{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:47:12Z","timestamp":1789577232265,"version":"build-2803163510"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,4,11]],"date-time":"2020-04-11T00:00:00Z","timestamp":1586563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to evaluate a colorimetric method, MIRONAUT-AM, for determining susceptibility testing of anidulafungin, amphotericin, voriconazole, and itraconazole by comparing the minimum inhibitory (effective) concentrations (MICs\/MECs) obtained by this method to those generated by the reference Clinical Laboratory Standard Institute (CLSI) broth microdilution method. In sum, 78 clinical isolates of Aspergillus species, nine of them non-wild type (non-WT) with itraconazole MIC ranging from 2\u00a0mg\/l to &amp;gt;16\u00a0mg\/l, were tested against above antifungals. A. fumigatus ATCC 204305 was used as a reference strain, and test was performed in accordance with slightly modified yeast susceptibility testing instruction of the manufacture; conidia suspension inoculum and alamarBlue concentration were optimized. These same isolates were referred to Bristol Mycology reference laboratory and tested by CLSI method. The MICs and MECs generated by the two methods were compared using concordance analysis. MIRONAUT-AM showed significant concordance (P\u00a0&amp;lt;\u00a0.0001) with CLSI method, and overall agreement was high (\u226590%). In addition, MIRONAUT-AM produced echinocandin MECs results within 18\u201324\u00a0hours incubation time and correctly detected all non-WT isolates except one isolate. This colorimetric method is very promising and appears to be a suitable alternative susceptibility testing method to labor intensive broth microdilution reference method for Aspergillus species.<\/jats:p>","DOI":"10.1093\/mmy\/myaa020","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T00:05:12Z","timestamp":1585872312000},"page":"1085-1090","source":"Crossref","is-referenced-by-count":9,"title":["Comparative Evaluation of MIRONAUT-AM and CLSI broth microdilution method for antifungal susceptibility testing of Aspergillus species against four commonly used antifungals"],"prefix":"10.1093","volume":"58","author":[{"given":"Ali","family":"Nuh","sequence":"first","affiliation":[{"name":"Department of Microbiology, Laboratory Medicine, Royal Brompton and Harefield NHS foundation trust, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Newara","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Laboratory Medicine, Royal Brompton and Harefield NHS foundation trust, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Schelenz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Kings College Hospital, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darius","family":"Armstrong-James","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Laboratory Medicine, Royal Brompton and Harefield NHS foundation trust, London"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,4,11]]},"reference":[{"key":"2020111110125173000_bib1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1136\/thoraxjnl-2014-206291","article-title":"The clinical spectrum of pulmonary aspergillosis","volume":"70","author":"Kosmidis","year":"2015","journal-title":"Thorax"},{"key":"2020111110125173000_bib2","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1055\/s-0035-1562893","article-title":"Invasive aspergillosis: epidemiology, clinical aspects, and treatment","volume":"36","author":"Gregg","year":"2015","journal-title":"Semin Respir Crit Care Med"},{"key":"2020111110125173000_bib3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s13054-014-0722-7","article-title":"Epidemiology of invasive aspergillosis in critically ill patients: clinical presentation, underlying conditions, and outcomes","volume":"19","author":"Taccone","year":"2015","journal-title":"Crit Care"},{"key":"2020111110125173000_bib4","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S1473-3099(15)70006-X","article-title":"British Society for Medical Mycology best practice recommendations for the diagnosis of serious fungal diseases","volume":"15","author":"Schelenz","year":"2015","journal-title":"Lancet Infect Dis"},{"key":"2020111110125173000_bib5","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.cmi.2018.11.027","article-title":"Triazole resistance in Aspergillus fumigatus: recent insights and challenges for patient management","volume":"25","author":"Lestrade","year":"2019","journal-title":"Clin. 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