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For immunohistochemistry, myelin-associated glycoprotein (MAG), neurofilament 200 (NF200), calbindin D-28K, and glial fibrillary-associated protein (GFAP) were investigated. The cerebellar cortex in both species was composed of three cellular layers: molecular, granular, and Purkinje cell (PC) layers. Purkinje cells in the porcupine showed a purple-colored and dark blue-colored cytoplasm in reaction to the crystal violet and Luxol fast blue staining, respectively. In the guinea pig, PC has a uniform reaction to the Luxol fast blue with dark-blue\u2013colored cytoplasm. However, in response to the crystal violet, some PC with dark-purple cytoplasm showed stronger reaction than other PC which showed light-purple cytoplasm. The PC layer in some folia of the porcupine cerebellum was composed of 2\u20133 layers. The expression rates of calbindin D-28K, MAG, GFAP, and NF200 in the porcupine cerebellum were determined to be 19%, 42.5%, 62%, and 30%, respectively. These values were determined to be 27%, 34%, 43.5%, and 31.5%, respectively, in the guinea pig cerebellum.<\/jats:p>","DOI":"10.1093\/micmic\/ozad043","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T10:01:10Z","timestamp":1680256870000},"page":"1289-1297","source":"Crossref","is-referenced-by-count":0,"title":["Histological and Immunohistochemical Investigation of the Cerebellum in Porcupine and Guinea Pig"],"prefix":"10.1093","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4704-6076","authenticated-orcid":false,"given":"Nader","family":"Goodarzi","sequence":"first","affiliation":[{"name":"Department of Basic Sciences and Pathobiology, Faculty of Veterinary Medicine, Razi University , Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoumeh","family":"Nowrozi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Razi University , Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Baharan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Razi University , Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"2023060912172758000_ozad043-B1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0304-3940(92)90917-V","article-title":"Localization of gene expression of calbindin in the brain of adult rats","volume":"138","author":"Abe","year":"1992","journal-title":"Neurosci Lett"},{"issue":"3","key":"2023060912172758000_ozad043-B2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF00830199","article-title":"Dark and clear Purkinje's cells in the cerebellum during the postresuscitation period","volume":"95","author":"Avrushchenko","year":"1983","journal-title":"Bull Exp Biol Med"},{"key":"2023060912172758000_ozad043-B3","doi-asserted-by":"crossref","first-page":"131","DOI":"10.17221\/5605-VETMED","article-title":"The morphology of circulus arteriosus cerebri in the porcupine (Hystrix cristata)","volume":"50","author":"Aydin","year":"2005","journal-title":"Vet Med"},{"issue":"2","key":"2023060912172758000_ozad043-B4","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1002\/glia.22431","article-title":"Differential glial activation during the degeneration of Purkinje cells and mitral cells in the PCD mutant mice","volume":"61","author":"Baltanas","year":"2013","journal-title":"Glia"},{"key":"2023060912172758000_ozad043-B5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0006-8993(97)00484-8","article-title":"Increased number of unmyelinated axons in optic nerves of adult mice deficient in the myelin-associated glycoprotein (MAG)","volume":"762","author":"Bartsch","year":"1997","journal-title":"Brain Res"},{"key":"2023060912172758000_ozad043-B6","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1080\/10253890.2017.1378637","article-title":"Maternal stress in pregnancy affects myelination and neurosteroid regulatory pathways in the guinea pig cerebellum","volume":"20","author":"Bennett","year":"2017","journal-title":"Stress"},{"key":"2023060912172758000_ozad043-B7","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.neuron.2013.10.044","article-title":"The cerebellum and cognitive function: 25 years of insight from anatomy and neuroimaging","volume":"80","author":"Buckner","year":"2013","journal-title":"Neuron"},{"key":"2023060912172758000_ozad043-B8","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1177\/0300985814561270","article-title":"Vitamin C depletion in prenatal guinea pigs as a model of lissencephaly type II","volume":"52","author":"\u010capo","year":"2015","journal-title":"Vet Pathol"},{"year":"2010","author":"Castej\u00f3n","key":"2023060912172758000_ozad043-B9"},{"key":"2023060912172758000_ozad043-B10","first-page":"29","article-title":"Correlative microscopy of Purkinje dendritic spines. 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