{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:15:53Z","timestamp":1789150553215,"version":"build-2803163510"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006280","name":"Ministerio de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["2019-04838"],"award-info":[{"award-number":["2019-04838"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Candida auris is considered a public health problem because of its resistance and its tendency to cause nosocomial outbreaks. CHROMagarTMCandida Plus has recently been marketed as capable of presumptively identifying C. auris. The objective of this work was to analyze the ability of this new chromogenic medium to differentiate C. auris from other members of the C. haemulonii complex and from other yeasts commonly isolated in clinical practice. A collection of 220 strains including species of the C. haemulonii (n\u00a0=\u00a083) and C. parapsilosis (n\u00a0=\u00a080) complexes was studied. The strains were identified by molecular methods and cultured as individual or as mixed aqueous inoculum on CHROMagarTMCandida Plus plates. Colony morphotypes were evaluated at five time points. CHROMagarTMCandida Plus was a helpful tool for presumptive identification for C. auris. Better reading results were obtained after 48 h of incubation at 35\u00b0C. It is able to easily differentiate C. auris from other closely related species of the C. haemulonii complex and other yeasts. This chromogenic medium would be also useful as screening and surveillance tool for C. auris colonization. However, we demonstrated that it would be a possible misidentification of C. parapsilosis as C. auris (44.3% showed similar morphotypes). To reduce false positives when it is used in a context of a C. auris outbreak, we propose to supplement the chromogenic medium with 8 \u03bcg\/ml fluconazole. This modified medium was tested, and it clearly differentiate C. parapsilosis from C. auris.<\/jats:p>","DOI":"10.1093\/mmy\/myac062","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T21:37:22Z","timestamp":1665265042000},"source":"Crossref","is-referenced-by-count":19,"title":["<i>Candida auris<\/i>\n                    and some\n                    <i>Candida parapsilosis<\/i>\n                    strains exhibit similar characteristics on CHROMagarTM\n                    <i>Candida<\/i>\n                    Plus"],"prefix":"10.1093","volume":"60","author":[{"given":"Natalia","family":"Sasoni","sequence":"first","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica, Universidad Nacional del Litoral, Ciudad Universitaria , CP 3000 Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melani","family":"Maidana","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica, Universidad Nacional del Litoral, Ciudad Universitaria , CP 3000 Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Gabriela","family":"Latorre-Rapela","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica, Universidad Nacional del Litoral, Ciudad Universitaria , CP 3000 Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraya","family":"Morales-Lopez","sequence":"additional","affiliation":[{"name":"Grupo CINBIOS, Programa de Microbiolog\u00eda, Universidad Popular del Cesar , 200002 Valledupar, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira","family":"Berrio","sequence":"additional","affiliation":[{"name":"Hospital general de Medellin `Luz Castro de Guti\u00e9rrez' ESE. Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas , 050015 Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Gamarra","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica, Universidad Nacional del Litoral, Ciudad Universitaria , CP 3000 Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-4650","authenticated-orcid":false,"given":"Guillermo","family":"Garcia-Effron","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica, Universidad Nacional del Litoral, Ciudad Universitaria , CP 3000 Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET), CP 3000 Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,8]]},"reference":[{"key":"2022101214375404000_bib1","doi-asserted-by":"crossref","first-page":"ofab471","DOI":"10.1093\/ofid\/ofab471","article-title":"Fungal Infection Network of Switzerland (FUNGINOS). 2021. Trends of the epidemiology of candidemia in Switzerland: a 15-year FUNGINOS survey","volume":"8","author":"Adam","year":"2021","journal-title":"Open forum Infect Dis"},{"key":"2022101214375404000_bib2","doi-asserted-by":"crossref","first-page":"e59373","DOI":"10.1371\/journal.pone.0059373","article-title":"Epidemiology of candidemia in Latin America: a laboratory-based survey","volume":"8","author":"Nucci","year":"2013","journal-title":"PLoS One"},{"key":"2022101214375404000_bib3","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1056\/NEJMoa1801550","article-title":"Changes in prevalence of health care\u2013associated infections in U.S. hospitals","volume":"379","author":"Magill","year":"2018","journal-title":"N Engl J Med"},{"key":"2022101214375404000_bib4","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s10096-013-2027-1","article-title":"Multidrug-resistant endemic clonal strain of Candida auris in India","volume":"33","author":"Chowdhary","year":"2014","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2022101214375404000_bib5","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3201\/eid2302.161486","article-title":"Multidrug-resistant Candida haemulonii and C. auris, Tel Aviv, Israel","volume":"23","author":"Ben-Ami","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"2022101214375404000_bib6","doi-asserted-by":"crossref","first-page":"561","DOI":"10.3201\/eid2203.151610","article-title":"Candida haemulonii complex species, Brazil, January 2010-march 2015","volume":"22","author":"de Almeida","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"2022101214375404000_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/emi.2016.49","article-title":"Candida haemulonii species complex: an emerging species in India and its genetic diversity assessed with multilocus sequence and amplified fragment-length polymorphism analyses","volume":"5","author":"Kumar","year":"2016","journal-title":"Emerg Microbes Infect"},{"key":"2022101214375404000_bib8","doi-asserted-by":"crossref","first-page":"e57","DOI":"10.1086\/597108","article-title":"Candida haemulonii and closely related species at 5 university hospitals in Korea: identification, antifungal susceptibility, and clinical features","volume":"48","author":"Kim","year":"2009","journal-title":"Clin Infect Dis"},{"key":"2022101214375404000_bib9","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1128\/JCM.02202-16","article-title":"Can multidrug-resistant candidaauris be reliably identified in clinical microbiology laboratories?","volume":"55","author":"Mizusawa","year":"2017","journal-title":"J Clin Microbiol"},{"key":"2022101214375404000_bib10","doi-asserted-by":"crossref","first-page":"e753","DOI":"10.1093\/cid\/ciaa1462","article-title":"Factors associated with Candida auris colonization and transmission in skilled nursing facilities with ventilator units, New York, 2016-2018","volume":"72","author":"Rossow","year":"2021","journal-title":"Clin Infect Dis"},{"key":"2022101214375404000_bib11"},{"key":"2022101214375404000_bib12"},{"key":"2022101214375404000_bib13","doi-asserted-by":"crossref","first-page":"1900730","DOI":"10.2807\/1560-7917.ES.2021.26.8.1900730","article-title":"Screening for Candida auris in patients admitted to eight intensive care units in England, 2017 to 2018","volume":"26:","author":"Sharp","year":"2021","journal-title":"Euro Surveill"},{"key":"2022101214375404000_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/antibiotics9110778","article-title":"Candida auris: an overview of how to screen, detect, test and control this emerging pathogen","volume":"9","author":"Fasciana","year":"2020","journal-title":"Antibiotics"},{"key":"2022101214375404000_bib15","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.3389\/fmicb.2019.01918","article-title":"Identification of drug resistant Candida auris","volume":"10","author":"Kordalewska","year":"2019","journal-title":"Front Microbiol"},{"key":"2022101214375404000_bib16","doi-asserted-by":"crossref","first-page":"e01337","DOI":"10.1128\/JCM.01337-18","article-title":"Direct detection of emergent fungal pathogen Candida auris in clinical skin swabs by SYBR green-based quantitative PCR assay","volume":"56:","author":"Sexton","year":"2018","journal-title":"J Clin Microbiol"},{"key":"2022101214375404000_bib17","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.riam.2018.01.003","article-title":"Single-tube classical PCR for Candida auris and Candida haemulonii identification","volume":"35","author":"Theill","year":"2018","journal-title":"Rev Iberoam Micol"},{"key":"2022101214375404000_bib18","doi-asserted-by":"crossref","first-page":"115168","DOI":"10.1016\/j.diagmicrobio.2020.115168","article-title":"Evaluation of a novel chromogenic medium for Candida spp. identification and comparison with CHROMagarTMCandida for the detection of Candida auris in surveillance samples","volume":"98","author":"Mulet Bayona","year":"2020","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2022101214375404000_bib19","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1093\/mmy\/myaa049","article-title":"CHROMagarTM Candida plus: a novel chromogenic agar that permits the rapid identification of Candida auris","volume":"59","author":"Borman","year":"2021","journal-title":"Med Mycol"},{"key":"2022101214375404000_bib20","doi-asserted-by":"crossref","first-page":"e03220","DOI":"10.1128\/JCM.03220-20","article-title":"Performance of two novel chromogenic media for the identification of multidrug-resistant Candida auris compared with other commercially available formulations","volume":"59","author":"De Jong","year":"2021","journal-title":"J Clin Microbiol"},{"key":"2022101214375404000_bib21","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12866-017-0958-x","article-title":"Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)","volume":"17","author":"Badotti","year":"2017","journal-title":"BMC Microbiol"},{"key":"2022101214375404000_bib22","first-page":"315","article-title":"Amplificaion and direct sequencing of fungal ribosomal RNA genes for phylogenetics","volume":"2","author":"Kuiper-Goodman","year":"1990","journal-title":"Biomed Environ Sci"},{"key":"2022101214375404000_bib23","doi-asserted-by":"crossref","first-page":"6241","DOI":"10.1073\/pnas.1117018109","article-title":"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi","volume":"109","author":"Schoch","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022101214375404000_bib24","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1093\/jac\/dkz178","article-title":"Antifungal activity and killing kinetics of anidulafungin, caspofungin and amphotericin B against Candida auris","volume":"74","author":"Dudiuk","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"2022101214375404000_bib25","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1128\/jcm.32.8.1923-1929.1994","article-title":"CHROMagar candida, a new differential isolation medium for presumptive identification of clinically important Candida species","volume":"32","author":"Odds","year":"1994","journal-title":"J Clin Microbiol"},{"key":"2022101214375404000_bib26","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1093\/cid\/ciw691","article-title":"Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses","volume":"64","author":"Lockhart","year":"2017","journal-title":"Clin Infect Dis"},{"key":"2022101214375404000_bib27","doi-asserted-by":"crossref","first-page":"e0223433","DOI":"10.1371\/journal.pone.0223433","article-title":"Clade II Candida auris possess genomic structural variations related to an ancestral strain","volume":"14","author":"Sekizuka","year":"2019","journal-title":"PLoS One"},{"key":"2022101214375404000_bib28","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1128\/AAC.04613-14","article-title":"Molecular mechanisms of fluconazole resistance in Candida parapsilosis isolates from a U.S. surveillance system","volume":"59","author":"Grossman","year":"2015","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Medical Mycology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/mmy\/advance-article-pdf\/doi\/10.1093\/mmy\/myac062\/46395975\/myac062.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mmy\/article-pdf\/60\/10\/myac062\/46459895\/myac062.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mmy\/article-pdf\/60\/10\/myac062\/46459895\/myac062.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T14:25:16Z","timestamp":1665584716000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mmy\/article\/doi\/10.1093\/mmy\/myac062\/6754337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10,12]]}},"URL":"https:\/\/doi.org\/10.1093\/mmy\/myac062","relation":{},"ISSN":["1369-3786","1460-2709"],"issn-type":[{"value":"1369-3786","type":"print"},{"value":"1460-2709","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,10]]},"published":{"date-parts":[[2022,10]]},"article-number":"myac062"}}