{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:23:32Z","timestamp":1789698212401,"version":"4.0.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/01390-8"],"award-info":[{"award-number":["2017\/01390-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi of the Paracoccidioides genus, being endemic in Latin America and with the highest number of cases in Brazil. Paracoccidioides spp. release a wide range of molecules, such as enzymes, which may be important for PCM establishment. Here, we identified the 85- and 90-kDa proteins from the supernatants of P. brasiliensis cultures as being an \u03b1-mannosidase. Because the expected mass of this \u03b1-mannosidase is 124.2-kDa, we suggest that the proteins were cleavage products. Indeed, we found an \u03b1-mannosidase activity in the culture supernatants among the excreted\/secreted antigens (ESAg). Moreover, we determined that the enzyme activity was optimal in buffer at pH 5.6, at the temperature of 45\u00baC, and with a concentration of 3\u00a0mM of the substrate p-NP-\u03b1-D-Man. Remarkably, we showed that the gene expression of this \u03b1-mannosidase was higher in yeasts than hyphae in three P. brasiliensis isolates with different virulence degrees that were grown in Ham's F12 synthetic medium for 15 days. But in complex media YPD and Fava Netto, the significantly higher gene expression in yeasts than in hyphae was seen only for the virulent isolate Pb18, but not for intermediate virulence Pb339 and low virulence Pb265 isolates. These results about the high expression of the \u03b1-mannosidase gene in the pathogenic yeast form of P. brasiliensis open perspectives for studying this \u03b1-mannosidase concerning the virulence of P. brasiliensis isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Lay summary<\/jats:title>\n                    <jats:p>Paracoccidioides brasiliensis causes deep mycosis, paracoccidioidomycosis. We determined for the first time the biochemical properties of an \u03b1-mannosidase released by this fungus. We suggest that the enzyme gene expression in the fungus is associated with fungal morphology, stress, and virulence.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/mmy\/myac002","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T15:12:27Z","timestamp":1642777947000},"source":"Crossref","is-referenced-by-count":1,"title":["Biochemical characterization and analysis of gene expression of an \u03b1-mannosidase secreted by\n                    <i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1093","volume":"60","author":[{"given":"Priscila C","family":"Corr\u00eaa","sequence":"first","affiliation":[{"name":"Graduate Program in Basic and Applied Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , 14049-900, Ribeir\u00e3o Preto, SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabr\u00edcio F","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Paulista University , 14024-270, Ribeir\u00e3o Preto, SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo V","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Philosophy , Sciences and Letters at Ribeir\u00e3o Preto, , 14040-901, Ribeir\u00e3o Preto, SP,","place":["Brazil"]},{"name":"University of S\u00e3o Paulo , Sciences and Letters at Ribeir\u00e3o Preto, , 14040-901, Ribeir\u00e3o Preto, SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taise N","family":"Landgraf","sequence":"additional","affiliation":[{"name":"Anhanguera Educational, Leme , 13614-370, SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0148-0133","authenticated-orcid":false,"given":"Ademilson","family":"Panunto-Castelo","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Philosophy , Sciences and 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