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This study assessed the role of phenotypic switching in hyperosmotic stress adaptation. Two C. tropicalis strains (49.07 and 100.10), each with five morphotypes, were exposed to 1\u00a0M NaCl for 10 and 60 min. Results showed that 62.5% of morphotypes were less sensitive to osmotic shock, and 50% had greater osmoadaptation. The crepe morphotype exhibited less cell damage. Changes in cell wall composition, porosity, and expression of EFG1, WOR1, and HOG1 were observed. 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