{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:47:10Z","timestamp":1783108030387,"version":"3.54.6"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T00:00:00Z","timestamp":1614124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["#2019\/10261-2"],"award-info":[{"award-number":["#2019\/10261-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["16\/11198-4"],"award-info":[{"award-number":["16\/11198-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PADC"},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study reports the performance of the pigment hypericin (HYP)-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system (LCPS) for the treatment of vulvovaginal candidiasis (VVC) in mice. LCPS composed of 40% of ethoxylated and propoxylated cetyl alcohol, 30% of oleic acid and cholesterol (7:1), 30% of a dispersion of 16% poloxamer 407 and 0.05% of HYP (HYP-LCPS) was prepared and characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS) and ex vivo permeation and retention studies across vaginal porcine mucosa were performed. In addition, the antifungal properties of the HYP-LCPS were evaluated in a murine in vivo model; for this, infected C57BL female mice groups were treated with both HYP in solution and HYP-LCPS, and after 6 days colony forming unit (CFU)\/ml count was performed. PLM and SAXS confirmed that HYP-LCPS is a microemulsion situated in boundary transition region confirming its action as an LCPS. When in contact with simulated vaginal fluid, HYP-LCPS became rigid and exhibited maltase crosses and bragg peaks characteristics of lamellar phase. Ex vivo permeation and retention studies showed that HYP-LCPS provides a localized treatment on the superficial layers of porcine vaginal mucosa. HYP-LCPS induced a significant reduction in the number of CFU\/ml in the mice; thus this formulation indicated it is as effective as a commercial dosage form. It was concluded that LCPS maintains the biological activity of HYP and provides an adequate drug delivery system for this lipophilic molecule at the vaginal mucosa, being a promising option in cases of VVC.<\/jats:p>","DOI":"10.1093\/mmy\/myab006","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T01:45:08Z","timestamp":1613612708000},"page":"821-827","source":"Crossref","is-referenced-by-count":14,"title":["<i>In vivo<\/i>\n                    study of hypericin-loaded poloxamer-based mucoadhesive\n                    <i>in situ<\/i>\n                    gelling liquid crystalline precursor system in a mice model of vulvovaginal candidiasis"],"prefix":"10.1093","volume":"59","author":[{"given":"Patricia Rocha","family":"de Ara\u00fajo","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences , 14800\u2013903, Araraquara, S\u00e3o 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Rillo","family":"Sato","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences , 14800\u2013903, Araraquara, S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Fernanda","family":"Rodero","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences , 14800\u2013903, Araraquara, S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Augusto","family":"Oshiro-Junior","sequence":"additional","affiliation":[{"name":"Para\u00edba State University , Campina Grande, Para\u00edba 58429\u2013500,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u00eds Maria","family":"Bauab","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences , 14800\u2013903, 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