{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:09:43Z","timestamp":1787162983612,"version":"build-2736575974"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T00:00:00Z","timestamp":1606521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2017-03953"],"award-info":[{"award-number":["2017-03953"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002805","name":"Carl Trygger Foundation","doi-asserted-by":"publisher","award":["CTS17:204"],"award-info":[{"award-number":["CTS17:204"]}],"id":[{"id":"10.13039\/501100002805","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Integration of a conjugative plasmid into a bacterial chromosome can promote the transfer of chromosomal DNA to other bacteria. Intraspecies chromosomal conjugation is believed responsible for creating the global pathogens Klebsiella pneumoniae ST258 and Escherichia coli ST1193. Interspecies conjugation is also possible but little is known about the genetic architecture or fitness of such hybrids. To study this, we generated by conjugation 14 hybrids of E.\u00a0coli and Salmonella enterica. These species belong to different genera, diverged from a common ancestor &amp;gt;100 Ma, and share a conserved order of orthologous genes with \u223c15% nucleotide divergence. Genomic analysis revealed that all but one hybrid had acquired a contiguous segment of donor E.\u00a0coli DNA, replacing a homologous region of recipient Salmonella chromosome, and ranging in size from \u223c100 to &amp;gt;4,000\u2009kb. Recombination joints occurred in sequences with higher-than-average nucleotide identity. Most hybrid strains suffered a large reduction in growth rate, but the magnitude of this cost did not correlate with the length of foreign DNA. Compensatory evolution to ameliorate the cost of low-fitness hybrids pointed towards disruption of complex genetic networks as a cause. Most interestingly, 4 of the 14 hybrids, in which from 45% to 90% of the Salmonella chromosome was replaced with E.\u00a0coli DNA, showed no significant reduction in growth fitness. These data suggest that the barriers to creating high-fitness interspecies hybrids may be significantly lower than generally appreciated with implications for the creation of novel species.<\/jats:p>","DOI":"10.1093\/molbev\/msaa307","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T07:34:44Z","timestamp":1606289684000},"page":"1472-1481","source":"Crossref","is-referenced-by-count":12,"title":["Genetic Architecture and Fitness of Bacterial Interspecies Hybrids"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3033-8830","authenticated-orcid":false,"given":"Katrin","family":"Bartke","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linn\u00e9a","family":"Garoff","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala 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