{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:45:30Z","timestamp":1777574730334,"version":"3.51.4"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health Agency for Health Research","award":["NU23-05-00095"],"award-info":[{"award-number":["NU23-05-00095"]}]},{"DOI":"10.13039\/501100006704","name":"University of Ostrava","doi-asserted-by":"publisher","award":["SGS08\/LF\/2023"],"award-info":[{"award-number":["SGS08\/LF\/2023"]}],"id":[{"id":"10.13039\/501100006704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports","doi-asserted-by":"publisher","award":["CZ.02.01.01\/00\/22_008\/0004597"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004597"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pentraxin 3 (Ptx3) is an acute-phase protein that specifically targets fungal galactosaminogalactan and has been proposed as a promising biomarker for invasive fungal infections. However, its stability in clinical samples over time has not yet been established. This study aimed to evaluate the stability of Ptx3 in serum and bronchoalveolar lavage fluid (BALF) samples during mid- and long-term storage. A total of 44 serum and 52 BALF samples were examined or re-examined for Ptx3 concentrations using enzyme immunoassay in pooled and individual sample formats. Samples were stored at \u221280\u00b0C, \u221220\u00b0C, and\u00a0+37\u00b0C for periods ranging from 0 to 56 months. Statistical analyses included a paired two-sample Wilcoxon signed-rank test, analysis of variance, Bonferroni test, and linear regression analysis. Ptx3 remained highly stable in serum and BALF samples for up to 8 months at \u221220\u00b0C, with variations ranging from \u22121.8% to\u00a0+2.8%. Long-term stability was observed at \u221280\u00b0C for 48 months, followed by a slow decline in Ptx3 levels. In contrast, storage at\u00a0+37\u00b0C resulted in rapid degradation, with a 36.5%\u201360.7% increase or a 92.9%\u201397% decrease in Ptx3 levels in serum and BALF, respectively. These findings confirm that Ptx3 is a stable and reliable biomarker for invasive fungal infections when appropriate storage conditions are maintained.<\/jats:p>","DOI":"10.1093\/mmy\/myaf057","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T10:35:35Z","timestamp":1751020535000},"source":"Crossref","is-referenced-by-count":1,"title":["Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4299-9169","authenticated-orcid":false,"given":"Radim","family":"Dobi\u00e1\u0161","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava Department of Bacteriology and Mycology, , Ostrava ,","place":["Czech Republic"]},{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7466-7535","authenticated-orcid":false,"given":"Valeria","family":"Skopelidou","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]},{"name":"Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava , Ostrava ,","place":["Czech Republic"]},{"name":"Institute of Molecular and Clinical Pathology and Medical Genetics, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea Langer","family":"Serme\u00f1o","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Strako\u0161","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominika","family":"Lupt\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences , Prague ,","place":["Czech Republic"]},{"name":"Biomedicine Research Centre of the Slovak Academy of Sciences, Institute of Virology , Bratislava ,","place":["Slovakia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Tom\u00e1\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava Department of Bacteriology and Mycology, , Ostrava ,","place":["Czech Republic"]},{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Ra\u0161ka","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Dentistry, Palack\u00fd University and University Hospital Department of Immunology, , Olomouc ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3579-3066","authenticated-orcid":false,"given":"Jozef","family":"\u0160karda","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava , Ostrava ,","place":["Czech Republic"]},{"name":"Institute of Molecular and Clinical Pathology and Medical Genetics, Faculty of Medicine, University of Ostrava , Ostrava ,","place":["Czech 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