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Chitosan is a nontoxic, biodegradable, and biocompatible linear polysaccharide made from the deacetylation of chitin. In this study, a ZCF31 putative transcription factor gene was selected from a previous mutant library screen, as zcf31\u0394 strains exhibited defective cell growth in response to chitosan. Furthermore, chitosan caused notable damage to zcf31\u0394 cells; however, ZCF31 expression was not significantly changed by chitosan, suggesting that zcf31\u0394 is sensitive to chitosan could be due to changes in the physical properties of C. albicans. Indeed, zcf31\u0394 cells displayed significant increases in cell wall thickness. Consistent with the previous study, zcf31\u0394 strains were resistant to calcofluor white but highly susceptible to SDS (sodium dodecyl sulfate). These results implied that chitosan mainly influences membrane function, as zcf31\u0394 strengthens the stress resistance of the fungal cell wall but lessens cell membrane function. Interestingly, this effect on the cell surface mechanics of the C. albicans zcf31\u0394 strains was not responsible for the virulence-associated function. RNA-seq analysis further revealed that six mannosyltransferase-related genes were upregulated in zcf31\u0394. Although five mannosyltransferase-related mutant strains in the zcf31\u0394 background partially reduced the cell wall thickness, only zcf31\u0394\/mnn45\u0394 showed the recovery of chitosan resistance. Our findings suggest that Zcf31 mediates a delicate and complicated dynamic balance between the cell membrane and cell wall architectures through the mannosyltransferase genes in C. albicans, leading to altered chitosan susceptibility.<\/jats:p>","DOI":"10.1093\/mmy\/myaf025","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T09:57:56Z","timestamp":1742723876000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>MNN45<\/i> is involved in Zcf31-mediated cell surface integrity and chitosan susceptibility in <i>Candida albicans<\/i>"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5041-9705","authenticated-orcid":false,"given":"Hao-Sen","family":"Chiang","sequence":"first","affiliation":[{"name":"Department of Life Science, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]},{"name":"Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chun","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]},{"name":"Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chieh","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai-Ling","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Ting","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chiao","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao-Yen","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8894-6196","authenticated-orcid":false,"given":"Ching-Hsuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei , , 10617","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"issue":"2","key":"2025040814543921900_bib1","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1179\/joc.2004.16.2.172","article-title":"Hospital-acquired candidemia in HIV-infected patients. 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