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The N-terminal sequence obtained from the purified protein was used to isolate a clone containing the full-length coding sequence from a C. neoformans var. neoformans (strain B-3501A) cDNA library. Bioinformatics analysis indicated that this gene is present, with variable homology, in all sequenced genomes of the C. neoformans\/C. gattii species complex. The cDNA clone was used to produce a recombinant 45\u00a0kDa protein in E. coli that displayed the ability to convert carboxymethyl cellulose and was therefore designated as NG-Case (standing for Neoformans Gattii Cellulase). To explore its potential use as a vaccine candidate, the recombinant protein was used to immunize mice and was found capable of inducing T helper type 1 responses and delayed-type hypersensitivity reactions, but not immune protection against a highly virulent C. neoformans var grubii strain. These data may be useful to better understand the mechanisms underlying the ability C. neoformans\/C. gattii to colonize plant habitats and to interact with the human host during infection.<\/jats:p>","DOI":"10.1093\/mmy\/myaa012","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T20:14:41Z","timestamp":1582661681000},"page":"1138-1148","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of an immunogenic cellulase secreted by Cryptococcus pathogens"],"prefix":"10.1093","volume":"58","author":[{"given":"Angelina","family":"Midiri","sequence":"first","affiliation":[{"name":"Department of Human Pathology, University of Messina, Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Mancuso","sequence":"additional","affiliation":[{"name":"Department of Human Pathology, University of Messina, Messina, 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