{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:27:03Z","timestamp":1787009223015,"version":"build-2736575974"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25461516"],"award-info":[{"award-number":["25461516"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K09939"],"award-info":[{"award-number":["16K09939"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108094"],"award-info":[{"award-number":["JP19fk0108094"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108052"],"award-info":[{"award-number":["JP19fk0108052"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mucormycosis is a deep-seated fungal infection that mainly develops in patients with severe immunodeficiencies such as those with malignant hematological diseases. Despite poor prognosis, there is no reliable and minimally invasive diagnostic method\u2014such as serodiagnosis\u2014for making a clinical decision regarding the condition. As early diagnosis and early treatment improve the prognosis of mucormycosis, the development of a sensitive early diagnostic method is important. We had previously identified a Rhizopus-specific antigen (RSA) by signal sequence trapping and retrovirus-mediated expression (SST-REX), and evaluated its utility as a diagnostic antigen by constructing a sandwich enzyme-linked immunosorbent assay (ELISA) system to detect serum RSA levels in inoculated mice. In this study, we used the RSA-specific rabbit monoclonal antibodies generated by novel hybridoma technology to improve the sensitivity of the ELISA system. We observed an increase in serum and bronchoalveolar lavage fluid (BALF) levels of RSA in mouse model 1 day after inoculation, suggesting that this newly developed monoclonal antibody-based ELISA system may be useful for the diagnosis of mucormycosis in the early stages of infection. In addition, we measured RSA levels in human serum and BALF, and found that serum RSA level was higher in mucormycosis patients (15.1\u00a0ng\/ml) than that in invasive pulmonary aspergillosis patients (0.53\u00a0ng\/ml) and the negative control (0.49\u00a0ng\/ml). Our results suggest that RSA may be a powerful tool for the diagnosis of pulmonary mucormycosis, and its differentiation from other deep-seated mycoses such as aspergillosis.<\/jats:p>","DOI":"10.1093\/mmy\/myaa001","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T07:07:39Z","timestamp":1578208059000},"page":"958-964","source":"Crossref","is-referenced-by-count":10,"title":["Detection of Rhizopus-specific antigen in human and murine serum and bronchoalveolar lavage"],"prefix":"10.1093","volume":"58","author":[{"given":"Wataru","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Infection Control Science, Osaka City University Graduate School of Medicine, Osaka, Japan"},{"name":"Department of Respiratory Medicine, Osaka City University Graduate School of Medicine, Osaka, Japan"},{"name":"Research Center for Infectious Disease Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamiko","family":"Niki","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Osaka City University Graduate School of Medicine, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Osaka City University Graduate School of Medicine, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Infection Control Science, Osaka City University Graduate School of Medicine, Osaka, Japan"},{"name":"Research Center for Infectious Disease Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Infection Control Science, Osaka City University Graduate School of 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