evolution (ALE) experiments under gradually increasing silver-containing colloidal particle concentrations showed a progressive decline in microbial growth, with no detectable survival at higher exposure levels. These findings suggest that silver-containing colloidal aggregates exhibit antimicrobial activity and may limit short-term microbial adaptation under the tested conditions.<\/jats:p>","DOI":"10.1093\/lambio\/ovag086","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:50:23Z","timestamp":1791460223000},"source":"Crossref","is-referenced-by-count":0,"title":["Assessing microbial resistance to\n                    <i>Artocarpus heterophyllus<\/i>\n                    leaf\u2013mediated AgNO3 colloidal aggregates using adaptive laboratory evolution"],"prefix":"10.1093","author":[{"given":"Maya","family":"Fitriyanti","sequence":"first","affiliation":[{"name":"School of Life Sciences and Technology, Bandung Institute of Technology , Ganesha 10, Bandung , 40132","place":["Indonesia"]},{"name":"Biosciences and Biotechnology Research Centre, Institut Teknologi Bandung , Jl. Ganesa 10 Bandung 40132 ,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almando","family":"Geraldi","sequence":"additional","affiliation":[{"name":"Faculty of Science and Technology Department of Biology, , Universitas Airlangga, Surabaya 60115 ,","place":["Indonesia"]},{"name":"University-Centre of Excellence-Research Centre for Bio-Molecule Engineering , Universitas Airlangga, Surabaya 60115 ,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rakhmawati","sequence":"additional","affiliation":[{"name":"Faculty of Mathematics and Natural Sciences Department of Biology Education, , Universitas Negeri Yogyakarta, Jl. Colombo 1, Karangmalang, Yogyakarta ,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Rosalinda","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Bandung Institute of Technology , Ganesha 10, Bandung , 40132","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najwa","family":"Fadhilatunnisa","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Bandung Institute of Technology , Ganesha 10, Bandung , 40132","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurhamidah Almatin","family":"Hidayat","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Bandung Institute of Technology , Ganesha 10, Bandung , 40132","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arienda Elita","family":"Amadea","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Bandung Institute of Technology , Ganesha 10, Bandung , 40132","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,8]]},"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovag086\/71718573\/ovag086.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovag086\/71718573\/ovag086.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:22:24Z","timestamp":1791469344000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article\/doi\/10.1093\/lambio\/ovag086\/8885586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,8]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovag086","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,8]]},"article-number":"ovag086"}}