{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:50:22Z","timestamp":1790740222089,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,12,7]],"date-time":"2019-12-07T00:00:00Z","timestamp":1575676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Multiplex quantitative real-time PCR (MRT-PCR) using blood can improve the diagnosis of intra-abdominal candidiasis (IAC). We prospectively studied 39 patients with suspected IAC in the absence of previous antifungal therapy. Blood cultures, MRT-PCR, and \u03b2-D-glucan (BDG) in serum were performed in all patients. IAC was defined according to the 2013 European Consensus criteria. For MRT-PCR, the probes targeted the ITS1 or ITS2 regions of ribosomal DNA. Candidaemia was confirmed only in four patients (10%), and IAC criteria were present in 17 patients (43.6%). The sensitivity of MRT-PCR was 25% but increased to 63.6% (P\u00a0=\u00a0.06) in plasma obtained prior to volume overload and transfusion; specificity was above 85% in all cases. BDG performance was improved using a cutoff\u00a0&amp;gt;\u00a0260 pg\/ml, and improvement was not observed in samples obtained before transfusion. In this cohort of high risk of IAC and low rate of bloodstream infection, the performance of non-culture-based methods (MRT-PCR or BDG) was moderate but may be a complementary tool given the limitations of diagnostic methods available in clinical practice. Volume overload requirements, in combination with other factors, decrease the accuracy of MRT-PCR in patients with IAC.<\/jats:p>","DOI":"10.1093\/mmy\/myz123","type":"journal-article","created":{"date-parts":[[2019,11,23]],"date-time":"2019-11-23T20:09:23Z","timestamp":1574539763000},"page":"789-796","source":"Crossref","is-referenced-by-count":12,"title":["Roles of the multiplex real-time PCR assay and \u03b2-D-glucan in a high-risk population for intra-abdominal candidiasis (IAC)"],"prefix":"10.1093","volume":"58","author":[{"given":"J","family":"Fort\u00fan","sequence":"first","affiliation":[{"name":"Infectious Diseases Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Buitrago","sequence":"additional","affiliation":[{"name":"Reference Laboratory in Mycology; Centro Nacional Microbiolog\u00eda, Majadahonda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Gioia","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"G\u00f3mez-G\u00aa de la Pedrosa","sequence":"additional","affiliation":[{"name":"Microbiology Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M E","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Mart\u00edn-D\u00e1vila","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Pintado","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Cobeta","sequence":"additional","affiliation":[{"name":"Anaesthetic Department and Surgical Intensive Care Unit; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Martinez-Castro","sequence":"additional","affiliation":[{"name":"Anaesthetic Department and Surgical Intensive Care Unit; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Soriano","sequence":"additional","affiliation":[{"name":"Intensive Medicine Department and Medical Intensive Care Unit; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Moreno","sequence":"additional","affiliation":[{"name":"Surgey Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Corral","sequence":"additional","affiliation":[{"name":"Surgey Department; Hospital Ram\u00f3n y Cajal, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5706-5583","authenticated-orcid":false,"given":"P","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Department; Hospital Gregorio Mara\u00f1on, Madrid"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Moreno-Jimenez","sequence":"additional","affiliation":[{"name":"Hematology Department; Blood Bank Unit. 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