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Traditionally, gene expression is modeled as a continuous trait, leaving qualitative transitions largely unexplored. In this paper, we detail the development of new Bayesian inference techniques to study the evolutionary turnover of organ-specific transcriptomes, which we define as instances where orthologous genes gain or lose expression in a particular organ. To test these techniques, we analyze the transcriptomes of 2 male reproductive organs, testes and accessory glands, across 11 species of the Drosophila melanogaster species group. We first discretize gene expression states by estimating the probability that each gene is expressed in each organ and species. We then define a phylogenetic model of correlated transcriptome evolution in 2 or more organs and fit it to the expression state data. Inferences under this model imply that many genes have gained and lost expression in each organ, and that the 2 organs experienced accelerated transcriptome turnover on different branches of the Drosophila phylogeny.<\/jats:p>","DOI":"10.1093\/molbev\/msaf106","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T08:13:57Z","timestamp":1747124037000},"source":"Crossref","is-referenced-by-count":2,"title":["Quantifying Transcriptome Turnover on Phylogenies by Modeling Gene Expression as a Binary Trait"],"prefix":"10.1093","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1825-4685","authenticated-orcid":false,"given":"Ammon","family":"Thompson","sequence":"first","affiliation":[{"name":"Department of Evolution and Ecology, University of California , Davis, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5031-4820","authenticated-orcid":false,"given":"Michael 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