{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:40:50Z","timestamp":1786120850525,"version":"build-2736575974"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"Supplement_1","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100014055","name":"United States Army Medical Research Acquisition Activity","doi-asserted-by":"publisher","award":["HT9425-24-1-0600"],"award-info":[{"award-number":["HT9425-24-1-0600"]}],"id":[{"id":"10.13039\/100014055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Current storage methods for red blood cell (RBC) products require refrigeration or freezing, but this can pose significant issues in resource-constrained environments or during national emergencies where there is no access to cold storage equipment. To address this challenge, we are developing a new freeze-dried RBC product that can be stored in a dried state at ambient temperatures for long periods of time. A recently developed freeze-dried RBC product was shown to bind oxygen effectively, but increased hemolysis can occur during the freeze-drying process, which can cause toxicity during transfusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>To mitigate the risk of adverse effects associated with free hemoglobin in transfusions of freeze-dried blood products, this study explores in-line post-processing methods that utilize chelating resin beads to effectively adsorb cell-free hemoglobin. This approach could avoid the limitations of traditional centrifugation-based free hemoglobin removal procedures, which may not be feasible in some settings where freeze-dried RBC products could be utilized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Non-fluidic and fluidic filtration of RBCs suspended in hemoglobin solutions decreased the amount of cell-free hemoglobin by approximately 80%-90% with no significant decreases in RBC counts. Filtration of freeze-dried and rehydrated RBCs using chelating resin beads as an adsorption matrix reduced free hemoglobin levels by more than 60% compared to freeze-dried and rehydrated RBC solutions without filtration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results demonstrate the efficacy of using chelating resin beads as a post-processing technique to reduce the risk of hemolysis-mediated injury from freeze-dried RBC products. These findings are a key step toward developing effective methods to produce freeze-dried red blood cell products that are safe for transfusion in austere, resource-limited environments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/milmed\/usag271","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:36:58Z","timestamp":1780400218000},"page":"723-730","source":"Crossref","is-referenced-by-count":0,"title":["Reducing Cell-Free Hemoglobin Levels in Freeze-dried Red Blood Cells for Transfusions in Austere Environments"],"prefix":"10.1093","volume":"191","author":[{"given":"John T","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Bioengineering, University of Louisville , Louisville, KY 40292,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor S","family":"Centner","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Louisville , 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