{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:24:04Z","timestamp":1781529844054,"version":"3.54.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"State-Region Planning Contracts"},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Poitiers"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Candida albicans can form biofilm on tissues and medical devices, becoming, in that case, less susceptible to antifungal agents. Treatment of candidiasis associated with the formation of C. albicans biofilms is restricted to echinocandins and lipid forms of amphotericin B. This study investigated the activity of micafungin and resveratrol modified molecule (EB487) against C. albicans biofilms. The anti-biofilm growth (Bgrowth) and anti-preformed biofilm (Bpreformed) activities of micafungin (0 to 3.94 \u03bcM) and EB487 (0 to 20.32\u00a0mM) were comparatively studied separately and combined, using XTT, flow cytometry and cell counts approaches. Concentrations causing 50% inhibition of the studied steps (IC50) were evaluated. When tested separately, IC50 Bgrowth was obtained for 4.8\u00a0mM and 0.13 \u03bcM of EB487 and micafungin respectively, and IC50 Bpreformed for 3.6\u00a0mM and 0.06 \u03bcM of EB487 and micafungin respectively. Micafungin used alone was not able to totally eradicate fungi. Micafungin combined with EB487 displayed synergistic activity (both anti-growth- and anti-preformed biofilm-activities). Optimal combination concentrations were EB487 (\u22649.12\u00a0mM -strain ATCC 28367\u2122 or \u22648.12\u00a0mM -strain CAI4-p), micafungin (\u22640.05 \u03bcM for both) and caused a total eradication of fungi. Dose reduction indexes obtained using these concentrations were at least 9 (micafungin) and 3.2 (EB487) for both anti-biofilm growth- and anti-preformed biofilm-activities. Combinations indexes were consistently below one, demonstrating a synergistic relationship between micafungin and EB487 in these conditions. This study demonstrated the strong anti-biofilm activity of EB487 and highlighted its synergistic potential when combined with micafungin. EB487 is a promising semi-synthetic molecule with prophylactic and curative interests in fighting C. albicans biofilms.<\/jats:p>","DOI":"10.1093\/mmy\/myz087","type":"journal-article","created":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T19:10:00Z","timestamp":1564773000000},"page":"530-542","source":"Crossref","is-referenced-by-count":7,"title":["Anti-biofilm activity of a semi-synthetic molecule obtained from resveratrol against Candida albicans biofilm"],"prefix":"10.1093","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0377-8312","authenticated-orcid":false,"given":"Camille","family":"Juin","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavie","family":"Perrin","sequence":"first","affiliation":[{"name":"University of Poitiers, Institute of Chemistry, Materials and Naturals Resources of Poitiers, IC2MP, UMR CNRS 7285, University of Poitiers, 4 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Puy","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment","family":"Bernard","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Laure","family":"Mollichella","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Girardot","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Costa","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Guillard","sequence":"first","affiliation":[{"name":"University of Poitiers, Institute of Chemistry, Materials and Naturals Resources of Poitiers, IC2MP, UMR CNRS 7285, University of Poitiers, 4 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-5000","authenticated-orcid":false,"given":"Christine","family":"Imbert","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Poitiers, Laboratoire Ecologie Biologie des Interactions, UMR CNRS 7267, 1 rue Michel Brunet TSA 51106 86073 Poitiers Cedex 9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,8,28]]},"reference":[{"key":"2020053120000836200_bib1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S0966-842X(02)00002-1","article-title":"Candida biofilms and their role in infection","volume":"11","author":"Douglas","year":"2003","journal-title":"Trends Microbiol"},{"key":"2020053120000836200_bib2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1111\/myc.12147","article-title":"Effects of ambroxol on Candida albicans growth and biofilm formation","volume":"57","author":"Rene","year":"2014","journal-title":"Mycoses"},{"key":"2020053120000836200_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/jof4010005","article-title":"Recent insights into the paradoxical effect of echinocandins","volume":"4","author":"Wagener","year":"2017","journal-title":"J Fungi"},{"key":"2020053120000836200_bib4","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1016\/j.bmcl.2018.04.024","article-title":"Biofilm inhibition and anti-Candida activity of a cationic lipo-benzamide molecule with twin-nonyl chain","volume":"28","author":"Jain","year":"2018","journal-title":"Bioorganic Med Chem Lett"},{"issue":"3","key":"2020053120000836200_bib5","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1128\/AAC.01397-07","article-title":"Synergistic effect of calcineurin inhibitors and fluconazole against Candida albicans biofilms","volume":"52","author":"Uppuluri","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"2020053120000836200_bib6","first-page":"1","article-title":"Review on combinatorial approach for inhibiting Candida albicans biofilm","volume":"1","author":"Mortale","year":"2018","journal-title":"Am J Clin Microbiol Antimicrob"},{"key":"2020053120000836200_bib7","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1128\/AAC.46.6.1773-1780.2002","article-title":"Antifungal susceptibility of Candida biofilms: unique efficacy of amphotericin B lipid formulations and echinocandins","volume":"46","author":"Kuhn","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"2020053120000836200_bib8","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1080\/13693780802428542","article-title":"Paradoxical echinocandin activity: a limited in vitro phenomenon","volume":"47","author":"Wiederhold","year":"2009","journal-title":"Med Mycol"},{"key":"2020053120000836200_bib9","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1128\/AAC.00999-10","article-title":"Paradoxical effect of caspofungin against Candida bloodstream isolates is mediated by multiple pathways but eliminated in human serum","volume":"55","author":"Shields","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"2020053120000836200_bib10","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/pathogens7010006","article-title":"What could be the role of antifungal lock-solutions? from bench to bedside","volume":"7","author":"Imbert","year":"2018","journal-title":"Pathogens"},{"key":"2020053120000836200_bib11","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s12223-017-0555-2","article-title":"In vitro activity of micafungin against biofilms of Candida albicans, Candida glabrata, and Candida parapsilosis at different stages of maturation","volume":"63","author":"Pra\u017cy\u0144ska","year":"2018","journal-title":"Folia Microbiol (Praha)"},{"key":"2020053120000836200_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1080\/08927014.2018.1472244","article-title":"Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition","volume":"34","author":"Rodrigues","year":"2018","journal-title":"Biofouling"},{"key":"2020053120000836200_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0132701","article-title":"Synergistic activity of the plant defensin HsAFP1 and caspofungin against Candida albicans biofilms and planktonic cultures","volume":"10","author":"Vriens","year":"2015","journal-title":"PLoS One"},{"key":"2020053120000836200_bib14","doi-asserted-by":"crossref","first-page":"685","DOI":"10.4014\/jmb.1607.07072","article-title":"Activity of allyl isothiocyanate and its synergy with fluconazole against Candida albicans biofilms","volume":"27","author":"Raut","year":"2017","journal-title":"J Microbiol Biotechnol"},{"key":"2020053120000836200_bib15","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1096\/fj.03-0168rev","article-title":"Resveratrol: from grapevines to mammalian biology","volume":"17","author":"Pervaiz","year":"2003","journal-title":"FASEB J"},{"key":"2020053120000836200_bib16","doi-asserted-by":"crossref","first-page":"306","DOI":"10.2337\/db07-0381","article-title":"Metformin inhibits hepatic gluconeogenesis through amp-activated protein kinase-dependent regulation of the orphan nuclear receptor sHP","volume":"57","author":"Kim","year":"2008","journal-title":"Diabetes"},{"key":"2020053120000836200_bib17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3892\/etm.2015.2895","article-title":"Bioavailability and safety study of resveratrol 500\u00a0mg tablets in healthy male and female volunteers","volume":"11","author":"Sergides","year":"2016","journal-title":"Exp Ther Med"},{"key":"2020053120000836200_bib18","first-page":"1","article-title":"Pterostilbene, a methoxylated resveratrol derivative, efficiently eradicates planktonic, biofilm, and intracellular MRSA by topical application","volume":"8","author":"Yang","year":"2017","journal-title":"Front Microbiol"},{"key":"2020053120000836200_bib19","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijantimicag.2018.03.002","article-title":"Can the quorum sensing inhibitor resveratrol function as an aminoglycoside antibiotic accelerant against Pseudomonas aeruginosa","volume":"52","author":"Zhou","year":"2018","journal-title":"Int J Antimicrob Agents"},{"key":"2020053120000836200_bib20","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s12223-017-0549-0","article-title":"Resveratrol, pterostilbene, and baicalein: plant-derived anti-biofilm agents","volume":"63","author":"Kolouchov\u00e1","year":"2018","journal-title":"Folia Microbiol (Praha)"},{"key":"2020053120000836200_bib21","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1080\/08927014.2014.976207","article-title":"Non-toxic plant metabolites regulate Staphylococcus viability and biofilm formation: a natural therapeutic strategy useful in the treatment and prevention of skin infections","volume":"30","author":"Mor\u00e1n","year":"2014","journal-title":"Biofouling"},{"key":"2020053120000836200_bib22","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1099\/00221287-143-2-303","article-title":"Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans","author":"Cormack","year":"1997","journal-title":"Microbiology"},{"key":"2020053120000836200_bib23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3390\/molecules22040651","article-title":"Antifungal and anti-biofilm activities of acetone lichen extracts against Candida albicans","volume":"22","author":"Millot","year":"2017","journal-title":"Molecules"},{"key":"2020053120000836200_bib24","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.ijantimicag.2010.12.016","article-title":"Possible role of azole and echinocandin lock solutions in the control of Candida biofilms associated with silicone","volume":"37","author":"Cateau","year":"2011","journal-title":"Int J Antimicrob Agents"},{"key":"2020053120000836200_bib25","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.algal.2018.11.015","article-title":"Spirulina platensis sustainable lipid extracts in alginate-based nanocarriers: an algal approach against biofilms","volume":"37","author":"Boutin","year":"2019","journal-title":"Algal Res"},{"key":"2020053120000836200_bib26","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1111\/1567-1364.12068","article-title":"Clinical isolates and laboratory reference Candida species and strains have varying abilities to form biofilms","volume":"13","author":"Alnuaimi","year":"2013","journal-title":"FEMS Yeast Res"},{"key":"2020053120000836200_bib27","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.ijantimicag.2008.01.028","article-title":"In vitro activity of terpenes against Candida biofilms","volume":"31","author":"Dalleau","year":"2008","journal-title":"Int J Antimicrob Agents"},{"key":"2020053120000836200_bib28","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1093\/jac\/dki269","article-title":"Anti-metabolic activity of caspofungin against Candida albicans and Candida parapsilosis biofilms","volume":"56","author":"Cocuaud","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"2020053120000836200_bib29","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1080\/08927014.2019.1608966","article-title":"Candida albicans enhances initial biofilm growth of Cutibacterium acnes under aerobic conditions","volume":"35","author":"Bernard","year":"2019","journal-title":"Biofouling"},{"key":"2020053120000836200_bib30","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/j.ijantimicag.2018.08.009","article-title":"Cutibacterium acnes protects Candida albicans from the effect of micafungin in biofilms","volume":"52","author":"Bernard","year":"2018","journal-title":"Int J Antimicrob Agents"},{"key":"2020053120000836200_bib31","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s12223-015-0400-4","article-title":"A flow cytometric approach to quantify biofilms","volume":"60","author":"Kerstens","year":"2015","journal-title":"Folia Microbiol (Praha)"},{"key":"2020053120000836200_bib32","first-page":"5","article-title":"Ex vivo red blood cell hemolysis assay for the evaluation of pH-responsive endosomolytic agents for cytosolic delivery of biomacromolecular drugs","author":"Evans","year":"2013","journal-title":"J Vis Exp"},{"key":"2020053120000836200_bib33","volume-title":"CompuSyn for Drug Combinations: PC Software and User's Guide: A Computer Program for Quantitation of Synergism and Antagonism in Drug Combinations, and the Determination of IC50 and ED50 and LD50 Values","author":"Chou","year":"2005"},{"key":"2020053120000836200_bib34","doi-asserted-by":"crossref","first-page":"495","DOI":"10.15698\/mic2018.11.656","article-title":"A chemical genetic screen reveals a role for proteostasis in capsule and biofilm formation by Cryptococcus neoformans","volume":"5","author":"Mayer","year":"2018","journal-title":"Microb Cell"},{"key":"2020053120000836200_bib35","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1128\/EC.00155-10","article-title":"Identification of a cell death pathway in Candida albicans during the response to pheromone","volume":"9","author":"Alby","year":"2010","journal-title":"Eukaryot Cell"},{"key":"2020053120000836200_bib36","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1371\/journal.ppat.1000040","article-title":"Stimulation of chitin synthesis rescues Candida albicans from echinocandins","volume":"4","author":"Walker","year":"2008","journal-title":"PLoS Pathog"}],"container-title":["Medical Mycology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/mmy\/article-pdf\/58\/4\/530\/33196561\/myz087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/mmy\/article-pdf\/58\/4\/530\/33196561\/myz087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:50Z","timestamp":1590969650000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mmy\/article\/58\/4\/530\/5556003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,28]]},"references-count":36,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,8,28]]},"published-print":{"date-parts":[[2020,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/mmy\/myz087","relation":{},"ISSN":["1369-3786","1460-2709"],"issn-type":[{"value":"1369-3786","type":"print"},{"value":"1460-2709","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,6]]},"published":{"date-parts":[[2019,8,28]]}}}