{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:12:34Z","timestamp":1792635154803,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["405653\/2021-2"],"award-info":[{"award-number":["405653\/2021-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"FIOCRUZ","doi-asserted-by":"publisher","award":["23\/2551-0000503-4"],"award-info":[{"award-number":["23\/2551-0000503-4"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Zoonotic sporotrichosis caused by Sporothrix brasiliensis is an emerging mycosis in Latin America. One of the problems to quickly treat infected animals and break the transmission chain is associated with the time-consuming gold-standard diagnosis method (culture). We aimed to evaluate a species-specific polymerase chain reaction (PCR) for the diagnosis of sporotrichosis caused by S. brasiliensis using non-invasive samples. We performed a retrospective cross-sectional study using samples collected with swabs from humans and cats with clinical suspicion of sporotrichosis. Deoxyribonucleic acid (DNA) was extracted using a commercial kit, and a species-specific PCR for S. brasiliensis detection was performed. One hundred ten samples were included. PCR showed a good concordance with culture (86% of agreement) for human and cat samples (Kappa coefficient\u00a0=\u00a00.722, and 0.727, respectively). In conclusion, our data shows that this adapted PCR using non-invasive samples can be applied to sporotrichosis diagnosis, being a good alternative mainly in regions with a lack of mycologists to identify the fungus in culture, contributing to the control of this emergent zoonosis.<\/jats:p>","DOI":"10.1093\/mmy\/myae075","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:08:16Z","timestamp":1721866096000},"source":"Crossref","is-referenced-by-count":3,"title":["<i>Sporothrix brasiliensis<\/i>-specific polymerase chain reaction for the diagnosis of cat and human sporotrichosis through non-invasive samples"],"prefix":"10.1093","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1407-8135","authenticated-orcid":false,"given":"Mariana Rodrigues","family":"Tr\u00e1paga","sequence":"first","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande (FURG) , Rio Grande, 96203-900 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7121-3861","authenticated-orcid":false,"given":"Vanice Rodrigues","family":"Poester","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande (FURG) , Rio Grande, 96203-900 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Ortiz","family":"Sanchotene","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Hospital Universidade Dr. Miguel Riet Corr\u00eaa Jr. (HU-FURG\/Ebserh) , Rio Grande, 96200-190 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana Patr\u00edcia","family":"Basso","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Hospital Universidade Dr. Miguel Riet Corr\u00eaa Jr. (HU-FURG\/Ebserh) , Rio Grande, 96200-190 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana Fedatto","family":"Bernardon","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Hospital Universidade Dr. Miguel Riet Corr\u00eaa Jr. (HU-FURG\/Ebserh) , Rio Grande, 96200-190 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rejane","family":"Luvielmo","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Hospital Universidade Dr. Miguel Riet Corr\u00eaa Jr. (HU-FURG\/Ebserh) , Rio Grande, 96200-190 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia Bittencourt","family":"Severo","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Basicas da Sa\u00fade, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA) , Porto Alegre, 3303-8804, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6639-6058","authenticated-orcid":false,"given":"Rodrigo","family":"Almeida-Paes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas , Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, 21040-900 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosely Maria","family":"Zancop\u00e9\u00a0Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas , Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, 21040-900 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"von\u00a0Groll","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande (FURG) , Rio Grande, 96203-900 , Brasil"},{"name":"N\u00facleo de Pesquisa em Microbiologia M\u00e9dica (NUPEMM) , FAMED-FURG, Rio Grande, 96203-900 , Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3883-0080","authenticated-orcid":false,"given":"Melissa Orzechowski","family":"Xavier","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Micologia M\u00e9dica, FAMED-FURG , Rio Grande, 96203-900, RS , Brasil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande (FURG) , Rio Grande, 96203-900 , 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