{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:09:57Z","timestamp":1784131797782,"version":"3.55.0"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study investigated the cardiac development of the Amazonian turtle Podocnemis unifilis during its embryonic phase, from the 5th to the 59th day of incubation, with emphasis on the volumetric dynamics estimated by stereology. Development was organized into six morphological stages (E1\u2013E6), from the folding of the heart tube to the formation of the prehatching heart. In the early stages, the heart showed a predominance of the myocardium (44%), mesenchyme (27%), and cardiac jelly (27%), which are components that undergo progressive regression during development. In contrast, in the advanced stages, expansion of the myocardium (up to 70%) and of the lumen (up to 27%) was observed, in addition to the late appearance of the atrioventricular valves (3%) and cartilage (1%). Analysis of the absolute volumes corroborated this finding, with an increase in the total heart volume from 0.015\u2005mm3 (E1) to 1.33\u2005mm3 (E6). Multivariate analysis (PCA) (i) clearly distinguished the heart components of the early stages from those close to hatching, (ii) statistically validated that the developmental stages represent distinct phases, and (iii) showed that the main change between them is the transition from a heart rich in jelly to a heart rich in myocardium and lumen. Together, the integration of embryological and stereological data provides a detailed quantitative reference of cardiogenesis in P. unifilis.<\/jats:p>","DOI":"10.1093\/mam\/ozag069","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:44:17Z","timestamp":1784130257000},"source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Analysis of the Heart Development of\n                    <i>Podocnemis unifilis<\/i>\n                    (Podocnemididae, Testudines): A Stereology Study"],"prefix":"10.1093","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7941-5166","authenticated-orcid":false,"given":"Layla Ianca Queiroz","family":"Rocha","sequence":"first","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology, Department of Animal Sciences, Federal Rural University of the Semi-Arid , Av. Francisco Mota, 572, Costae Silva, Mossor\u00f3, RN 59.625-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Fabiele Silva","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal and Comparative Anatomy, Department of Morphology, Federal University of Amazonas , Av. General Rodrigo Octavio Jord\u00e3o Ramos, 1200, Coroado I, Manaus, AM 69067-005 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9919-4831","authenticated-orcid":false,"given":"M\u00e1rcio Santos de Oliveira","family":"Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology, Department of Animal Sciences, Federal Rural University of the Semi-Arid , Av. Francisco Mota, 572, Costae Silva, Mossor\u00f3, RN 59.625-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Castanhola","family":"Dias","sequence":"additional","affiliation":[{"name":"Thematic Laboratory of Microscopy and Nanotechnology\/LTMN, National Institute of Amazonian Research , Av. Andr\u00e9 Ara\u00fajo, 2936, Petr\u00f3polis, Manaus, AM 69067-375 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1550-8969","authenticated-orcid":false,"given":"Oscar Tadeu Ferreira","family":"da Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Quantitative Morphology, Department of Morphology, Institute of Biological Sciences, Federal University of Amazonas , Av. General Rodrigo Octavio Jord\u00e3o Ramos, 1200, Coroado I, Manaus, AM 69067-005 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology, Department of Animal Sciences, Federal Rural University of the Semi-Arid , Av. Francisco Mota, 572, Costae Silva, Mossor\u00f3, RN 59.625-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6295-7436","authenticated-orcid":false,"given":"Marcela dos Santos","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Animal and Comparative Anatomy, Department of Morphology, Federal University of Amazonas , Av. General Rodrigo Octavio Jord\u00e3o Ramos, 1200, Coroado I, Manaus, AM 69067-005 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7960-5373","authenticated-orcid":false,"given":"Carlos Eduardo Bezerra","family":"de Moura","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology, Department of Animal Sciences, Federal Rural University of the Semi-Arid , Av. 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