{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:06:09Z","timestamp":1793253969235,"version":"4.5.0"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T00:00:00Z","timestamp":1706313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To estimate the diagnostic performance of Mucorales polymerase chain reaction (PCR) in Bronchoalveolar lavage fluid (BALF) in routine practice. This was a single-center retrospective study including all consecutive patients\u00a0&amp;gt;18 years who underwent Mucorales PCR assay in BALF between January 2021 and May 2022. Index testing was prospectively performed using the MycoGENIE Aspergillus spp.\u2013Mucorales spp. PCR. The reference was the diagnosis of pulmonary mucormycosis by the Adjudication Committee. Mucorales PCR in BALF was performed for 938 patients and was positive for 21 of 938 (2.2%). Eleven pulmonary mucormycosis (including one disseminated) were diagnosed. Among them, one (9.1%) was classified as proven mucormycosis, three (27.3%) as probable, and seven (63.6%) as possible according to the EORTC\/MSGERC 2019 criteria. The main host factor was hematological malignancy (10 of 11, 90.9%). Mucorales PCR was positive in serum for eight patients (72.7%). Three patients had positive PCR in BALF, but negative in serum. The mean cycle threshold value was significantly lower in mucormycosis than false-positive cases. Sensitivity was 72.7% (95% confidence interval [CI], 43.4\u201390.3%), and specificity was 98.6% (95% CI, 97.6\u201399.2%). The positive and negative predictive values were 38.1% (95% CI, 20.8\u201359.1%) and 99.7% (95% CI, 99.1\u201399.9%), respectively. Mucorales PCR in BALF showed good diagnostic performance for mucormycosis, particularly in combination with serum PCR. A positive result should be interpreted with caution, given the possibility of carriage in the airway. However, its high negative predictive value and specificity suggest the utility of Mucorales PCR in BALF in the diagnosis of pulmonary mucormycosis.<\/jats:p>","DOI":"10.1093\/mmy\/myae006","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T13:11:04Z","timestamp":1706533864000},"source":"Crossref","is-referenced-by-count":18,"title":["Performance of Mucorales spp. qPCR in bronchoalveolar lavage fluid for the diagnosis of pulmonary mucormycosis"],"prefix":"10.1093","volume":"62","author":[{"given":"Xavier","family":"Brousse","sequence":"first","affiliation":[{"name":"Service des maladies infectieuses & tropicales, H\u00f4pital Pellegrin, CHU de Bordeaux , Bordeaux , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Imbert","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Pellegrin, CHU de Bordeaux , Bordeaux , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nah\u00e9ma","family":"Issa","sequence":"additional","affiliation":[{"name":"M\u00e9decine intensive r\u00e9animation, H\u00f4pital Saint-Andr\u00e9, CHU de Bordeaux , Bordeaux , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard","family":"Forcade","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie et de th\u00e9rapie cellulaire, H\u00f4pital Haut-L\u00e9v\u00eaque CHU de Bordeaux , Pessac , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Faure","sequence":"additional","affiliation":[{"name":"Service de traitement de l'insuffisance cardiaque, de l'HTAP et de suivi des greffes et assistances cardiaques, H\u00f4pital Haut-L\u00e9v\u00eaque, CHU de Bordeaux , Pessac , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Chambord","sequence":"additional","affiliation":[{"name":"Pharmacie \u00e0 usage int\u00e9rieure, H\u00f4pital Haut-L\u00e9v\u00eaque, CHU de Bordeaux , Pessac , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanta","family":"Ramaroson","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Coordination et d'Analyse de l'Information M\u00e9dicale \u2013 D\u00e9partement d'Information M\u00e9dicale (UCAIM-DIM), CHU de Bordeaux , Bordeaux , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Service de N\u00e9phrologie, Transplantation Dialyse, Aph\u00e9r\u00e8ses, H\u00f4pital Pellegrin , Bordeaux , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Yves","family":"Dumas","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie et de th\u00e9rapie cellulaire, H\u00f4pital Haut-L\u00e9v\u00eaque CHU de Bordeaux , Pessac , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5445-9122","authenticated-orcid":false,"given":"Elodie","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Service de Pneumologie, H\u00f4pital Haut-L\u00e9v\u00eaque, CHU de Bordeaux , Pessac , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,30]]},"reference":[{"key":"2024021702092963900_bib1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3390\/jof5010026","article-title":"Global epidemiology of mucormycosis","volume":"5","author":"Prakash","year":"2019","journal-title":"J Fungi (Basel)"},{"key":"2024021702092963900_bib2","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.3201\/eid1509.090334","article-title":"Increasing incidence of zygomycosis (mucormycosis), France, 1997-2006","volume":"15","author":"Bitar","year":"2009","journal-title":"Emerg Infect. 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