{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T19:22:38Z","timestamp":1785093758562,"version":"3.55.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The leaves of Monteverdia ilicifolia (syn. Maytenus ilicifolia), commonly called espinheira-santa, are widely used in South American traditional medicines to treat gastritis and ulcers. Several products labeled as espinheira-santa are sold as dietary supplements in retail stores and via e-commerce. Many different species with similar leaf morphology are often mistaken for Monteverdia ilicifolia and used as espinheira-santa, including Monteverdia aquifolia (Celastraceae), Citronella gongonha (Cardiopteridaceae), Jodina rhombifolia (Santalaceae), Sorocea bonplandii (Moraceae), and Zollernia ilicifolia (Fabaceae). This study aimed to characterize M. ilicifolia and distinguish it from adulterants using morphological and microscopic techniques. In addition, foreign matter and powder characteristics of botanical materials sold as \u201cespinheira-santa\u201d were analyzed. The morphoanatomical studies of the leaves and stems of M. ilicifolia and its five adulterant species have revealed noteworthy features that can help species identification and quality control of commercial espinheira-santa. This study showed that many commercial espinheira-santa materials were adulterated and of inferior quality.<\/jats:p>","DOI":"10.1093\/micmic\/ozad098","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T20:37:32Z","timestamp":1693255052000},"page":"1809-1821","source":"Crossref","is-referenced-by-count":11,"title":["Authentication and Quality Control of the Brazilian Traditional Herb \u201cEspinheira-Santa\u201d (<i>Monteverdia ilicifolia<\/i>) by Morpho-Anatomy and Microscopy"],"prefix":"10.1093","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3209-1125","authenticated-orcid":false,"given":"Kevin A","family":"Antunes","sequence":"first","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane M","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-8184","authenticated-orcid":false,"given":"Valter P","family":"Almeida","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irailson T","family":"Monchak","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilmer H","family":"Perera","sequence":"additional","affiliation":[{"name":"CAMAG Scientific, Inc., 515 Cornelius Harnett Dr, Wilmington, NC 28401, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Heiden","sequence":"additional","affiliation":[{"name":"Embrapa Clima Temperado, Pelotas, BR-392, km 78 Monte Bonito, RS 96010-971, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8286-4889","authenticated-orcid":false,"given":"Ernestino S G","family":"Guarino","sequence":"additional","affiliation":[{"name":"Embrapa Clima Temperado, Pelotas, BR-392, km 78 Monte Bonito, RS 96010-971, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera L P","family":"Santos","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Bioci\u00eancias, Meio Ambiente e Humanidades, Centro Universit\u00e1rio Internacional Uninter , Curitiba, PR 80410-150 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo V","family":"Farago","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"},{"name":"National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, P.O. Box 1848, MS 38677 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7368-9644","authenticated-orcid":false,"given":"Vijayasankar","family":"Raman","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, P.O. Box 1848, MS 38677 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikhlas A","family":"Khan","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, P.O. Box 1848, MS 38677 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Manfron","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa , 4748 Carlos Cavalcanti Avenue, Ponta Grossa, PR 84030-900 , Brazil"},{"name":"National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, P.O. Box 1848, MS 38677 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"key":"2023100116022716500_ozad098-B1","volume-title":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria","author":"ANVISA","year":"2019","edition":"6 ed"},{"key":"2023100116022716500_ozad098-B2","doi-asserted-by":"crossref","DOI":"10.2307\/2418781","volume-title":"Botanical Microtechnique and Cytochemistry","author":"Berlyn","year":"1976"},{"issue":"8","key":"2023100116022716500_ozad098-B3","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1002\/jemt.22870","article-title":"Microscopic diagnosis of the leaf and stem of Piper solmsianum C.DC","volume":"80","author":"Bertocco","year":"2017","journal-title":"Microsc Res Tech"},{"issue":"4","key":"2023100116022716500_ozad098-B4","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1600\/036364417X696456","article-title":"Systematics of new world Maytenus (Celastraceae) and a new delimitation of the genus","volume":"42","author":"Biral","year":"2017","journal-title":"Syst Bot"},{"issue":"8","key":"2023100116022716500_ozad098-B5","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1002\/jemt.23045","article-title":"Foliar anatomy and microscopy of six Brazilian species of Baccharis (Asteraceae)","volume":"81","author":"Budel","year":"2018","journal-title":"Microsc Res Tech"},{"issue":"2","key":"2023100116022716500_ozad098-B6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s43450-020-00123-4","article-title":"Microscopy of Eugenia involucrata, chemical composition and biological activities of the volatile oil","volume":"31","author":"D\u2019Almeida","year":"2021","journal-title":"Rev Bras Farmacogn"},{"issue":"5","key":"2023100116022716500_ozad098-B7","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1017\/S1431927621012447","article-title":"Microscopy and histochemistry of leaves and stems of Baccharis subgenus Coridifoliae (Asteraceae) through LM and SEM-EDS","volume":"27","author":"de Almeida","year":"2021","journal-title":"Microsc Microanal"},{"issue":"5","key":"2023100116022716500_ozad098-B8","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1017\/S1431927621012514","article-title":"Light and scanning electron microscopy, energy dispersive X-ray spectroscopy, and histochemistry of Eucalyptus tereticornis","volume":"27","author":"de Brito","year":"2021","journal-title":"Microsc Microanal"},{"issue":"5","key":"2023100116022716500_ozad098-B9","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1007\/s43450-021-00214-w","article-title":"Anti-adhesive activity of Maytenus ilicifolia against Helicobacter pylori","volume":"31","author":"de Paula","year":"2021","journal-title":"Rev Bras Farmacogn"},{"issue":"1","key":"2023100116022716500_ozad098-B10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.fitote.2004.10.003","article-title":"Stem and leaf morphoanatomy of Maytenus ilicifolia","volume":"76","author":"Duarte","year":"2005","journal-title":"Fitoterapia"},{"issue":"2","key":"2023100116022716500_ozad098-B11","first-page":"268","article-title":"Alca\u00e7uz e espinheira-santa no tratamento de gastrite","volume":"2","author":"Filho","year":"2019","journal-title":"Revista de Inicia\u00e7\u00e3o Cient\u00edfica e Extens\u00e3o"},{"key":"2023100116022716500_ozad098-B12","volume-title":"Practical Plant Anatomy","author":"Foster","year":"1949"},{"issue":"3","key":"2023100116022716500_ozad098-B13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3109\/10520296309067156","article-title":"Fuchsin staining with NaOH clearing for lignified elements of whole plants or plant organs","volume":"38","author":"Fuchs","year":"1963","journal-title":"Stain Technol"},{"key":"2023100116022716500_ozad098-B14","volume-title":"Techniques Histologiques","author":"Gabe","year":"1968"},{"key":"2023100116022716500_ozad098-B15","author":"Jacomassi","year":"2003"},{"key":"2023100116022716500_ozad098-B16","volume-title":"Plant Microtechnique","author":"Johansen","year":"1940"},{"issue":"5","key":"2023100116022716500_ozad098-B17","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3109\/10520299809141117","article-title":"Astra blue and basic fuchsin double staining of plant materials","volume":"73","author":"Kraus","year":"1998","journal-title":"Biotech Histochem"},{"key":"2023100116022716500_ozad098-B18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2022.115255","article-title":"Small conductance calcium-activated potassium channels and nitric oxide\/cGMP pathway mediate the cardioprotective effects of Croton urucurana Baill. in hypertensive rats","volume":"293","author":"Lopes","year":"2022","journal-title":"J Ethnopharmacol"},{"issue":"3","key":"2023100116022716500_ozad098-B19","doi-asserted-by":"crossref","first-page":"212","DOI":"10.3390\/life11030212","article-title":"Atheroprotective properties of Costus spicatus (Jacq.) 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