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We assessed the \u2018marble method\u2019 to evenly spread CSF onto two Sabouraud dextrose agar plates compared with the \u2018droplet method\u2019 using drops of CSF evenly placed around two halves of one agar plate. We found high concordance between the two methods. The Bland\u2013Altman plot showed that the droplet method differed from the marble method by an average of \u22120.044 log10 CFU\/ml CSF, with 95% limits of agreement from \u20130.324 to 0.235, indicating a small bias in favor of the marble method (P\u00a0&amp;lt;\u00a00.001). The droplet method of quantitative cryptococcal culture had no clinical difference compared to the marble method.<\/jats:p>","DOI":"10.1093\/mmy\/myaf120","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T13:04:33Z","timestamp":1765976673000},"source":"Crossref","is-referenced-by-count":0,"title":["Analytical validation: Comparison of two laboratory-based methods for CSF quantitative cryptococcal cultures"],"prefix":"10.1093","volume":"64","author":[{"given":"Andrew","family":"Akampurira","sequence":"first","affiliation":[{"name":"College of Health Science, Makerere University Department of Microbiology, , Kampala ,","place":["Uganda"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7226-3075","authenticated-orcid":false,"given":"Thomas C","family":"McHale","sequence":"additional","affiliation":[{"name":"University of Minnesota Department of Medicine, , Minneapolis, MN 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