{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:46:33Z","timestamp":1761129993285,"version":"3.41.2"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:label\/>\n                  <jats:p>PCR-based methods applied to various body fluids emerged in recent years as a promising approach for the diagnosis of mucormycosis. In this study, we set up and assess the value of a qPCR to detect a wide variety of Mucorales species in a single tube. A pair of degenerated primers targeting the rDNA operon was used in a qPCR utilizing an intercalating fluorescent dye. Analytical assessment, using a wide variety of both Mucorales strains (8 genera, 11 species) and non-Mucorales strains (9 genera, 14 species), showed 100% sensitivity and specificity rates with a limit of detection at 3 rDNA copy\/qPCR reaction. Subsequently, 364 clinical specimens from 166 at-risk patients were prospectively tested with the assay. All the seven patients classified as proven\/probable mucormycosis using the EORTC-MSG criteria had a positive qPCR as well as a patient with a proven uncharacterized invasive mold infection. In addition, three out of seven patients with possible mold invasive infections had at least one positive qPCR test. Sensitivity was calculated between 73.33 and 100% and specificity between 98.10 and 100%. The qPCR method proposed showed excellent performances and would be an important adjunctive tool for the difficult diagnosis of mucormycosis diagnosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Lay abstract<\/jats:title>\n                  <jats:p>qPCR-based diagnosis is the most reliable approach for mucormycosis. We set up a pan-Mucorales qPCR able to detect in a single reaction not less than 11 different species. Both analytical and clinical performances support its use in the clinical setting.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/mmy\/myac015","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T20:10:52Z","timestamp":1645128652000},"source":"Crossref","is-referenced-by-count":14,"title":["Diagnosis of mucormycosis using an intercalating dye-based quantitative PCR"],"prefix":"10.1093","volume":"60","author":[{"given":"Jeanne","family":"Bigot","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Centre de Recherche Saint-Antoine, CRSA, APHP, H\u00f4pital Saint Antoine, Laboratoire de Parasitologie Mycologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Godmer","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, 75013, Paris, France"},{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Saint Antoine, D\u00e9partement de Bact\u00e9riologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lysa","family":"Prudent\u00e9","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Saint Antoine, Laboratoire de Parasitologie Mycologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"Angebault","sequence":"additional","affiliation":[{"name":"Univ-Paris-Est-Cr\u00e9teil (UPEC), Facult\u00e9 de Sant\u00e9, EA DYNAMiC 7380 ; APHP, CHU Henri Mondor, Unit\u00e9 de Parasitologie Mycologie, D\u00e9partement de Pr\u00e9vention, Diagnostic et Traitement des Infections, 94010 Cr\u00e9teil, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eolia","family":"Brissot","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM U938, APHP, H\u00f4pital Saint Antoine, Service d'H\u00e9matologie et Th\u00e9rapie Cellulaire, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naike","family":"Bige","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Saint Antoine, Service de R\u00e9animation M\u00e9dicale, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"Voiriot","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Tenon, Service de M\u00e9decine Intensive et R\u00e9animation, 75020 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre-Louis","family":"Leger","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Trousseau, Service de R\u00e9animation P\u00e9diatrique, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille","family":"Petit-Hoang","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Tenon, Service des Urgences N\u00e9phrologiques et Transplantation R\u00e9nale, 75020, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Atallah","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Tenon, Service de'Oto-Rhino-Laryngologie, 75020 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elodie","family":"Gouache","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Trousseau, D\u00e9partement d'H\u00e9matologie et d'Oncologie P\u00e9diatrique, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaye","family":"Senghor","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, APHP, H\u00f4pital Saint Antoine, Laboratoire de Parasitologie Mycologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Valot","sequence":"additional","affiliation":[{"name":"CHU Dijon, Plateforme de Biologie Hospitalo-Universitaire, Laboratoire de Parasitologie Mycologie, 21070 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Hennequin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Centre de Recherche Saint-Antoine, CRSA, APHP, H\u00f4pital Saint Antoine, Laboratoire de Parasitologie Mycologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9782-3577","authenticated-orcid":false,"given":"Juliette","family":"Guitard","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Centre de Recherche Saint-Antoine, CRSA, APHP, H\u00f4pital Saint Antoine, Laboratoire de Parasitologie Mycologie, 75012 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"2022031210502158000_bib1","doi-asserted-by":"crossref","first-page":"e405","DOI":"10.1016\/S1473-3099(19)30312-3","article-title":"Global guideline for the diagnosis and management of mucormycosis: an initiative of the European Confederation of Medical Mycology in cooperation with the Mycoses Study Group Education and Research Consortium","volume":"19","author":"Cornely","year":"2019","journal-title":"Lancet Infect Dis"},{"key":"2022031210502158000_bib2","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1086\/651263","article-title":"Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients, 2001-2006: overview of the Transplant-Associated Infection Surveillance Network (TRANSNET) database","volume":"50","author":"Kontoyiannis","year":"2010","journal-title":"Clin Infect Dis"},{"key":"2022031210502158000_bib3","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.3201\/eid1710.110087","article-title":"Invasive non-Aspergillus mold infections in transplant recipients, United States, 2001-2006","volume":"17","author":"Park","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"2022031210502158000_bib4","first-page":"e2021271","article-title":"Rising concerns of mucormycosis (Zygomycosis) among COVID-19 patients; 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