{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:58:53Z","timestamp":1759229933457,"version":"3.41.2"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["#304138\/2019-3"],"award-info":[{"award-number":["#304138\/2019-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["CEECIND\/01070\/2018"],"award-info":[{"award-number":["CEECIND\/01070\/2018"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The classical dermatophytes diagnosis is based on mycological culture and microscopy observation both human and animal hair, skin, and nail samples. The aim of this work was to develop the new in-house real-time PCR with pan-dematophyte reaction for detection and identification of the main dermatophytes directly from hair samples, providing a simple and rapid diagnosis of dermatophytosis in dogs and cats.<\/jats:p>\n               <jats:p>An in-house SYBR-Green real-time PCR was designed and used for detecting a DNA fragment encoding chitin synthase 1 (CHS1). A total of 287 samples were processed by culture, microscopic examination with KOH 10%, and real-time PCR (qPCR) analysis. Melting curve analysis of the CHS1 fragment revealed to be reproducible, showing a single distinct peak for each species of dermatophyte, namely Trichophyton mentagrophytes, T. verrucosum, Microsporum canis, and Nannizzia gypsea (formerly M. gypseum). Then, out of the 287 clinically suspected cases of dermatophytosis, 50% were positive for dermatophytes by qPCR, 44% by mycological culture, and 25% by microscopic examination. Microsporum canis was identified in 117 samples tested by culture and 134 samples tested by qPCR, followed by N. gypsea in 5 samples (either tested by culture or qPCR) and T. mentagrophytes detected in 4 and 5 samples when tested by culture or qPCR, respectively. Overall, qPCR allowed the diagnosis of dermatophytosis in clinical samples. The results suggest this newly proposed in-house real-time PCR assay can be used as alternative diagnosis and rapid identification of dermatophytes frequently associated to clinical hair samples of dogs and cats.<\/jats:p>","DOI":"10.1093\/mmy\/myad047","type":"journal-article","created":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T10:10:34Z","timestamp":1682849434000},"source":"Crossref","is-referenced-by-count":3,"title":["Molecular detection and species identification of dermatophytes by SYBR-Green real-time PCR in-house methodology using hair samples obtained from dogs and\u00a0cats"],"prefix":"10.1093","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1895-6708","authenticated-orcid":false,"given":"Andr\u00e9ia","family":"Spanamberg","sequence":"first","affiliation":[{"name":"Setor de Micologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre, RS , Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias (PPGCV), UFRGS , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Ravazzolo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia e Biologia Molecular, Faculdade de Veterin\u00e1ria, (UFRGS) , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Araujo","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade , Porto , Portugal"},{"name":"INEB\u2014Instituto Nacional de Engenharia Biom\u00e9dica, Universidade do Porto , Porto , Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia","family":"Tomazi","sequence":"additional","affiliation":[{"name":"Setor de Micologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre, RS , Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias (PPGCV), UFRGS , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Setor de Micologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre, RS , Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias (PPGCV), UFRGS , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laerte","family":"Ferreiro","sequence":"additional","affiliation":[{"name":"Setor de Micologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre, RS , Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias (PPGCV), UFRGS , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"2023051121360654400_bib1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s11046-008-9102-7","article-title":"Dermatophytoses in animals","volume":"166","author":"Chermette","year":"2008","journal-title":"Mycopathologia"},{"key":"2023051121360654400_bib2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3390\/vetsci9010021","article-title":"Prevalence of Microsporum canis from pet cats in small animal hospitals, Chiang Mai, Thailand","volume":"9","author":"Chupia","year":"2022","journal-title":"Vet Sci"},{"key":"2023051121360654400_bib3","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1590\/abd1806-4841.20197491","article-title":"Occurrence of dermatophytoses in patients from the Sistema \u00danico de 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