{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:21:29Z","timestamp":1792624889593,"version":"4.5.0"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T00:00:00Z","timestamp":1641945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>We reviewed the performance of a panfungal ITS-2 PCR and Sanger sequencing assay performed on 88 FFPE specimens at The Hospital for Sick Children (Toronto, Canada) in 2019. A potential fungal pathogen was identified by ITS PCR in 62.7 and 2.9% of positive and negative direct slide examination of tissue specimens, respectively. ITS amplicons were detected in 87\/88 specimens, with 53\/88 (60.2%) considered as \u2018positive-contaminants\u2019 and 34\/88 (38.6%) as \u2018positive-potential pathogen\u2019 upon sequencing. Potential pathogens included Blastomyces dermatitidis (17.1%), Cryptococcus neoformans (17.1%), Histoplasma capsulatum (14.3%) and Mucormycetes (11.4%). Laboratories should only perform ITS PCR on FFPE tissues if fungal elements have been confirmed on histopathology slides.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Lay summary<\/jats:title>\n                    <jats:p>In this study, we examined how well a DNA-based test could detect DNA from fungi in archived human biopsy tissues. The best performance was achieved if fungi were seen in the tissue under a microscope before being tested. Our results indicate that we should only use this test if these conditions are met.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/mmy\/myac004","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T15:10:20Z","timestamp":1641827420000},"source":"Crossref","is-referenced-by-count":15,"title":["Assessment of panfungal PCR performance with formalin-fixed paraffin-embedded tissue specimens"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7160-304X","authenticated-orcid":false,"given":"Shawn T","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne C W","family":"Yau","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada"},{"name":"Department of Pediatric Laboratory Medicine, Division of Microbiology, Hospital for Sick Children, Toronto, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Campigotto","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada"},{"name":"Department of Pediatric Laboratory Medicine, Division of Microbiology, Hospital for Sick Children, Toronto, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Gharabaghi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Laboratory Medicine, Division of Microbiology, Hospital for Sick Children, Toronto, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan E","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada"},{"name":"Department of Pediatric Laboratory Medicine, Division of Microbiology, Hospital for Sick Children, Toronto, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal","family":"Tadros","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada"},{"name":"Department of Pediatric Laboratory Medicine, Division of Microbiology, Hospital for Sick Children, Toronto, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,1,12]]},"reference":[{"key":"2022030309451847800_bib1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1053\/j.semdp.2019.04.010","article-title":"Emerging and re-emerging fungal infections","volume":"36","author":"Lockhart","year":"2019","journal-title":"Semin Diagnostic 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