{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:08:45Z","timestamp":1753877325961,"version":"3.41.2"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pharyngeal pouches, which are endodermal outpockets that segment the pharyngeal arches, play a crucial role in the development of craniofacial skeletons in vertebrate embryos. Our previous study successfully identified pharyngeal pouch progenitors (PPPs) in zebrafish embryos and emphasized the significance of BMP2b signaling in their specification. However, the specific mechanism by which these progenitors originate from endodermal cells remains largely unknown. Here we found that the pharmacological activation of Wnt signaling pathway disrupts the emergence of PPPs and subsequently hinders the formation of pharyngeal pouches. Moreover, we have identified the expression of tmem88a and tmem88b (collectively known as tmem88a\/b) in PPPs during the early-somite stages. Furthermore, the deficiency of tmem88a\/b leads to an excessive accumulation of \u03b2-catenin in both the cytoplasm and nucleus of endodermal cells that are intended to differentiate into PPPs. Importantly, suppressing the hyperactivation of Wnt\/\u03b2-catenin signaling through pharmacological treatment, the defects in PPP specification in tmem88a\/b\u2212\/\u2212 mutants are successfully rescued. In summary, our findings establish a clear connection between the specification of PPPs and the regulation of Wnt signaling mediated by Tmem88. These results underscore the pivotal role of Tmem88 in the development of pharyngeal pouches.<\/jats:p>","DOI":"10.1093\/lifemedi\/lnad044","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T18:31:00Z","timestamp":1700245860000},"source":"Crossref","is-referenced-by-count":2,"title":["Tmem88 plays an essential role in pharyngeal pouch progenitor specification by inhibiting Wnt\/\u03b2-catenin signaling"],"prefix":"10.1093","volume":"2","author":[{"given":"Jingwen","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences , Beijing 100101 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Yang","sequence":"additional","affiliation":[{"name":"Innovation Centre of Ministry of Education for Development and Diseases, The Sixth Affiliated Hospital, School of Medicine, South China University of Technology , Guangzhou 510006 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zidong","family":"Lu","sequence":"additional","affiliation":[{"name":"Innovation Centre of Ministry of Education for Development and Diseases, The Sixth Affiliated Hospital, School of Medicine, South China University of Technology , Guangzhou 510006 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Innovation Centre of Ministry of Education for Development and Diseases, The Sixth Affiliated Hospital, School of Medicine, South China University of Technology , Guangzhou 510006 , 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