{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:48:06Z","timestamp":1740170886058,"version":"3.37.3"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3-4","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019MH115"],"award-info":[{"award-number":["ZR2019MH115"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Introduction<\/jats:title>\n                  <jats:p>This was an in vivo animal study designed to investigate the interaction between dexamethasone (Dex) and microRNA-204 (miR-204) in a mouse alkali burn-induced corneal neovascularization (CNV) model. The function of miR-204 was then investigated in human mammary epithelial cells (HMECs) in vitro.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and Methods<\/jats:title>\n                  <jats:p>The CNV model was induced by corneal alkali burn in BLAB\/c mice. The mice were randomly divided into five groups: normal control (Ctrl), alkali burn-induced corneal injury (Alkali), alkali burn\u2009+\u2009Dex (Dex), alkali burn\u2009+\u2009negative control (NTC), and alkali burn\u2009+\u2009miR-204 agomir (miR-204). Subconjunctival injection of NTC, Dex, or miR-204 agomir was conducted at 0, 3, and 6\u2009days, respectively, after alkali burn. The corneas were collected at day 7 after injury, and the CNV area was observed using immunofluorescence staining. The expression of miR-204 was analyzed with quantitative real time (qRT)-PCR. In HMECs, exogenous miR-204 agomir or antagomir was used to strengthen or inhibit the expression of miR-204. Migration assays and tube formation studies were conducted to evaluate the function of miR-204 on HMECs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>At 7\u2009days post-alkali burn, CNV grew aggressively into the cornea. MicroRNA-204 expression was reduced in the Alkali group in contrast with the Ctrl group (P\u2009=\u2009.003). However, miR-204 was upregulated in the Dex group (vs. alkali group, P\u2009=\u2009.008). The CNV areas in the NTC and miR-204 groups were 59.30\u2009\u00b1\u20098.32% and 25.60\u2009\u00b1\u20092.30%, respectively (P\u2009=\u2009.002). In vitro, miR-204 agomir showed obvious inhibition on HMEC migration in contrast with NTC (P\u2009=\u2009.033) and miR-204 antagomir (P\u2009=\u2009.017). Compared with NTC, miR-204 agomir attenuated tube formation, while miR-204 antagomir accelerated HMEC tube formation (P\u2009&amp;lt;\u2009.05).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The role of Dex in attenuating CNV may be partly attributed to miR-204. MiR-204 may be a potential therapeutic target in alkali burn-induced CNV.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/milmed\/usac260","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T06:48:23Z","timestamp":1661928503000},"page":"627-631","source":"Crossref","is-referenced-by-count":0,"title":["Dexamethasone and MicroRNA-204 Inhibit Corneal Neovascularization"],"prefix":"10.1093","volume":"189","author":[{"given":"Xiaoping","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong 266002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong 266002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong 266002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"BS","given":"Rui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong 266002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"issue":"3","key":"2024022721585006000_R1","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.jtos.2019.04.002","article-title":"MicroRNAs in the cornea: role and implications for treatment of corneal neovascularization","volume":"17","author":"Mukwaya","year":"2019","journal-title":"Ocul Surf"},{"issue":"7114","key":"2024022721585006000_R2","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1038\/nature05249","article-title":"Corneal avascularity is due to soluble VEGF receptor-1","volume":"443","author":"Ambati","year":"2006","journal-title":"Nature"},{"key":"2024022721585006000_R3","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.exer.2017.12.001","article-title":"Epithelium-derived miR-204 inhibits corneal neovascularization","volume":"167","author":"Zhang","year":"2018","journal-title":"Exp Eye Res"},{"issue":"4","key":"2024022721585006000_R4","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.jtos.2018.06.004","article-title":"Current and emerging therapies for corneal neovascularization","volume":"16","author":"Roshandel","year":"2018","journal-title":"Ocul Surf"},{"issue":"4","key":"2024022721585006000_R5","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/j.survophthal.2017.12.008","article-title":"Angiogenesis and lymphangiogenesis in corneal transplantation-a review","volume":"63","author":"Zhong","year":"2018","journal-title":"Surv Ophthalmol"},{"key":"2024022721585006000_R6","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2017.111","article-title":"Genome-wide expression datasets of anti-VEGF and dexamethasone treatment of angiogenesis in the rat cornea","volume":"4","author":"Mukwaya","year":"2017","journal-title":"Sci Data"},{"key":"2024022721585006000_R7","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.biomaterials.2017.02.016","article-title":"Subconjunctival injectable dendrimer-dexamethasone gel for the treatment of corneal inflammation","volume":"125","author":"Soiberman","year":"2017","journal-title":"Biomaterials"},{"issue":"4","key":"2024022721585006000_R8","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1038\/sj.eye.6701895","article-title":"Corticosteroid-induced glaucoma: a review of the literature","volume":"20","author":"Kersey","year":"2006","journal-title":"Eye (Lond)"},{"issue":"2","key":"2024022721585006000_R9","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1111\/j.1444-0938.2002.tb03011.x","article-title":"What causes steroid cataracts? 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