{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:29:42Z","timestamp":1789010982845,"version":"build-2803163510"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["UID\/Multi\/00709\/2019"],"award-info":[{"award-number":["UID\/Multi\/00709\/2019"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["SFRH\/BPD\/124437\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/124437\/2016"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chronic vulvovaginal candidosis results either from reinfection or from the ability of Candida spp. to persist in the vulva and\/or vagina. Persistence is usually associated with increased antifungal (mainly azoles) resistance rates, which can explain treatment failure, and\/or increased expression of virulence factors by Candida spp. The aim of this study was to assess the mechanisms leading to Candida spp persistence, by studying sequential isolates from women with chronic vulvovaginal candidosis, focusing on strains genotypes, azole resistance, and ability to form biofilms along the period of clinical evaluation. The strains were identified at species level by automated analysis of biochemical profiles and molecular typing evaluated by polymorphic DNA analysis. The capacity to form biofilm was assessed with a microtiter plate assay. Fluconazole susceptibility was determined by the microdilution broth assay at both pH 7 (following the recommended guideline) and pH 4.5 (as representative of vaginal pH). We studied samples from 17 clinically recurrent cases. In 53% of the chronic cases there were two or more isolates that had a phylogenetic relationship while the remaining (47%) were caused by different species. In those cases where related strains were involved in recurrence, we verified an increase in MIC at pH 7 and also an increased capacity to form biofilms over time. Significant correlation between these two parameters was observed only in cases caused by C. glabrata, evidencing the importance of these two factors to enhance persistence in the vaginal mucosa for this particular species.<\/jats:p>","DOI":"10.1093\/mmy\/myab048","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T19:13:42Z","timestamp":1629486822000},"page":"1166-1173","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of overtime phenotypic variation of yeasts in chronic vulvovaginal candidosis cases"],"prefix":"10.1093","volume":"59","author":[{"given":"Paula","family":"Faria-Gon\u00e7alves","sequence":"first","affiliation":[{"name":"CICS-UBI: Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior , 6200-506 Covilh\u00e3, Portugal"},{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, University of Beira Interior , 6200-506 Covilh\u00e3, Portugal"},{"name":"FMUMN: Faculty of Medicine, University 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Coimbra, Portugal"},{"name":"FMUC - Faculty of Medicine, University of Coimbra , 3004-504 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Martinez-de-Oliveira","sequence":"additional","affiliation":[{"name":"CICS-UBI: Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior , 6200-506 Covilh\u00e3, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Palmeira-de-Oliveira","sequence":"additional","affiliation":[{"name":"CICS-UBI: Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior , 6200-506 Covilh\u00e3, Portugal"},{"name":"Faculdade de Ci\u00eancias da Sa\u00fade, University of Beira Interior , 6200-506 Covilh\u00e3, Portugal"},{"name":"Labfit-HPRD:Health Products Research and Development Lda , 6200-284 Covilh\u00e3, 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