{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:29:18Z","timestamp":1787059758346,"version":"build-2736575974"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Canisius-Wilhelmina Hospital","award":["CWZ_001421"],"award-info":[{"award-number":["CWZ_001421"]}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["DDR\/IIRP23\/3633"],"award-info":[{"award-number":["DDR\/IIRP23\/3633"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pichia kudriavzevii (previously known as Candida krusei) is among the top five causative agents of candidemia, mainly affecting neonates. The yeast is intrinsically resistant to fluconazole, and nosocomial transmission has been reported. Recently, whole genome sequencing (WGS) analysis on 24 out of 165 clinical P. kudriavzevii isolates from Delhi, India showed two clusters, indicating nosocomial transmission. To further increase our insight into the transmission of this fungus in healthcare settings, we performed short tandem repeat (STR) genotyping of 106 isolates from Delhi, India, and compared outcomes to STR genotypes and WGS data of isolates originating from various countries. A total of 52 unique genotypes were identified, in addition to five clusters comprising two to 22 isolates. Four of these clusters were restricted to a single hospital, while the largest cluster included six hospitals. Interestingly, when compared to a global collection, Iranian clinical isolates were identical to the multicenter cluster and also related to isolates from the Netherlands and Sri Lanka, the latter isolate being collected in 1935. WGS analysis on a selection of isolates confirmed the clusters identified by STR analysis, underscoring the high discriminatory power of the STR genotyping scheme. In short, we report several events of single-center nosocomial transmission of P. kudriavzevii in India. One multicenter cluster was found, which was closely related to isolates originating from diverse countries, suggesting a fitness advantage or elevated transmission potential for this lineage.<\/jats:p>","DOI":"10.1093\/mmy\/myag082","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:51:50Z","timestamp":1785930710000},"source":"Crossref","is-referenced-by-count":0,"title":["Short tandem repeat genotyping of\n                    <i>Pichia kudriavzevii<\/i>\n                    isolates reveals long-standing clinical clade with high transmission potential"],"prefix":"10.1093","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6734-361X","authenticated-orcid":false,"given":"Bram","family":"Spruijtenburg","sequence":"first","affiliation":[{"name":"Radboudumc, 6525 GA Department of Medical Microbiology, , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ , Nijmegen ,","place":["The Netherlands"]},{"name":"Canisius-Wilhelmina Hospital (CWZ)\/Dicoon, 6532 SZ Department of Medical Microbiology and Immunology, , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8417-5784","authenticated-orcid":false,"given":"Kusum","family":"Jain","sequence":"additional","affiliation":[{"name":"Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, 110007 , Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merlijn H I","family":"van\u00a0Haren","sequence":"additional","affiliation":[{"name":"Radboudumc, 6525 GA Department of Medical Microbiology, , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-6080","authenticated-orcid":false,"given":"Jacques F","family":"Meis","sequence":"additional","affiliation":[{"name":"Radboudumc, 6525 GA Department of Medical Microbiology, , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ , Nijmegen ,","place":["The Netherlands"]},{"name":"Institute of Translational Research, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Excellence Center for Medical Mycology, University of Cologne, 50923 , Cologne ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theun","family":"de\u00a0Groot","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ , Nijmegen ,","place":["The Netherlands"]},{"name":"Canisius-Wilhelmina Hospital (CWZ)\/Dicoon, 6532 SZ Department of Medical Microbiology and Immunology, , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0226-024X","authenticated-orcid":false,"given":"Eelco F J","family":"Meijer","sequence":"additional","affiliation":[{"name":"Radboudumc, 6525 GA Department of Medical Microbiology, , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ , Nijmegen ,","place":["The Netherlands"]},{"name":"Canisius-Wilhelmina Hospital (CWZ)\/Dicoon, 6532 SZ Department of Medical Microbiology and Immunology, , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, 110007 , Delhi ,","place":["India"]},{"name":"National Reference Laboratory for Antimicrobial Resistance in Fungal Pathogens, Vallabhbhai Patel Chest Institute, University of Delhi, 110007 , Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"issue":"7","key":"2026081809065547000_bib1","doi-asserted-by":"publisher","first-page":"e428","DOI":"10.1016\/S1473-3099(23)00692-8","article-title":"Global incidence and mortality of severe fungal disease","volume":"24","author":"Denning","year":"2025","journal-title":"Lancet Infect Dis"},{"issue":"5","key":"2026081809065547000_bib2","doi-asserted-by":"publisher","first-page":"e280","DOI":"10.1016\/S1473-3099(24)00749-7","article-title":"Global guideline for the diagnosis and management of candidiasis: an initiative of the ECMM in cooperation with ISHAM and ASM","volume":"25","author":"Cornely","year":"2025","journal-title":"Lancet Infect 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Mycol."},{"issue":"1","key":"2026081809065547000_bib6","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1053\/jhin.2001.1131","article-title":"Increase in prevalence of nosocomial non-Candida albicans candidaemia and the association of Candida krusei with fluconazole use","volume":"50","author":"Hope","year":"2002","journal-title":"J Hosp Infect."},{"issue":"1","key":"2026081809065547000_bib7","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1093\/mmy\/myaa031","article-title":"Update on Candida krusei, a potential multidrug-resistant pathogen","volume":"59","author":"Jamiu","year":"2021","journal-title":"Med Mycol."},{"issue":"7","key":"2026081809065547000_bib8","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1007\/s12098-025-05593-9","article-title":"Neonatal invasive candidiasis: current concepts","volume":"92","author":"Ferreras-Antolin","year":"2025","journal-title":"Indian J 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