{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:43:00Z","timestamp":1792662180241,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22508230"],"award-info":[{"award-number":["22508230"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sophorose is a strong soluble inducer of cellulase biosynthesis in Trichoderma reesei, but its practical use remains limited by availability and cost. In this study, an integrated strategy was developed to improve cellulase production by combining sophorose biosynthesis, sophorose-based induction, and heterologous \u03b2-glucosidase expression. \u03b2-glucosidases from T. reesei QM6a, Penicillium digitatum Pdw03, and Aspergillus niger CBS 513.88 were expressed in Escherichia coli BL21(DE3) and compared for sophorose formation. The A. niger-derived enzyme rAnCel1A showed the highest transglycosylation activity, producing 8.42\u00a0\u00b1\u00a00.21\u00a0g L\u22121 sophorose at 47\u00b0C and showing optimal performance at pH 3.2. In a 20-L bioreactor, an enzymatically synthesized sophorose\u2013glucose mixture containing 12.7\u00a0\u00b1\u00a00.8\u00a0g L\u22121 sophorose triggered detectable cellulase production within 24\u00a0h after induction, whereas glucose-only feeding failed to initiate cellulase production despite comparable biomass formation. Heterologous expression of fungal cel1A genes in T. reesei Rut-C30 further increased \u03b2-glucosidase and filter paper activities. The An-cel1A advantage disappeared after gene-dosage normalization, suggesting higher expression. These results provide a feasible framework for coupling inducer biosynthesis with cellulase production in T. reesei.<\/jats:p>","DOI":"10.1093\/lambio\/ovag088","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:53:29Z","timestamp":1791978809000},"source":"Crossref","is-referenced-by-count":0,"title":["Sophorose biosynthesis with heterologous beta-glucosidases for cellulase production in\n                    <i>Trichoderma reesei<\/i>"],"prefix":"10.1093","volume":"79","author":[{"given":"Jiaming","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"Zibo Key Laboratory of Synthetic Biology and New Pharmaceutical Applications , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyang","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhang","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"Zibo Key Laboratory of Synthetic Biology and New Pharmaceutical Applications , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuetong","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"Zibo Key Laboratory of Synthetic Biology and New Pharmaceutical Applications , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiutao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology , 255000 Zibo ,","place":["China"]},{"name":"Zibo Key Laboratory of Synthetic Biology and New Pharmaceutical Applications , 255000 Zibo ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"2026102205284511700_bib1","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3390\/ijms19010048","article-title":"Carbon catabolite repression in filamentous fungi","volume":"19","author":"Adnan","year":"2017","journal-title":"Int J Mol Sci"},{"key":"2026102205284511700_bib2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2014\/317092","article-title":"Characterization of \u03b2-Glucosidase produced by Aspergillus niger under solid-state fermentation and partially purified using MANAE-Agarose","volume":"2014","author":"Baraldo\u00a0Junior","year":"2014","journal-title":"Biotechnol Res Int"},{"key":"2026102205284511700_bib3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-94-007-6724-9_70-1","article-title":"Strategies for overcoming the inhibition of cellulose hydrolysis","volume-title":"Handbook of Biorefinery Research and Technology","author":"Chauhan","year":"2024"},{"key":"2026102205284511700_bib4","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1016\/s0958-1669(03)00099-5","article-title":"Directed evolution of industrial enzymes: an update","volume":"14","author":"Cherry","year":"2003","journal-title":"Curr Opin Biotechnol"},{"key":"2026102205284511700_bib5","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1002\/fsn3.852","article-title":"Optimization of cellulase production under solid-state fermentation by a new mutant strain of Trichoderma reesei","volume":"7","author":"Darabzadeh","year":"2018","journal-title":"Food Sci Nutr"},{"key":"2026102205284511700_bib6","doi-asserted-by":"publisher","first-page":"4959","DOI":"10.1007\/s00253-016-7342-x","article-title":"Comparison of catalytic properties of multiple \u03b2-glucosidases of Trichoderma reesei","volume":"100","author":"Guo","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"2026102205284511700_bib7","doi-asserted-by":"publisher","first-page":"e62631","DOI":"10.1371\/journal.pone.0062631","article-title":"Systems analysis of lactose metabolism in Trichoderma reesei identifies a lactose permease that is essential for cellulase induction","volume":"8","author":"Ivanova","year":"2013","journal-title":"PLoS One"},{"key":"2026102205284511700_bib8","doi-asserted-by":"publisher","first-page":"1284658","DOI":"10.3389\/fpls.2023.1284658","article-title":"Editorial: understanding plant cell wall recalcitrance for efficient lignocellulose processing","volume":"14","author":"Klose","year":"2023","journal-title":"Front Plant Sci"},{"key":"2026102205284511700_bib9","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1134\/s0006297909050137","article-title":"Isolation and properties of fungal beta-glucosidases","volume":"74","author":"Korotkova","year":"2009","journal-title":"Biochemistry"},{"key":"2026102205284511700_bib10","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-016-0550-3","article-title":"a \u03b2-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production","volume":"15","author":"Li","year":"2016","journal-title":"Microb Cell Fact"},{"key":"2026102205284511700_bib11","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1186\/s40643-021-00411-4","article-title":"Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and \u03b2-disaccharide","volume":"8","author":"Li","year":"2021","journal-title":"Bioresour Bioprocess"},{"key":"2026102205284511700_bib12","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.jcis.2024.11.188","article-title":"Unveiling the mechanisms of mixed surfactant synergy in passivating lignin-cellulase interactions during lignocellulosic saccharification","volume":"681","author":"Liu","year":"2025","journal-title":"J Colloid Interface Sci"},{"key":"2026102205284511700_bib13","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2023.129974","article-title":"Improving enzymatic efficiency of \u03b2-glucosidases in cellulase system by altering its binding behavior to the insoluble substrate during bioconversion of lignocellulose","volume":"391","author":"Lu","year":"2024","journal-title":"Bioresour Technol"},{"key":"2026102205284511700_bib14","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.biochi.2024.05.009","article-title":"Microbial \u03b2-glucosidases: recent advances and applications","volume":"225","author":"Magwaza","year":"2024","journal-title":"Biochimie"},{"key":"2026102205284511700_bib15","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.molcatb.2015.08.014","article-title":"Enzymatic preparation of oligosaccharides by transglycosylation: a comparative study of glucosidases","volume":"122","author":"Mangas-S\u00e1nchez","year":"2015","journal-title":"J Mol Catal B: Enzym"},{"key":"2026102205284511700_bib16","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.3390\/ijms26083731","article-title":"Optimization and bioreactor scale-up of cellulase production in Trichoderma sp. KMF006 for higher yield and performance","volume":"26","author":"Myeong","year":"2025","journal-title":"Int J Mol Sci"},{"key":"2026102205284511700_bib17","doi-asserted-by":"publisher","first-page":"20","DOI":"10.2174\/1875398100902010020","article-title":"Discaccharide synthesis by enzymatic condensation of glucose: glycoside linkage patterns for different fungal species","volume":"2","author":"Semenova","year":"2009","journal-title":"Open Glycoscie"},{"key":"2026102205284511700_bib18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-016-0467-x","article-title":"Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts","volume":"15","author":"Signori","year":"2016","journal-title":"Microb Cell Fact"},{"key":"2026102205284511700_bib19","doi-asserted-by":"publisher","DOI":"10.1016\/j.rser.2021.111258","article-title":"Lignocellulosic biorefineries: the current state of challenges and strategies for efficient commercialization","volume":"148","author":"Usmani","year":"2021","journal-title":"Renew Sustain Energy Rev"},{"key":"2026102205284511700_bib20","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1038\/s41586-020-1937-1","article-title":"Synthesis of rare sugar isomers through site-selective epimerization","volume":"578","author":"Wang","year":"2020","journal-title":"Nature"},{"key":"2026102205284511700_bib21","doi-asserted-by":"publisher","first-page":"131402","DOI":"10.1016\/j.biortech.2024.131402","article-title":"Efficient production of sophorose from glucose and its potentially industrial application in cellulase production","volume":"412","author":"Yang","year":"2024","journal-title":"Bioresour Technol"},{"key":"2026102205284511700_bib22","doi-asserted-by":"publisher","first-page":"S93","DOI":"10.1016\/j.sjbs.2015.06.018","article-title":"Measurement of filter paper activities of cellulase with microplate-based assay","volume":"23","author":"Yu","year":"2016","journal-title":"Saudi J Biol Sci"},{"key":"2026102205284511700_bib23","doi-asserted-by":"publisher","first-page":"17392","DOI":"10.1039\/d2ra01192a","article-title":"Induction of cellulase production in Trichoderma reesei by a glucose\u2013sophorose mixture as an inducer prepared using stevioside","volume":"12","author":"Zhang","year":"2022","journal-title":"RSC Adv"},{"key":"2026102205284511700_bib24","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1360\/02yc0163","article-title":"Mechanism of cellobiose inhibition in cellulose hydrolysis by cellobiohydrolase","volume":"47","author":"ZHAO","year":"2004","journal-title":"Sci China Ser C"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovag088\/71947222\/ovag088.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/10\/ovag088\/71947222\/ovag088.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/10\/ovag088\/71947222\/ovag088.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:28:52Z","timestamp":1792661332000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovag088\/8916484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovag088","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,10]]},"published":{"date-parts":[[2026,10]]},"article-number":"ovag088"}}