{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:28:22Z","timestamp":1789637302990,"version":"4.0.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,29]],"date-time":"2024-12-29T00:00:00Z","timestamp":1735430400000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bourse de recherche PAM Biologie-ACP des Hospices Civils de Lyon 2022"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cryptococcus neoformans\/gattii and Histoplasma capsulatum var. capsulatum may present atypical histopathological features inducing diagnostic errors. We aimed to estimate the frequency of these atypical features in formalin-fixed tissue (FT) samples and to assess the relevance of an integrated histomolecular diagnosis using specific H. capsulatum PCR and panfungal PCR followed by Sanger sequencing and\/or targeted massive parallel sequencing (MPS). A total of 27 FT from 23 patients with a histopathological diagnosis of cryptococcosis (n\u00a0=\u00a016 FT from 13 patients) or histoplasmosis (n\u00a0=\u00a011 FT from 10 patients) were retrospectively included. All FT were consultation cases. Mycological identifications on equivalent fresh tissue were available for 11\/23 (47.8%) patients. The expert pathologist review modified the diagnosis suggested by the initial pathologist in 7\/27 (25.9%) FT. Fungal morphology and tissue inflammation were compared between both mycoses. The most discriminant atypical criterion was the presence of dented-looking yeasts, observed in 68.75% (11\/16) of C. neoformans\/gattii and none (0\/11) of H. capsulatum var. capsulatum (P\u00a0=\u00a0.002). For the 12\/23 (52.2%) patients without mycological identification on fresh tissue, an integrated histomolecular diagnosis on FT using specific PCR or panfungal PCR followed by Sanger sequencing and\/or MPS led to fungal identification in 9\/12 (75%) cases; for cryptococcosis, the targeted MPS sensitivity was higher than that of Sanger sequencing (P\u00a0=\u00a0.041). Thus, because atypical histopathological features may be tricky, integrated histomolecular diagnosis is essential for optimal patient care.<\/jats:p>","DOI":"10.1093\/mmy\/myae126","type":"journal-article","created":{"date-parts":[[2024,12,29]],"date-time":"2024-12-29T03:23:32Z","timestamp":1735442612000},"source":"Crossref","is-referenced-by-count":10,"title":["<i>Cryptococcus neoformans\/gattii<\/i> and <i>Histoplasma capsulatum<\/i> var. <i>capsulatum<\/i> infections on tissue sections: Diagnostic pitfalls and relevance of an integrated histomolecular diagnosis"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1674-1888","authenticated-orcid":false,"given":"Alexis","family":"Trecourt","sequence":"first","affiliation":[{"name":"Service de Pathologie Multi-Site - Site Sud, Centre Hospitalier Lyon Sud, Hospices Civil de Lyons , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon-Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon 1, UR 3738 \u2013 CICLY \u2013 Equipe Inflammation et immunit\u00e9 de l\u2032\u00e9pith\u00e9lium respiratoire , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meja","family":"Rabodonirina","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Donzel","sequence":"additional","affiliation":[{"name":"Service de Pathologie Multi-Site - Site Sud, Centre Hospitalier Lyon Sud, Hospices Civil de Lyons , Lyon ,","place":["France"]},{"name":"Centre de Recherche en Canc\u00e9rologie de Lyon (CRCL), INSERM U1052, CNRS UMR 5286, Facult\u00e9 de M\u00e9decine Lyon Sud, Universit\u00e9 Claude Bernard Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Chapey-Picq","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrazzak","family":"Bentaher","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Lyon-Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon 1, UR 3738 \u2013 CICLY \u2013 Equipe Inflammation et immunit\u00e9 de l\u2032\u00e9pith\u00e9lium respiratoire , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6992-1073","authenticated-orcid":false,"given":"Damien","family":"Dupont","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charline","family":"Miossec","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Persat","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Lyon-Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon 1, UR 3738 \u2013 CICLY \u2013 Equipe Inflammation et immunit\u00e9 de l\u2032\u00e9pith\u00e9lium respiratoire , Lyon ,","place":["France"]},{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8285-8550","authenticated-orcid":false,"given":"Martine","family":"Wallon","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Lemoine","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-8174","authenticated-orcid":false,"given":"Pauline","family":"Tirard-Collet","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Lyon-Sud Charles M\u00e9rieux, Universit\u00e9 Claude Bernard Lyon 1, UR 3738 \u2013 CICLY \u2013 Equipe Inflammation et immunit\u00e9 de l\u2032\u00e9pith\u00e9lium respiratoire , Lyon ,","place":["France"]},{"name":"Service de Parasitologie et Mycologie M\u00e9dicale, Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Claude Bernard Lyon-1 , Lyon ,","place":["France"]},{"name":"G\u00e9nomique \u00e9pid\u00e9miologique des maladies infectieuses (GENEPII), Institut des Agents Infectieux, H\u00f4pital Croix-Rousse, Hospices Civils de Lyon , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Baltr\u00e8s","sequence":"additional","affiliation":[{"name":"Service de Pathologie, Centre L\u00e9on Berard , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-3082","authenticated-orcid":false,"given":"Alexandre","family":"Alanio","sequence":"additional","affiliation":[{"name":"Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, Assistance Publique-H\u00f4pitaux de Paris , Paris ,","place":["France"]},{"name":"Molecular Mycology Unit, CNRS UMR2000, Institut Pasteur , Paris 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