{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:20:30Z","timestamp":1792790430052,"version":"4.5.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,18]],"date-time":"2023-02-18T00:00:00Z","timestamp":1676678400000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Foundation for Research Support of the State of S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/08419-7"],"award-info":[{"award-number":["2019\/08419-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Foundation for Research Support of the State of S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/08642-8"],"award-info":[{"award-number":["2019\/08642-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Trichosporonosis corresponds to a systemic fungal disease that leads to high mortality rates and is frequently associated with medical devices. It affects immunosuppressed patients in particular and is strongly linked to acquired human immunodeficiency, organ and tissue transplants, and malignant hematologic diseases such as leukemia and lymphomas. Trichosporon infections have been increasingly reported worldwide; however, little information is available either about their characteristics or the causative microorganism. Thus, the aims of the present study were: to investigate 59 yeasts of the genus Trichosporon by verifying the biofilm formation capacity of isolates; to analyze the susceptibility patterns of planktonic cells against the antifungals fluconazole, itraconazole, amphotericin-B, voriconazole, and caspofungin by comparing European Committee for Antimicrobial Susceptibility Testing (EUCAST) broth microdilution technique with the commercial method Etest; and to assess the susceptibility patterns of biofilm cells (sessile) against the same antifungals through broth microdilution. The ability to form biofilm on the surface of polystyrene plates was noted for all isolates, and 54.3% of samples were considered strong producers. Comparison between the antifungal susceptibility techniques evidenced that Etest showed higher and discordant minimum inhibitory concentrations (MICs) from those obtained by the microdilution method, especially for fluconazole, itraconazole, and caspofungin. Considering the susceptibility of biofilms, most species had high MIC50 and MIC90 against the tested antifungals, showing 4-to-66-fold higher concentrations for amphotericin B and 2-to-33-fold greater concentrations for caspofungin. These results highlight the importance of further studies with Trichosporon spp. for comparison between laboratory findings and in vivo response, considering both the susceptibility tests and the behavior of biofilm cells against drugs.<\/jats:p>","DOI":"10.1093\/mmy\/myad022","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T17:15:55Z","timestamp":1676481355000},"source":"Crossref","is-referenced-by-count":6,"title":["Aspects related to biofilm production and antifungal susceptibility of clinically relevant yeasts of the genus\n                    <i>Trichosporon<\/i>"],"prefix":"10.1093","volume":"61","author":[{"given":"Bruna Rossini","family":"Lara","sequence":"first","affiliation":[{"name":"Instituto de Bioci\u00eancias, Universidade Estadual Paulista (UNESP) , Botucatu , S\u00e3o Paulo ,","place":["Brazil"]},{"name":"Instituto Adolfo Lutz (IAL)\u2014CLR II, N\u00facleo de Ci\u00eancias Biom\u00e9dicas, Bauru , S\u00e3o Paulo 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Garcia","family":"Garces","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Universidade Estadual Paulista (UNESP) , Botucatu , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Volpe","family":"Arnoni","sequence":"additional","affiliation":[{"name":"Irmandade da Santa Casa de Miseric\u00f3rdia de S\u00e3o Paulo, Servi\u00e7o de Controle de Infec\u00e7\u00e3o Hospitalar , S\u00e3o Paulo, SP,","place":["Brazil"]},{"name":"Hospital Infantil Darcy Vargas , S\u00e3o Paulo , SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f4nica","family":"Silveira","sequence":"additional","affiliation":[{"name":"Hospital Estadual Bauru (HEB) , Bauru, SP,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Mazo F\u00e1vero","family":"Gimenes","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical, Universidade de 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