{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:26:11Z","timestamp":1781853971323,"version":"3.54.5"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T00:00:00Z","timestamp":1620864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:label\/>\n                  <jats:p>Optimal sensitivity to detect low Pneumocystis loads is of importance to take individual and collective measures to avoid evolution towards Pneumocystis pneumonia and outbreaks in immunocompromised patients. This study compares two qPCR procedures, a new automated RTqPCR using the GeneLEAD VIII extractor\/thermocycler (GLVIII; \u223c2.2\u00a0h workflow) and a previously validated in-house qPCR assays (IH; \u223c5\u00a0h workflow) both targeting mtSSU and mtLSU for detecting P. jirovecii in 213 respiratory samples. GLVIII was found to be more sensitive than IH, detecting eight more specimens. Bland-Altman analysis between the two procedures showed a Cq bias of 1.17\u00a0\u00b1\u00a00.07 in favor of GLVIII.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Lay Summary<\/jats:title>\n                  <jats:p>The fungus Pneumocystis needs to be detected early in respiratory samples to prevent pneumonia in immunocompromised hosts. We evaluated a new commercial RTqPCR on 213 respiratory samples to detect Pneumocystis and found it more sensitive and faster than our routine sensitive in-house qPCR assay.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/mmy\/myab029","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T19:51:38Z","timestamp":1620676298000},"page":"845-848","source":"Crossref","is-referenced-by-count":13,"title":["Increased sensitivity of a new commercial reverse transcriptase-quantitative PCR for the detection of <i>Pneumocystis jirovecii<\/i> in respiratory specimens"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3821-1762","authenticated-orcid":false,"given":"Sarah","family":"Delli\u00e8re","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010, 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0470-6866","authenticated-orcid":false,"given":"Maud","family":"Gits-Muselli","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010, Paris, France"},{"name":"Institut Pasteur, Molecular Mycology Unit, CNRS UMR2000, 75015, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-3800","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bretagne","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), 75010, Paris, France"},{"name":"Institut Pasteur, Molecular Mycology 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