{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:26:49Z","timestamp":1787826409747,"version":"build-2784847793"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:label\/>\n                  <jats:p>Serum (1\u21923)-\u03b2-D-glucan (BDG), an pan fungal antigen, is detected in some invasive fungal diseases (IFDs). We compared two commercial kits, the Fungitell assay (FA) (colorimetric) and the Wako assay (WA) (turbidimetric) over a 4-month period to prospectively test 171 patients who mainly had hematological conditions (62%) and experienced episodes (n\u00a0=\u00a0175) of suspected invasive fungal infection. Twenty-three episodes due to BDG-producing fungi were diagnosed (pneumocystosis, n\u00a0=\u00a012; invasive aspergillosis, n\u00a0=\u00a05; candidemia, n\u00a0=\u00a03; invasive fusariosis, n\u00a0=\u00a02; hepato-splenic candidiasis, n\u00a0=\u00a01).<\/jats:p>\n                  <jats:p>Both assays provided similar areas under the curves (AUC\u00a0=\u00a00.9). Using the optimized positivity thresholds (\u2265120 pg\/ml for FA and \u2265 4 pg\/ml for WA), the sensitivity and specificity were 81.8% (CI95: 61.5\u201392.7), 94.8% (90.1\u201397.3) for FA and 81.8% (61.5\u201392.7), 95.4% (90.9\u201397.8) for WA. Negative predictive value was 97.3% (93.3\u201399.0) for both tests. If the manufacturer's positivity threshold (\u226511 pg\/ml) was applied, the WA sensitivity decreased to 50%. Among 71 patients with bacterial infections, 21.1% were FA-positive and 5.6% were WA-positive (p &amp;lt; 10\u22122).<\/jats:p>\n                  <jats:p>The WA performed similarly as compared to the FA with an optimized cutoff value. The WA is a single sample test that is clinically relevant when a prompt therapeutic decision is required.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Lay Summary<\/jats:title>\n                  <jats:p>Serum (1\u21923)-\u03b2-D-glucan testing is dominated by two kits including Fungitell colorimetric assay (FA) and the Wako turbidimetric assay (WA). We compared them prospectively and observed that they both perform similarly when selecting their optimal threshold (\u2265120 pg\/ml for FA and\u00a0\u2265\u00a04 pg\/ml for WA).<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/mmy\/myab016","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T15:13:09Z","timestamp":1614957189000},"page":"882-889","source":"Crossref","is-referenced-by-count":19,"title":["Prospective comparison of (1,3)-beta-D-glucan detection using colorimetric and turbidimetric assays for diagnosing invasive fungal disease"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-3082","authenticated-orcid":false,"given":"Alexandre","family":"Alanio","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"},{"name":"Universit\u00e9 de Paris, 75006 Paris, France"},{"name":"Institut Pasteur, Unit\u00e9 de Mycologie Mol\u00e9culaire, CNRS UMR2000, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maud","family":"Gits-Muselli","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"},{"name":"Universit\u00e9 de Paris, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Guigue","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blandine","family":"Denis","sequence":"additional","affiliation":[{"name":"Service de maladies infectieuses et tropicales, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bergeron","sequence":"additional","affiliation":[{"name":"Service de Pneumologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Touratier","sequence":"additional","affiliation":[{"name":"Pharmacie centrale, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia","family":"Hamane","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-3800","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bretagne","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Lariboisi\u00e8re, Saint-Louis, Fernand Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010 Paris, France"},{"name":"Universit\u00e9 de Paris, 75006 Paris, France"},{"name":"Institut Pasteur, Unit\u00e9 de Mycologie Mol\u00e9culaire, CNRS UMR2000, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,4,20]]},"reference":[{"key":"2021090300022249400_bib1","doi-asserted-by":"crossref","first-page":"e344","DOI":"10.1016\/S1473-3099(17)30304-3","article-title":"Candida and invasive mould diseases in non-neutropenic critically ill 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