{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T14:25:55Z","timestamp":1766586355897,"version":"3.48.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Invasive aspergillosis (IA), caused by Aspergillus, requires prompt diagnosis and treatment. Here, the efficacy of a chemiluminescence immunoassay (CLIA) for detecting Aspergillus galactomannan (GM) antigen in serum and bronchoalveolar lavage fluid (BALF) was evaluated. Overall, 265 patients with suspected IA were enrolled between March and May 2023 and were stratified into IA (n\u00a0=\u00a048) and non-IA (n\u00a0=\u00a0217) cohorts. A total of 265 samples (208 serum, 57 BALF) were analyzed with GM-CLIA. The specificity, sensitivity, negative predictive value (NPV), and positive predictive value (PPV) were calculated. Diagnostic performance was assessed with receiver operating characteristic (ROC) curves. GM-CLIA demonstrated sensitivity\/specificity values of 88.00%\/93.44% in serum and 91.30%\/76.47% in BALF. The sensitivity for all samples was 89.58% with a specificity of 90.78%, while PPV and NPV were 69.25% and 97.52%, respectively. The AUC (area under the ROC curve) was 0.93 (95% confidence interval [CI], 0.89\u20130.97, P\u00a0&amp;lt;\u00a0.001), with an optimal cutoff value of 0.70\u00a0ng\/ml. The GM-CLIA enables automated detection of individual samples (serum\/BALF) on a fully integrated chemiluminescence platform. This system delivers high specificity (90.78%), sensitivity (89.58%), enabling prompt diagnosis and treatment of IA.<\/jats:p>","DOI":"10.1093\/mmy\/myaf111","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T12:56:44Z","timestamp":1764680204000},"source":"Crossref","is-referenced-by-count":0,"title":["Rapid and high-throughput chemiluminescence immunoassay for detection of\n                    <i>Aspergillus<\/i>\n                    galactomannan"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5895-6799","authenticated-orcid":false,"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"Qilu Hospital of Shandong University Department of Clinical Laboratory, , Jinan 250012 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuyao","family":"Hu","sequence":"additional","affiliation":[{"name":"Qilu Hospital of Shandong University Department of Clinical Laboratory, , Jinan 250012 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Pan","sequence":"additional","affiliation":[{"name":"Qilu Hospital of Shandong University Department of Clinical Laboratory, , Jinan 250012 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Qilu Hospital of Shandong University Department of Clinical Laboratory, , Jinan 250012 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Yao","sequence":"additional","affiliation":[{"name":"Tianjin Enterprise Key Laboratory for Precision Diagnosis Technology of Invasive Fungal Diseases , Tianjin 300000 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Enterprise Key Laboratory for Precision Diagnosis Technology of Invasive Fungal Diseases , Tianjin 300000 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Enterprise Key Laboratory for Precision Diagnosis Technology of Invasive Fungal Diseases , Tianjin 300000 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Qilu Hospital of Shandong University Department of Clinical Laboratory, , Jinan 250012 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"issue":"5","key":"2025122409221401600_bib1","doi-asserted-by":"publisher","first-page":"e268","DOI":"10.1016\/S1473-3099(24)00102-6","article-title":"Global incidence and mortality of severe fungal disease","volume":"24","author":"Ikuta","year":"2024","journal-title":"Lancet Infect Dis"},{"key":"2025122409221401600_bib2","doi-asserted-by":"publisher","first-page":"1587227","DOI":"10.3389\/fmicb.2025.1587227","article-title":"Evaluation of the clinical characteristics and survival outcomes of invasive pulmonary aspergillosis patients","volume":"16","author":"Feng","year":"2025","journal-title":"Front Microbiol"},{"issue":"2","key":"2025122409221401600_bib3","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.diagmicrobio.2018.01.014","article-title":"Invasive pulmonary aspergillosis and influenza co-infection in immunocompetent hosts: case reports and review of the literature","volume":"91","author":"Shah","year":"2018","journal-title":"Diagn Microbiol Infect Dis."},{"issue":"4","key":"2025122409221401600_bib4","doi-asserted-by":"publisher","first-page":"557","DOI":"10.4103\/ijmm.IJMM_18_366","article-title":"Evaluation of biomarkers: Galactomannan and 1,3-beta-D-glucan assay for the diagnosis of invasive fungal infections in immunocompromised patients from a tertiary care centre","volume":"36","author":"Singh","year":"2018","journal-title":"Indian J Med Microbiol"},{"issue":"5","key":"2025122409221401600_bib5","doi-asserted-by":"publisher","first-page":"e37067","DOI":"10.1097\/MD.0000000000037067","article-title":"Diagnostic performance of serum galactomannan and \u03b2-D-glucan for invasive aspergillosis in suspected patients: A meta-analysis","volume":"103","author":"Huang","year":"2024","journal-title":"Medicine (Baltimore)"},{"issue":"7","key":"2025122409221401600_bib6","doi-asserted-by":"publisher","first-page":"2153","DOI":"10.1128\/JCM.00345-17","article-title":"Diagnostic value of galactomannan antigen test in serum and bronchoalveolar lavage fluid samples from patients with nonneutropenic invasive pulmonary aspergillosis. 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