{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:18Z","timestamp":1788568578542,"version":"build-2803163510"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Aspergillus polymerase chain reaction (PCR) now employs standardised methodology and commercial options, with clinical performance validated through meta-analyses. The long-term availability of external quality assessment schemes drives performance and provides a currently untapped source of technical data. The Fungal PCR initiative and the Quality Control for Molecular Diagnostics (QCMD) combined forces to evaluate data from the Aspergillus PCR QCMD schemes between 2019 and 2022. Analysis showed inferior detection of non-fumigatus Aspergillus species (i.e.,Aspergillus niger) compared with A. fumigatus. Longitudinal evaluation showed the use of \u2018in-house\u2019 PCR methods by new participants when testing plasma generated lower positivity rates compared to commercial assays.<\/jats:p>","DOI":"10.1093\/mmy\/myaf036","type":"journal-article","created":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:30:12Z","timestamp":1744417812000},"source":"Crossref","is-referenced-by-count":1,"title":["A longitudinal study assessing <i>Aspergillus<\/i> polymerase chain reaction (PCR) performance through participation in external quality assessment schemes from 2019 to 2022"],"prefix":"10.1093","volume":"63","author":[{"given":"Alastair","family":"Ricketts","sequence":"first","affiliation":[{"name":"QCMD , Glasgow, Scotland, G20 0XA ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Montgomery","sequence":"additional","affiliation":[{"name":"QCMD , Glasgow, Scotland, G20 0XA ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Loeffler","sequence":"additional","affiliation":[{"name":"Medizinische Klinik II, Labor W\u00dc4i, Universit\u00e4tsklinikum W\u00fcrzburg , 97080 W\u00fcrzburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Barnes","sequence":"additional","affiliation":[{"name":"Cardiff University Department of Infection and Immunity, , Cardiff, CF14 4XN ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1641-7367","authenticated-orcid":false,"given":"Mario","family":"Cruciani","sequence":"additional","affiliation":[{"name":"The Fungal PCR Initiative , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Peter","family":"Donnelly","sequence":"additional","affiliation":[{"name":"The European Aspergillus PCR Initiative, de Hoefkamp 1096 , 6545 MD, Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3056-4205","authenticated-orcid":false,"given":"P Lewis","family":"White","sequence":"additional","affiliation":[{"name":"Public Health Wales Mycology Reference Laboratory and Cardiff University Centre for trials research, University Hospital of Wales, Heath Park , Cardiff, CF14 4XW ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"issue":"6","key":"2025043020054814300_bib1","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and update of the consensus definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin Infect Dis"},{"issue":"158","key":"2025043020054814300_bib2","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1080\/01621459.1927.10502953","article-title":"Probable inference, the law of succession, and statistical inference","volume":"22","author":"Wilson","year":"1927","journal-title":"J Am Statist Assoc"},{"issue":"8","key":"2025043020054814300_bib3","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1002\/(sici)1097-0258(19980430)17:8&lt;873:aid-sim779&gt;3.0.co;2-i","article-title":"Interval estimation for the difference between independent proportions: Comparison of eleven methods","volume":"17","author":"Newcombe","year":"1998","journal-title":"Stat Med"},{"issue":"4","key":"2025043020054814300_bib4","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1093\/mmy\/myw093","article-title":"Determining the analytical specificity of PCR-based assays for the diagnosis of IA: What is Aspergillus?","volume":"55","author":"Morton","year":"2017","journal-title":"Med Mycol"},{"key":"2025043020054814300_bib5","doi-asserted-by":"publisher","first-page":"683","DOI":"10.3389\/fmicb.2016.00683","article-title":"Aspergillus fumigatus-related species in clinical practice","volume":"7","author":"Lamoth","year":"2016","journal-title":"Front Microbiol"},{"issue":"11","key":"2025043020054814300_bib6","doi-asserted-by":"publisher","first-page":"3842","DOI":"10.1128\/JCM.05316-11","article-title":"Evaluation of Aspergillus PCR protocols for testing serum specimens","volume":"49","author":"White","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"4","key":"2025043020054814300_bib7","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1128\/JCM.01767-09","article-title":"Aspergillus PCR: One step closer to standardization","volume":"48","author":"White","year":"2010","journal-title":"J Clin Microbiol"}],"container-title":["Medical Mycology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/mmy\/advance-article-pdf\/doi\/10.1093\/mmy\/myaf036\/62916591\/myaf036.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mmy\/article-pdf\/63\/5\/myaf036\/62916591\/myaf036.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mmy\/article-pdf\/63\/5\/myaf036\/62916591\/myaf036.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:05:54Z","timestamp":1746057954000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mmy\/article\/doi\/10.1093\/mmy\/myaf036\/8111604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":7,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,4,30]]}},"URL":"https:\/\/doi.org\/10.1093\/mmy\/myaf036","relation":{},"ISSN":["1369-3786","1460-2709"],"issn-type":[{"value":"1369-3786","type":"print"},{"value":"1460-2709","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,5]]},"published":{"date-parts":[[2025,4,11]]},"article-number":"myaf036"}}