{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:16Z","timestamp":1790718676415,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,4,1]]},"DOI":"10.1093\/mmy\/myy020","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T15:10:05Z","timestamp":1523373005000},"page":"277-283","source":"Crossref","is-referenced-by-count":58,"title":["Clinical evaluation of the DermaGenius\u00ae Nail real-time PCR assay for the detection of dermatophytes and <i>Candida albicans<\/i> in nails"],"prefix":"10.1093","volume":"57","author":[{"given":"Marie-Pierre","family":"Hayette","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"},{"name":"National Reference Center for Mycosis, University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Seidel","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Adjetey","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajae","family":"Darfouf","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"},{"name":"National Reference Center for Mycosis, University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"W\u00e9ry","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00ebl","family":"Boreux","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalie","family":"Sacheli","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"},{"name":"National Reference Center for Mycosis, University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierrette","family":"Melin","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Arrese","sequence":"additional","affiliation":[{"name":"Department of Dermatopathology, University Hospital of Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,5,11]]},"reference":[{"key":"key\n\t\t\t\t2019030416255050200_bib1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1439-0507.2008.01606.x","article-title":"Epidemiological trends in skin mycoses worldwide","volume":"51","author":"Havlickova","year":"2008","journal-title":"Mycoses"},{"key":"key\n\t\t\t\t2019030416255050200_bib2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.clindermatol.2009.12.005","article-title":"Epidemiology of superficial fungal infections","volume":"28","author":"Ameen","year":"2010","journal-title":"Clin Dermatol"},{"key":"key\n\t\t\t\t2019030416255050200_bib3","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s11046-008-9100-9","article-title":"Updates on the epidemiology of dermatophyte infections","volume":"166","author":"Seebacher","year":"2008","journal-title":"Mycopathologia"},{"key":"key\n\t\t\t\t2019030416255050200_bib4","first-page":"188","article-title":"Mycology - an update. Part 1: Dermatomycoses: causative agents, epidemiology and pathogenesis","volume":"12","author":"Nenoff","year":"2014","journal-title":"J Dtsch Dermatol Ges"},{"key":"key\n\t\t\t\t2019030416255050200_bib5","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s12281-015-0231-4","article-title":"Dermatophytosis, trends in epidemiology and diagnostic approach","volume":"9","author":"Hayette","year":"2015","journal-title":"Curr Fungal Infect Rep"},{"key":"key\n\t\t\t\t2019030416255050200_bib6","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1111\/jdv.12323","article-title":"The prevalence of onychomycosis in the global population? A literature study","volume":"28","author":"Sigurgeirsson","year":"2014","journal-title":"J Eur Acad Dermatology Venereol"},{"key":"key\n\t\t\t\t2019030416255050200_bib7","first-page":"749","article-title":"Mycology, an update. Part 2: Dermatomycoses: clinical picture and diagnostics","volume":"12","author":"Nenoff","year":"2014","journal-title":"J Dtsch Dermatol Ges"},{"key":"key\n\t\t\t\t2019030416255050200_bib8","first-page":"29","article-title":"Dermatophytes: the mycological diagnostic challenge","volume":"2008","author":"Chabasse","year":"2008","journal-title":"Rev Francoph des Lab"},{"key":"key\n\t\t\t\t2019030416255050200_bib9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1469-0691.2005.01321.x","article-title":"Comparison of two fluorescent whiteners, calcofluor and blankophor, for the detection of fungal elements in clinical specimens in the diagnostic laboratory","volume":"12","author":"Hamer","year":"2006","journal-title":"Clin Microbiol Infect"},{"key":"key\n\t\t\t\t2019030416255050200_bib10","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1111\/myc.12491","article-title":"Dermatophytosis: Fluorostaining enhances speed and sensitivity in direct microscopy of skin, nail, and hair specimens from dermatology outpatients","volume":"59","author":"Ovr\u00e9n","year":"2016","journal-title":"Mycoses"},{"key":"key\n\t\t\t\t2019030416255050200_bib11","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/j.1469-0691.2009.02991.x","article-title":"Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material","volume":"16","author":"Bergmans","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"key\n\t\t\t\t2019030416255050200_bib12","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/myc.12471","article-title":"Optimising the diagnostic strategy for onychomycosis from sample collection to FUNGAL identification evaluation of a diagnostic kit for real-time PCR","volume":"59","author":"Petinataud","year":"2016","journal-title":"Mycoses"},{"key":"key\n\t\t\t\t2019030416255050200_bib13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/myc.12097","article-title":"Clinical evaluation of a novel commercial multiplex-based PCR diagnostic test for differential diagnosis of dermatomycoses","volume":"57","author":"Mehlig","year":"2014","journal-title":"Mycoses"},{"key":"key\n\t\t\t\t2019030416255050200_bib14","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1128\/JCM.39.2.685-690.2001","article-title":"Rapid discrimination among dermatophytes, Scytalidium spp., and other fungi with a PCR\u2013restriction fragment length polymorphism ribotyping method","volume":"39","author":"Machouart-Dubach","year":"2001","journal-title":"J Clin Microbiol"},{"key":"key\n\t\t\t\t2019030416255050200_bib15","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/j.1365-2133.2004.06089.x","article-title":"Polymerase chain reaction for diagnosis of dermatophyte and Scytalidium spp. onychomycosis","volume":"151","author":"Menotti","year":"2004","journal-title":"Br J Dermatol"},{"key":"key\n\t\t\t\t2019030416255050200_bib16","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1128\/JCM.01418-06","article-title":"Multicenter evaluation of a commercial PCR-enzyme-linked immunosorbent assay diagnostic kit (onychodiag) for diagnosis of dermatophytic onychomycosis","volume":"45","author":"Savin","year":"2007","journal-title":"J Clin Microbiol"},{"key":"key\n\t\t\t\t2019030416255050200_bib17","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.mimet.2011.01.023","article-title":"Trapped in keratin: a comparison of dermatophyte detection in nail, skin, and hair samples directly from clinical samples using culture and real-time PCR","volume":"85","author":"Wisselink","year":"2011","journal-title":"J Microbiol Methods"},{"key":"key\n\t\t\t\t2019030416255050200_bib18","doi-asserted-by":"crossref","first-page":"735.e11","DOI":"10.1016\/j.cmi.2016.05.015","article-title":"The agony of choice in dermatophyte diagnostics? Performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton interdigitale","volume":"22","author":"Kupsch","year":"2016","journal-title":"Clin Microbiol Infect"},{"key":"key\n\t\t\t\t2019030416255050200_bib19","first-page":"721","article-title":"Diagnostic PCR of dermatophytes: an overview","volume":"10","author":"Gr\u00e4ser","year":"2012","journal-title":"J der Dtsch Dermatologischen Gesellschaft"},{"key":"key\n\t\t\t\t2019030416255050200_bib20","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s12879-017-2258-3","article-title":"Meta-analysis of the utility of culture, biopsy, and direct KOH examination for the diagnosis of onychomycosis","volume":"17","author":"Velasquez-Agudelo","year":"2017","journal-title":"BMC Infect Dis"},{"key":"key\n\t\t\t\t2019030416255050200_bib21","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1159\/000051640","article-title":"Onychomycosis and other superficial fungal infections of the foot in the elderly: a pan-European survey","volume":"202","author":"Pi\u00e9rard","year":"2001","journal-title":"Dermatology"},{"key":"key\n\t\t\t\t2019030416255050200_bib22","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1097\/00000372-199612000-00011","article-title":"Onychomycosis and keratomycosis caused by Alternaria sp.: a bipolar opportunistic infection in a wood-pulp worker on chronic steroid therapy","volume":"18","author":"Arrese","year":"1996","journal-title":"Am J Dermatopathol"},{"key":"key\n\t\t\t\t2019030416255050200_bib23","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1159\/000093862","article-title":"Spores, sporodochia and fomites in onychomycosis","volume":"213","author":"Pi\u00e9rard","year":"2006","journal-title":"Dermatology"},{"key":"key\n\t\t\t\t2019030416255050200_bib24","doi-asserted-by":"crossref","first-page":"285.e9","DOI":"10.1016\/j.cmi.2015.11.012","article-title":"A one-year survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterization","volume":"22","author":"Sacheli","year":"2016","journal-title":"Clin Microbiol Infect"},{"key":"key\n\t\t\t\t2019030416255050200_bib25","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1440-0960.2007.00320.x","article-title":"Comparison of direct smear, culture, and histology for the diagnosis of onychomycosis","volume":"48","author":"Karimzadegan-Nia","year":"2007","journal-title":"Australas J Dermatol"},{"key":"key\n\t\t\t\t2019030416255050200_bib26","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1439-0507.2000.00602.x","article-title":"Case report and review: Onychomycosis due to Candida parapsilosis","volume":"43","author":"Gautret","year":"2000","journal-title":"Mycoses"},{"key":"key\n\t\t\t\t2019030416255050200_bib27","first-page":"1","article-title":"Diagnosis of dermatophytosis using molecular biology","volume":"182","author":"Verrier","year":"2016","journal-title":"Mycopathologia"}],"container-title":["Medical Mycology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/mmy\/article-pdf\/57\/3\/277\/28010277\/myy020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T16:26:09Z","timestamp":1551716769000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mmy\/article\/57\/3\/277\/4995299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,11]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,5,11]]},"published-print":{"date-parts":[[2019,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/mmy\/myy020","relation":{},"ISSN":["1369-3786","1460-2709"],"issn-type":[{"value":"1369-3786","type":"print"},{"value":"1460-2709","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,4]]},"published":{"date-parts":[[2018,5,11]]}}}