{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:47:46Z","timestamp":1778575666541,"version":"3.51.4"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003074","name":"ANPCyT","doi-asserted-by":"publisher","award":["PICT-2021-I-A-00557"],"award-info":[{"award-number":["PICT-2021-I-A-00557"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Definitive diagnosis of histoplasmosis relies on culture and\/or cytology\/histopathology; however, these procedures have limited sensitivity and cultures are time-consuming. Antibodies detection by immunodiffusion has low sensitivity in immunocompromised individuals and uses histoplasmin (HMN), a crude antigenic extract, as reagent. Novel protein antigen candidates have been recently identified and produced by DNA-recombinant techniques to obtain standardized and specific reagents for diagnosing histoplasmosis.<\/jats:p><jats:p>To compare the analytical performance of novel enzyme-linked immunosorbent assays (ELISAs) for antibodies testing for diagnosing histoplasmosis using different Histoplasma capsulatum antigens as reagents.<\/jats:p><jats:p>The H. capsulatum 100\u00a0kDa protein (Hcp100), the M antigen and its immunoreactive fragment F1 were produced by DNA-recombinant techniques. Galactomannan was purified from both the yeast and mycelial cell walls (yGM and mGM, respectively). The analytical performance of the ELISA tests for the serological detection of antibodies against these antigens was evaluated and compared with those obtained using HMN as reagent.<\/jats:p><jats:p>Antibodies detection by the Hcp100 ELISA demonstrated 90.0% sensitivity and 92.0% specificity, versus 43.3% sensitivity and 95.0% specificity of the M ELISA, 33.3% sensitivity and 84.0% specificity of the F1 ELISA, 96.7% sensitivity and 94.0% specificity of the yGM ELISA, 83.3% sensitivity and 88.0% specificity of the mGM ELISA, and 70.0% sensitivity and 86.0% specificity for the HMN ELISA.<\/jats:p><jats:p>In summary, Hcp100 is proposed as the most promising candidate for the serodiagnosis of histoplasmosis. The primary immunoreactive element in HMN proved to be GM rather than the M antigen. Nevertheless, a higher incidence of cross-reactions was noted with GM compared to M.<\/jats:p>","DOI":"10.1093\/mmy\/myae023","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:56:45Z","timestamp":1710377805000},"source":"Crossref","is-referenced-by-count":2,"title":["<i>Histoplasma<\/i>antigens as novel players for the development of new enzyme immunoassays for the serodiagnosis of histoplasmosis: A comparative study of their analytical performance"],"prefix":"10.1093","volume":"62","author":[{"given":"Carolina","family":"Rodr\u00edguez\u00a0Laboccetta","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires. Facultad de Farmacia y Bioqu\u00edmica. Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC). Buenos Aires , Argentina"},{"name":"Universidad de Buenos Aires. CONICET. Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM). Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor J","family":"Brice\u00f1o\u00a0Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Facultad de Farmacia y Bioqu\u00edmica. Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC). Buenos Aires , Argentina"},{"name":"Universidad de Buenos Aires. CONICET. Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM). Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agust\u00edn","family":"Videla\u00a0Garrido","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. CONICET. Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM). Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gladys B","family":"Posse","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda. Hospital Nacional Profesor Alejandro Posadas. Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4467-996X","authenticated-orcid":false,"given":"Mar\u00eda L","family":"Cuestas","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. CONICET. Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM). Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8178-4764","authenticated-orcid":false,"given":"Alejandro D","family":"Nusblat","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Facultad de Farmacia y Bioqu\u00edmica. Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC). Buenos Aires , Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"2024041116390910900_bib1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00027-06","article-title":"Histoplasmosis: A clinical and laboratory update","volume":"20","author":"Kauffman","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"2024041116390910900_bib2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1007\/s11046-020-00431-2","article-title":"Re-drawing the maps for endemic mycoses","volume":"185","author":"Ashraf","year":"2020","journal-title":"Mycopathologia"},{"key":"2024041116390910900_bib3","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s11046-021-00588-4","article-title":"Pulmonary histoplasmosis","volume":"186","author":"Tob\u00f3n","year":"2021","journal-title":"Mycopathologia"},{"key":"2024041116390910900_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01376-14","article-title":"Comparison of phylogenetically distinct Histoplasma strains reveals evolutionarily divergent virulence strategies","volume":"5","author":"Sep\u00falveda","year":"2014","journal-title":"mBio"},{"key":"2024041116390910900_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/msphere.00742-20","article-title":"Biodiverse histoplasma species elicit distinct patterns of pulmonary inflammation following sublethal infection","volume-title":"mSphere","author":"Jones","year":"2020"},{"key":"2024041116390910900_bib6","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.mmcr.2017.07.004","article-title":"The important role of co-infections in patients with AIDS and progressive disseminated histoplasmosis (PDH): A cohort from Colombia","volume":"19","author":"Caceres","year":"2018","journal-title":"Med Mycol Case Rep"},{"key":"2024041116390910900_bib7","first-page":"157","article-title":"Histoplasma capsulatum: mechanisms for pathogenesis","volume":"422","author":"Mittal","year":"2019","journal-title":"Curr Top Microbiol Immunol"},{"key":"2024041116390910900_bib8","doi-asserted-by":"crossref","first-page":"e367","DOI":"10.1016\/S1473-3099(17)30306-7","article-title":"Neglected endemic mycoses","volume":"17","author":"Queiroz-Telles","year":"2017","journal-title":"Lancet Infect Dis"},{"key":"2024041116390910900_bib9","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1128\/JCM.02430-16","article-title":"Laboratory diagnostics for histoplasmosis","volume":"55","author":"Azar","year":"2017","journal-title":"J Clin Microbiol"},{"key":"2024041116390910900_bib10","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1007\/s00253-021-11170-9","article-title":"Diagnosis of histoplasmosis: current status and perspectives","volume":"105","author":"Toscanini","year":"2021","journal-title":"Appl Microbiol Biotechnol"},{"key":"2024041116390910900_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/jof5030076","article-title":"Diagnosis of progressive disseminated histoplasmosis in advanced HIV: A meta-analysis of assay analytical performance","volume":"5","author":"C\u00e1ceres","year":"2019","journal-title":"J Fungi"},{"key":"2024041116390910900_bib12","doi-asserted-by":"crossref","first-page":"105981","DOI":"10.1016\/j.mimet.2020.105981","article-title":"Roschman-Gonz\u00e1lez A. 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