{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:26:02Z","timestamp":1740126362133,"version":"3.37.3"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T00:00:00Z","timestamp":1578441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100001597","name":"Willard L. 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We showed that CIP fungicidal activity, identical to the findings with cell culture medium, was inactivated by visible light and was unstable with storage in the dark. Congeners replacing either pyridoxal or L-cystine in CIP revealed structural requirements for fungicidal activity. Replacing pyridoxal in CIP with 2-hydroxy-5-nitrobenzaldehyde produced a solution that was equally fungicidal and maintained fungicidal activity upon storage in the dark for up to 50 days. We employed methods for excluding iron from CIP and found that fungicidal activity was not affected. Upon mixing L-cystine and pyridoxal in buffer at pH 7.0, diode array spectroscopy revealed a red-shift of absorbance maximum from 391\u00a0nm to 398\u00a0nm. Our findings point to Schiff base reaction between the pyridoxal aldehyde group of C1 with the alpha amino group(s) of cystine to yield a fungicidal compound. Light at wave length approximately 400\u00a0nm inactivates this complex accompanied by bleaching of the pyridine ring of pyridoxal. Our findings may be useful for design of a class of fungicidal compounds formed through Schiff base reaction of disulfide compounds with aromatic ring-bearing aldehydes.<\/jats:p>","DOI":"10.1093\/mmy\/myz130","type":"journal-article","created":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T12:11:20Z","timestamp":1576239080000},"page":"919-927","source":"Crossref","is-referenced-by-count":0,"title":["Properties of a fungicidal product formed from a reaction between L-cystine and pyridoxal"],"prefix":"10.1093","volume":"58","author":[{"given":"Elizabeth A","family":"Johnston","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spencer B","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald L","family":"Granger","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"2020101609550981300_bib1","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1093\/mmy\/myy040","article-title":"Combination of nutrients in a mammalian cell culture medium kills cryptococci","volume":"57","author":"Granger","year":"2019","journal-title":"Med Mycol"},{"key":"2020101609550981300_bib2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/BF02616112","article-title":"A survey of commercially available tissue culture media","volume":"6","author":"Morton","year":"1970","journal-title":"In 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