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To date, this cellular function is presumed to be restricted to mammals and birds, due to the absence of an identifiable lysosome-associated membrane protein 2A (LAMP2A), a limiting and essential protein for CMA, in nontetrapod species. However, the recent identification of expressed sequences displaying high homology with mammalian LAMP2A in several fish species challenges that view and suggests that CMA likely appeared earlier during evolution than initially thought. In the present study, we provide a comprehensive picture of the evolutionary history of the LAMP2 gene in vertebrates and demonstrate that LAMP2 indeed appeared at the root of the vertebrate lineage. Using a fibroblast cell line from medaka fish (Oryzias latipes), we further show that the splice variant lamp2a controls, upon long-term starvation, the lysosomal accumulation of a fluorescent reporter commonly used to track CMA in mammalian cells. Finally, to address the physiological role of Lamp2a in fish, we generated knockout medaka for that specific splice variant, and found that these deficient fish exhibit severe alterations in carbohydrate and fat metabolisms, in consistency with existing data in mice deficient for CMA in liver. Altogether, our data provide the first evidence for a CMA-like pathway in fish and bring new perspectives on the use of complementary genetic models, such as zebrafish or medaka, for studying CMA in an evolutionary perspective.<\/jats:p>","DOI":"10.1093\/molbev\/msaa127","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T07:10:11Z","timestamp":1589785811000},"page":"2887-2899","source":"Crossref","is-referenced-by-count":49,"title":["Chaperone-Mediated Autophagy in the Light of Evolution: Insight from Fish"],"prefix":"10.1093","volume":"37","author":[{"given":"Laury","family":"Lescat","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"V\u00e9ron","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Mourot","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"P\u00e9ron","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Chenais","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Dias","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e0lia","family":"Riera-Heredia","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Beaumatin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Pinel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Priault","sequence":"additional","affiliation":[{"name":"CNRS, IBGC, UMR5095, Bordeaux, France"},{"name":"IBGC, UMR5095, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Panserat","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Pau et des Pays de l\u2019Adour, E2S UPPA, INRAE, UMR1419 Nutrition M\u00e9tabolisme et Aquaculture, F-64310 Saint-P\u00e9e-sur-Nivelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Salin","sequence":"additional","affiliation":[{"name":"CNRS, IBGC, UMR5095, Bordeaux, France"},{"name":"IBGC, UMR5095, Universit\u00e9 de Bordeaux, Bordeaux, France"},{"name":"Service Commun de Microscopie, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Guiguen","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Bobe","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaury","family":"Herpin","sequence":"additional","affiliation":[{"name":"INRAE, UR1037 Laboratory of Fish Physiology and Genomics, Campus de Beaulieu, Rennes, France"},{"name":"State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, Hunan, P.R. 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