{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:56:43Z","timestamp":1790603803326,"version":"4.1.0"},"reference-count":115,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"CNRS Mission pour les Initiatives Transverses et Interdisciplinaires"},{"name":"80|Prime and the Agence Nationale de la Recherche","award":["ANR-25-CE12-0652-01"],"award-info":[{"award-number":["ANR-25-CE12-0652-01"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dynamic patterns of gene gain and loss play a major role in the diversification of eukaryotes, reflecting adaptation to a broad range of ecological contexts. Reconstructing the evolutionary history of genes provides a powerful framework for understanding how functional innovation shapes life-history traits. Here, we report a comprehensive analysis of gene gain and loss across the fission yeast clade, whose evolutionary trajectory remains elusive. Reductive evolution of metabolic genes is a major contributor to species diversification, as observed in other fungal taxa. Notably, we uncovered an evolutionary scenario in which an ancestral gene duplication was followed by lineage-specific loss of one or the other paralog, except in Schizosaccharomyces pombe, which retained both. We demonstrate that these paralogs encode catalytically active invertases, named Inv1 and Inv2, with distinct enzymatic properties, localization, regulation, and physiological roles. Inv1 is a secreted enzyme subject to glucose-catabolite repression and is the sole invertase required for sucrose assimilation, resembling canonical yeast invertases. In contrast, Inv2 is intracellular, constitutively expressed, and required for inducing sexual differentiation in response to nutrient availability. Overall, these findings reveal an unexpected role for carbon metabolism in modulating the haploid-diploid cycle of fission yeasts, suggesting that diversification of core metabolic functions may contribute to adaptation to environments with distinct sugar compositions.<\/jats:p>","DOI":"10.1093\/molbev\/msag233","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:23:17Z","timestamp":1788780197000},"source":"Crossref","is-referenced-by-count":0,"title":["Evolutionary diversification of invertase paralogs couples carbon metabolism and sexual reproduction in fission yeasts"],"prefix":"10.1093","volume":"43","author":[{"given":"Ambre","family":"Noly","sequence":"first","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Faux","sequence":"additional","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Shaldaeva","sequence":"additional","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louison","family":"Bois-Naegelin","sequence":"additional","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5997-8722","authenticated-orcid":false,"given":"Philippe","family":"Fort","sequence":"additional","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1501-0423","authenticated-orcid":false,"given":"Dominique","family":"Helmlinger","sequence":"additional","affiliation":[{"name":"CRBM, University of Montpellier, CNRS , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026092809120467700_msag233-B1","first-page":"80","article-title":"Extraction and partial kinetic properties of invertase from Schizosaccharomyces pombe","volume":"4","author":"Aburigal","year":"2014","journal-title":"Int J Food Sci Nutr Eng"},{"key":"2026092809120467700_msag233-B2","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1128\/EC.00141-12","article-title":"The Schizosaccharomyces pombe inv1+ regulatory region is unusually large and contains redundant cis-acting elements that function in a SAGA- and Swi\/Snf-dependent fashion","volume":"11","author":"Ahn","year":"2012","journal-title":"Eukaryot Cell."},{"key":"2026092809120467700_msag233-B3","doi-asserted-by":"publisher","first-page":"2175","DOI":"10.1016\/j.cub.2023.04.046","article-title":"Optimization of energy production and central carbon metabolism in a non-respiring eukaryote","volume":"33","author":"Alam","year":"2023","journal-title":"Curr Biol."},{"key":"2026092809120467700_msag233-B4","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.gene.2011.05.024","article-title":"Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria","volume":"484","author":"Alm\u00e9ciga-D\u00edaz","year":"2011","journal-title":"Gene"},{"key":"2026092809120467700_msag233-B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159292","article-title":"Sensitive and quantitative three-color protein imaging in fission yeast using spectrally diverse, recoded fluorescent proteins with experimentally-characterized in vivo maturation kinetics","volume":"11","author":"Al-Sady","year":"2016","journal-title":"PLoS One"},{"key":"2026092809120467700_msag233-B6","doi-asserted-by":"publisher","first-page":"13930","DOI":"10.1074\/jbc.M109.095430","article-title":"Structural and kinetic analysis of Schwanniomyces occidentalis invertase reveals a new oligomerization pattern and the role of its supplementary domain in substrate binding","volume":"285","author":"\u00c1lvaro-Benito","year":"2010","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B7","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1002\/yea.1815","article-title":"Novel episomal vectors and a highly efficient transformation procedure for the fission yeast Schizosaccharomyces japonicus","volume":"27","author":"Aoki","year":"2010","journal-title":"Yeast (Chichester, England)"},{"key":"2026092809120467700_msag233-B8","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1002\/(SICI)1097-0061(199807)14:10&lt;943::AID-YEA292&gt;3.0.CO;2-Y","article-title":"Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe","volume":"14","author":"Bahler","year":"1998","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B9","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1111\/j.1574-6968.2000.tb09412.x","article-title":"Role for trehalase during germination of spores in the fission yeast Schizosaccharomyces pombe","volume":"193","author":"Beltran","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"2026092809120467700_msag233-B10","doi-asserted-by":"publisher","first-page":"3026","DOI":"10.5897\/AJMR2013.5684","article-title":"Schizosaccharomyces selective differential media","volume":"7","author":"Benito","year":"2013","journal-title":"Afr J Microbiol Res"},{"key":"2026092809120467700_msag233-B11","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1002\/bit.260130505","article-title":"Kinetic modeling of the hydrolysis of sucrose by invertase","volume":"13","author":"Bowski","year":"1971","journal-title":"Biotech Bioeng"},{"key":"2026092809120467700_msag233-B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/femsyr\/foz038","article-title":"Schizosaccharomyces osmophilus sp. nov., an osmophilic fission yeast occurring in bee bread of different solitary bee species","volume":"19","author":"Brysch-Herzberg","year":"2019","journal-title":"FEMS Yeast Res."},{"key":"2026092809120467700_msag233-B13","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1002\/yea.3857","article-title":"Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey","volume":"40","author":"Brysch-Herzberg","year":"2023","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B14","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1002\/yea.3922","article-title":"Reinstatement of the fission yeast species Schizosaccharomyces versatilis Wickerham et Duprat, a sibling species of Schizosaccharomyces japonicus","volume":"41","author":"Brysch-Herzberg","year":"2024","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B15","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1007\/s10482-022-01720-0","article-title":"Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats","volume":"115","author":"Brysch-Herzberg","year":"2022","journal-title":"Antonie van Leeuwenhoek"},{"key":"2026092809120467700_msag233-B16","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin Chem."},{"key":"2026092809120467700_msag233-B17","doi-asserted-by":"publisher","first-page":"4486","DOI":"10.1091\/mbc.e11-07-0608","article-title":"Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B","volume":"22","author":"Buttrick","year":"2011","journal-title":"Mol Biol Cell."},{"key":"2026092809120467700_msag233-B18","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.1128\/JB.180.5.1342-1345.1998","article-title":"Characterization of mutants devoid of neutral trehalase activity in the fission yeast Schizosaccharomyces pombe: partial protection from heat shock and high-salt stress","volume":"180","author":"Cansado","year":"1998","journal-title":"J Bacteriol."},{"key":"2026092809120467700_msag233-B19","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/0092-8674(82)90384-1","article-title":"Two differentially regulated mRNAs with different 5\u2032 ends encode secreted and intracellular forms of yeast invertase","volume":"28","author":"Carlson","year":"1982","journal-title":"Cell"},{"key":"2026092809120467700_msag233-B20","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1128\/mcb.3.3.439-447.1983","article-title":"The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence","volume":"3","author":"Carlson","year":"1983","journal-title":"Mol Cell Biol."},{"key":"2026092809120467700_msag233-B21","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyag001","article-title":"PomBase in 2026: expanding knowledge, modelling connections","volume":"232","author":"Carme","year":"2026","journal-title":"Genetics"},{"key":"2026092809120467700_msag233-B22","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1083\/jcb.201105074","article-title":"Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation","volume":"195","author":"Chen","year":"2011","journal-title":"J Cell Biol"},{"key":"2026092809120467700_msag233-B23","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25158296","article-title":"Frequent acquisition of glycoside hydrolase family 32 (GH32) genes from bacteria via horizontal gene transfer drives adaptation of invertebrates to diverse sources of food and living habitats","volume":"25","author":"Cheng","year":"2024","journal-title":"Int J Mol Sci."},{"key":"2026092809120467700_msag233-B24","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1038\/nrg2482","article-title":"Turning a hobby into a job: how duplicated genes find new functions","volume":"9","author":"Conant","year":"2008","journal-title":"Nat Rev Genet"},{"key":"2026092809120467700_msag233-B25","doi-asserted-by":"publisher","first-page":"30299","DOI":"10.1074\/jbc.271.48.30299","article-title":"Mechanism of polypeptide translocation into the endoplasmic reticulum","volume":"271","author":"Corsi","year":"1996","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B26","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1186\/1471-2148-10-210","article-title":"BMGE (block mapping and gathering with entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments","volume":"10","author":"Criscuolo","year":"2010","journal-title":"BMC Evol Biol"},{"key":"2026092809120467700_msag233-B27","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.1093\/molbev\/msab080","article-title":"Horizontal gene transfer and gene duplication of \u03b2-fructofuranosidase confer lepidopteran insects metabolic benefits","volume":"38","author":"Dai","year":"2021","journal-title":"Mol Biol Evol"},{"key":"2026092809120467700_msag233-B28","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1093\/molbev\/msw041","article-title":"Horizontal gene transfer from bacteria has enabled the plant-parasitic nematode globodera pallida to feed on host-derived sucrose","volume":"33","author":"Danchin","year":"2016","journal-title":"Mol Biol Evol"},{"key":"2026092809120467700_msag233-B29","doi-asserted-by":"publisher","first-page":"678","DOI":"10.3390\/microorganisms12040678","article-title":"Yeast diversity in honey and pollen samples from stingless bees in the State of Bahia, Brazil: use of the MALDI-TOF MS\/genbank proteomic technique","volume":"12","author":"Da Silva","year":"2024","journal-title":"Microorganisms"},{"key":"2026092809120467700_msag233-B30","doi-asserted-by":"publisher","first-page":"2663","DOI":"10.1038\/s41467-024-47017-w","article-title":"Macroevolutionary dynamics of gene family gain and loss along multicellular eukaryotic lineages","volume":"15","author":"Domazet-Lo\u0161o","year":"2024","journal-title":"Nat Commun"},{"key":"2026092809120467700_msag233-B31","doi-asserted-by":"publisher","first-page":"D571","DOI":"10.1093\/nar\/gkab1045","article-title":"The carbohydrate-active enzyme database: functions and literature","volume":"50","author":"Drula","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"2026092809120467700_msag233-B32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2532532123","article-title":"Gene loss, repression, amplification, and horizontal acquisition shape galactose\/melibiose metabolism in fission yeast","volume":"123","author":"Du","year":"2026","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026092809120467700_msag233-B33","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/BF00387025","article-title":"Physiological aspects of conjugation in fission yeast","volume":"98","author":"Egel","year":"1971","journal-title":"Planta"},{"key":"2026092809120467700_msag233-B34","doi-asserted-by":"publisher","first-page":"4387","DOI":"10.1016\/S0021-9258(18)61360-2","article-title":"Structure, assembly, and secretion of octameric invertase","volume":"262","author":"Esmon","year":"1987","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B35","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1002\/yea.3919","article-title":"Schizosaccharomyces versatilis represents a distinct evolutionary lineage of fission yeast","volume":"41","author":"Etherington","year":"2024","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104241","article-title":"Population genomics of the fission yeast Schizosaccharomyces pombe","volume":"9","author":"Fawcett","year":"2014","journal-title":"PLoS One"},{"key":"2026092809120467700_msag233-B37","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1002\/yea.1347","article-title":"Basic methods for fission yeast","volume":"23","author":"Forsburg","year":"2006","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B38","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1091\/mbc.e13-12-0731","article-title":"The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3","volume":"25","author":"Fukunishi","year":"2014","journal-title":"Mol Biol Cell."},{"key":"2026092809120467700_msag233-B39","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1016\/S0021-9258(18)93580-5","article-title":"Comparative study of the properties of the purified internal and external invertases from yeast","volume":"243","author":"Gasc\u00f3n","year":"1968","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B40","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00035-22","article-title":"Contrasting strategies for sucrose utilization in a floral yeast clade","volume":"7","author":"Gon\u00e7alves","year":"2022","journal-title":"mSphere"},{"key":"2026092809120467700_msag233-B41","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1002\/yea.3463","article-title":"The Wickerhamiella\/Starmerella clade\u2014a treasure trove for the study of the evolution of yeast metabolism","volume":"37","author":"Gon\u00e7alves","year":"2020","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B42","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1128\/EC.00361-06","article-title":"Molecular and biochemical characterization of a novel intracellular invertase from Aspergillus niger with transfructosylating activity","volume":"6","author":"Goosen","year":"2007","journal-title":"Eukaryot Cell."},{"key":"2026092809120467700_msag233-B43","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/nature07921","article-title":"Snowdrift game dynamics and facultative cheating in yeast","volume":"459","author":"Gore","year":"2009","journal-title":"Nature"},{"key":"2026092809120467700_msag233-B44","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1007\/s00239-021-10028-y","article-title":"Variables influencing differences in sequence conservation in the fission yeast Schizosaccharomyces pombe","volume":"89","author":"Harnqvist","year":"2021","journal-title":"J Mol Evol."},{"key":"2026092809120467700_msag233-B45","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.1093\/molbev\/msaa172","article-title":"Gene loss predictably drives evolutionary adaptation","volume":"37","author":"Helsen","year":"2020","journal-title":"Mol Biol Evol."},{"key":"2026092809120467700_msag233-B46","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1042\/bj2800309","article-title":"A classification of glycosyl hydrolases based on amino acid sequence similarities","volume":"280","author":"Henrissat","year":"1991","journal-title":"Biochem J."},{"key":"2026092809120467700_msag233-B47","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1002\/yea.1291","article-title":"Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe","volume":"22","author":"Hentges","year":"2005","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B48","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/ng.3215","article-title":"The genomic and phenotypic diversity of Schizosaccharomyces pombe","volume":"47","author":"Jeffares","year":"2015","journal-title":"Nat Genet."},{"key":"2026092809120467700_msag233-B49","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkad028","article-title":"A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus","volume":"13","author":"Jia","year":"2023","journal-title":"G3 (Bethesda, Md.)"},{"key":"2026092809120467700_msag233-B50","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/yea.70019","article-title":"Schizosaccharomyces orthogroup (SOG) resource: a web platform for exploring gene conservation in fission yeasts","volume":"43","author":"Jia","year":"2026","journal-title":"Yeast"},{"key":"2026092809120467700_msag233-B51","doi-asserted-by":"publisher","first-page":"8264","DOI":"10.1021\/bi201284u","article-title":"The original Michaelis constant: translation of the 1913 Michaelis\u2013Menten paper","volume":"50","author":"Johnson","year":"2011","journal-title":"Biochemistry"},{"key":"2026092809120467700_msag233-B52","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"2026092809120467700_msag233-B53","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1080\/09168451.2017.1401914","article-title":"Schizosaccharomyces japonicus has low levels of CoQ10 synthesis, respiration deficiency, and efficient ethanol production","volume":"82","author":"Kaino","year":"2018","journal-title":"Biosci Biotechnol Biochem."},{"key":"2026092809120467700_msag233-B54","doi-asserted-by":"publisher","first-page":"2929","DOI":"10.1242\/jcs.114.16.2929","article-title":"Intracellular pH homeostasis during cell-cycle progression and growth state transition in Schizosaccharomyces pombe","volume":"114","author":"Karagiannis","year":"2001","journal-title":"J Cell Sci."},{"key":"2026092809120467700_msag233-B55","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol Biol Evol"},{"key":"2026092809120467700_msag233-B56","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1093\/bbb\/zbae019","article-title":"Regulation of sexual differentiation initiation in Schizosaccharomyces pombe","volume":"88","author":"Kawamukai","year":"2024","journal-title":"Biosci Biotechnol Biochem."},{"key":"2026092809120467700_msag233-B57","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1111\/nph.19595","article-title":"Multiple horizontal gene transfer events have shaped plant glycosyl hydrolase diversity and function","volume":"242","author":"Kfoury","year":"2024","journal-title":"New Phytol."},{"key":"2026092809120467700_msag233-B58","doi-asserted-by":"publisher","first-page":"587","DOI":"10.18632\/aging.100682","article-title":"A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast","volume":"6","author":"Kim","year":"2014","journal-title":"Aging (Albany NY)."},{"key":"2026092809120467700_msag233-B59","doi-asserted-by":"publisher","first-page":"2197","DOI":"10.15252\/embr.201744942","article-title":"TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability","volume":"18","author":"Laboucari\u00e9","year":"2017","journal-title":"EMBO Rep."},{"key":"2026092809120467700_msag233-B60","first-page":"49","volume-title":"Ecological genomics. Vol. 781. Advances in experimental medicine and biology","author":"Leducq","year":"2014"},{"key":"2026092809120467700_msag233-B61","doi-asserted-by":"publisher","first-page":"W260","DOI":"10.1093\/nar\/gkz303","article-title":"NGPhylogeny.fr: new generation phylogenetic services for non-specialists","volume":"47","author":"Lemoine","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2026092809120467700_msag233-B62","doi-asserted-by":"publisher","first-page":"7810","DOI":"10.1038\/s41467-024-52043-9","article-title":"Experimental evolution of yeast shows that public-goods upregulation can evolve despite challenges from exploitative non-producers","volume":"15","author":"Lindsay","year":"2024","journal-title":"Nat Commun"},{"key":"2026092809120467700_msag233-B63","doi-asserted-by":"publisher","first-page":"320","DOI":"10.3390\/plants14030320","article-title":"Roles and regulations of acid invertases in plants: current knowledge and future perspectives","volume":"14","author":"Liu","year":"2025","journal-title":"Plants"},{"key":"2026092809120467700_msag233-B64","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.110011","article-title":"Evolution and functional roles of neutral\/alkaline invertases in plant growth, development, and stress response","volume":"225","author":"Liu","year":"2025","journal-title":"Plant Physiol Biochem"},{"key":"2026092809120467700_msag233-B65","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1080\/13873954.2023.2236681","article-title":"Generalized logistic model of bacterial growth","volume":"29","author":"Lo Grasso","year":"2023","journal-title":"Math Comput Modell Dyn Syst"},{"key":"2026092809120467700_msag233-B66","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.263404","article-title":"An improved tetracycline-inducible expression system for fission yeast","volume":"137","author":"Lyu","year":"2024","journal-title":"J Cell Sci."},{"key":"2026092809120467700_msag233-B67","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1186\/s13059-016-1101-2","article-title":"Functional and regulatory profiling of energy metabolism in fission yeast","volume":"17","author":"Malecki","year":"2016","journal-title":"Genome Biol."},{"key":"2026092809120467700_msag233-B68","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2020.101599","article-title":"A review on invertase: its potentials and applications","volume":"25","author":"Manoochehri","year":"2020","journal-title":"Biocatal Agric Biotechnol."},{"key":"2026092809120467700_msag233-B69","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nrg.2017.49","article-title":"Evolutionary biology through the lens of budding yeast comparative genomics","volume":"18","author":"Marsit","year":"2017","journal-title":"Nat Rev Genet"},{"key":"2026092809120467700_msag233-B70","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1242\/jcs.5.2.373","article-title":"Linear synthesis of sucrase and phosphatases during the cell cycle of Schizosaccharomyces pombe","volume":"5","author":"Mitchison","year":"1969","journal-title":"J Cell Sci."},{"key":"2026092809120467700_msag233-B71","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1007\/BF00491906","article-title":"Subcellular localization and glycoprotein nature of the invertase from the fission yeast Schizosaccharomyces pombe","volume":"142","author":"Moreno","year":"1985","journal-title":"Arch Microbiol"},{"key":"2026092809120467700_msag233-B72","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1042\/bj2670697","article-title":"Purification and characterization of the invertase from Schizosaccharomyces pombe. A comparative analysis with the invertase from Saccharomyces cerevisiae","volume":"267","author":"Moreno","year":"1990","journal-title":"Biochem J."},{"key":"2026092809120467700_msag233-B73","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1093\/genetics\/53.1.165","article-title":"Genetic mapping in Saccharomyces","volume":"53","author":"Mortimer","year":"1966","journal-title":"Genetics"},{"key":"2026092809120467700_msag233-B74","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.82595","article-title":"Host-microbiome metabolism of a plant toxin in bees","volume":"11","author":"Motta","year":"2022","journal-title":"eLife"},{"key":"2026092809120467700_msag233-B75","doi-asserted-by":"publisher","first-page":"297","DOI":"10.2323\/jgam.42.297","article-title":"Mechanism of sucrose utilisation by Saccharomyces cerevisiae","volume":"42","author":"Mwesigye","year":"1996","journal-title":"J Gen Appl Microbiol."},{"key":"2026092809120467700_msag233-B76","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1016\/j.procbio.2015.04.015","article-title":"Microbial invertases: a review on kinetics, thermodynamics, physiochemical properties","volume":"50","author":"Nadeem","year":"2015","journal-title":"Process Biochem"},{"key":"2026092809120467700_msag233-B77","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1021\/bi00854a015","article-title":"Purification and properties of yeast invertase","volume":"6","author":"Neumann","year":"1967","journal-title":"Biochemistry"},{"key":"2026092809120467700_msag233-B78","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/978-1-0716-4007-4_17","volume-title":"Protein bioinformatics","author":"Nielsen","year":"2024"},{"key":"2026092809120467700_msag233-B79","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1186\/s12862-016-0651-y","article-title":"Horizontal gene transfer of acetyltransferases, invertases and chorismate mutases from different bacteria to diverse recipients","volume":"16","author":"Noon","year":"2016","journal-title":"BMC Evol Biol"},{"key":"2026092809120467700_msag233-B80","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/s12915-018-0498-3","article-title":"Factors driving metabolic diversity in the budding yeast subphylum","volume":"16","author":"Opulente","year":"2018","journal-title":"BMC Biol"},{"key":"2026092809120467700_msag233-B81","doi-asserted-by":"publisher","DOI":"10.1126\/science.adj4503","article-title":"Genomic factors shape carbon and nitrogen metabolic niche breadth across Saccharomycotina yeasts","volume":"384","author":"Opulente","year":"2024","journal-title":"Science"},{"key":"2026092809120467700_msag233-B82","doi-asserted-by":"publisher","first-page":"2294","DOI":"10.1104\/pp.16.00523","article-title":"Evolution of gene duplication in plants","volume":"171","author":"Panchy","year":"2016","journal-title":"Plant Physiol"},{"key":"2026092809120467700_msag233-B83","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1186\/1471-2148-9-148","article-title":"Friend or foe? Evolutionary history of glycoside hydrolase family 32 genes encoding for sucrolytic activity in fungi and its implications for plant-fungal symbioses","volume":"9","author":"Parrent","year":"2009","journal-title":"BMC Evol Biol."},{"key":"2026092809120467700_msag233-B84","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/0092-8674(81)90071-4","article-title":"Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene","volume":"25","author":"Perlman","year":"1981","journal-title":"Cell"},{"key":"2026092809120467700_msag233-B85","doi-asserted-by":"publisher","first-page":"5033","DOI":"10.1073\/pnas.83.14.5033","article-title":"Mutations affecting the signal sequence alter synthesis and secretion of yeast invertase","volume":"83","author":"Perlman","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026092809120467700_msag233-B86","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1101\/gr.172098.114","article-title":"Genomic evidence for adaptation by gene duplication","volume":"24","author":"Qian","year":"2014","journal-title":"Genome Res"},{"key":"2026092809120467700_msag233-B87","doi-asserted-by":"publisher","first-page":"6978","DOI":"10.1016\/S0021-9258(18)68592-8","article-title":"Characterization of the glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons","volume":"263","author":"Reddy","year":"1988","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B88","doi-asserted-by":"publisher","first-page":"10817","DOI":"10.1016\/S0021-9258(19)38518-7","article-title":"Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis","volume":"265","author":"Reddy","year":"1990","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B89","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1046\/j.1365-2958.2001.02237.x","article-title":"Functional characterization of the \u03b1-glucoside transporter Sut1p from Schizosaccharomyces pombe, the first fungal homologue of plant sucrose transporters","volume":"39","author":"Reinders","year":"2001","journal-title":"Mol Microbiol."},{"key":"2026092809120467700_msag233-B90","doi-asserted-by":"publisher","first-page":"930","DOI":"10.1126\/science.1203357","article-title":"Comparative functional genomics of the fission yeasts","volume":"332","author":"Rhind","year":"2011","journal-title":"Science (New York, N.Y.)"},{"key":"2026092809120467700_msag233-B91","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1146\/annurev-arplant-050213-040251","article-title":"Sucrose metabolism: gateway to diverse carbon use and sugar signaling","volume":"65","author":"Ruan","year":"2014","journal-title":"Annu Rev Plant Biol."},{"key":"2026092809120467700_msag233-B92","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab223","article-title":"JaponicusDB: rapid deployment of a model organism database for an emerging model species","volume":"220","author":"Rutherford","year":"2022","journal-title":"Genetics"},{"key":"2026092809120467700_msag233-B93","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyae007","article-title":"PomBase: a global core biodata resource\u2014growth, collaboration, and sustainability","volume":"227","author":"Rutherford","year":"2024","journal-title":"Genetics"},{"key":"2026092809120467700_msag233-B94","doi-asserted-by":"publisher","first-page":"9755","DOI":"10.1074\/jbc.M112.446435","article-title":"Three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity","volume":"288","author":"Sainz-Polo","year":"2013","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B95","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.3390\/metabo3041118","article-title":"Metabolomic analysis of fission yeast at the onset of nitrogen starvation","volume":"3","author":"Sajiki","year":"2013","journal-title":"Metabolites"},{"key":"2026092809120467700_msag233-B96","doi-asserted-by":"publisher","first-page":"e0355697","DOI":"10.1371\/journal.pone.0355697","article-title":"Diversity of spore morphology among fission yeasts","volume":"21","author":"Sakaguchi","year":"2026","journal-title":"PLOS One"},{"key":"2026092809120467700_msag233-B97","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2405553121","article-title":"Cytoplasmic fluidization contributes to breaking spore dormancy in fission yeast","volume":"121","author":"Sakai","year":"2024","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026092809120467700_msag233-B98","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1101\/gr.6341207","article-title":"A burst of protein sequence evolution and a prolonged period of asymmetric evolution follow gene duplication in yeast","volume":"18","author":"Scannell","year":"2008","journal-title":"Genome Res"},{"key":"2026092809120467700_msag233-B99","doi-asserted-by":"publisher","first-page":"8827","DOI":"10.1016\/S0021-9258(18)68381-4","article-title":"The stability of yeast invertase is not significantly influenced by glycosylation","volume":"263","author":"Sch\u00fclke","year":"1988","journal-title":"J Biol Chem."},{"key":"2026092809120467700_msag233-B100","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fox045","article-title":"Mating response and construction of heterothallic strains of the fission yeast Schizosaccharomyces octosporus","volume":"17","author":"Seike","year":"2017","journal-title":"FEMS Yeast Res"},{"key":"2026092809120467700_msag233-B101","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1016\/j.cell.2018.10.023","article-title":"Tempo and mode of genome evolution in the budding yeast subphylum","volume":"175","author":"Shen","year":"2018","journal-title":"Cell"},{"key":"2026092809120467700_msag233-B102","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3390\/jof7010007","article-title":"Quick-freeze, deep-etch electron microscopy reveals the characteristic architecture of the fission yeast spore","volume":"7","author":"Tahara","year":"2020","journal-title":"J Fungi (Basel)"},{"key":"2026092809120467700_msag233-B103","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.mito.2015.04.003","article-title":"The critical glucose concentration for respiration-independent proliferation of fission yeast, Schizosaccharomyces pombe","volume":"22","author":"Takeda","year":"2015","journal-title":"Mitochondrion"},{"key":"2026092809120467700_msag233-B104","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1006\/bbrc.1998.8406","article-title":"Isolation and characterization of an invertase and its repressor genes from Schizosaccharomyces pombe","volume":"245","author":"Tanaka","year":"1998","journal-title":"Biochem Biophys Res Commun."},{"key":"2026092809120467700_msag233-B105","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyad031","article-title":"The Gene Ontology knowledgebase in 2023","volume":"224","author":"The Gene Ontology Consortium","year":"2023","journal-title":"Genetics"},{"key":"2026092809120467700_msag233-B106","doi-asserted-by":"publisher","first-page":"4782","DOI":"10.1111\/febs.13536","article-title":"Sequence and structure-based prediction of fructosyltransferase activity for functional subclassification of fungal GH32 enzymes","volume":"282","author":"Trollope","year":"2015","journal-title":"FEBS J."},{"key":"2026092809120467700_msag233-B107","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1186\/s12864-019-5602-8","article-title":"A genome-wide analysis of carbon catabolite repression in Schizosaccharomyces pombe","volume":"20","author":"Vassiliadis","year":"2019","journal-title":"BMC genomics"},{"key":"2026092809120467700_msag233-B108","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.240754","article-title":"A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe","volume":"133","author":"Vje\u0161tica","year":"2020","journal-title":"J Cell Sci."},{"key":"2026092809120467700_msag233-B109","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001067","article-title":"Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance","volume":"19","author":"Vje\u0161tica","year":"2021","journal-title":"PLoS Biol."},{"key":"2026092809120467700_msag233-B110","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.tplants.2017.11.001","article-title":"Evolution of sucrose metabolism: the dichotomy of invertases and beyond","volume":"23","author":"Wan","year":"2018","journal-title":"Trends Plant Sci."},{"key":"2026092809120467700_msag233-B111","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.biombioe.2012.05.003","article-title":"Rapid quantification of reducing sugars in biomass hydrolysates: improving the speed and precision of the dinitrosalicylic acid assay","volume":"44","author":"Wood","year":"2012","journal-title":"Biomass Bioenergy."},{"key":"2026092809120467700_msag233-B112","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s12915-022-01346-8","article-title":"Reconstruction of gene innovation associated with major evolutionary transitions in the kingdom fungi","volume":"20","author":"Wu","year":"2022","journal-title":"BMC Biol"},{"key":"2026092809120467700_msag233-B113","doi-asserted-by":"publisher","first-page":"D996","DOI":"10.1093\/nar\/gkab1007","article-title":"Ensembl genomes 2022: an expanding genome resource for non-vertebrates","volume":"50","author":"Yates","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2026092809120467700_msag233-B114","doi-asserted-by":"publisher","first-page":"2067","DOI":"10.1534\/g3.118.200164","article-title":"A cloning-free method for CRISPR\/Cas9-mediated genome editing in fission yeast","volume":"8","author":"Zhang","year":"2018","journal-title":"G3 (Bethesda, Md.)"},{"key":"2026092809120467700_msag233-B115","doi-asserted-by":"publisher","first-page":"6986","DOI":"10.1016\/S0021-9258(18)68593-X","article-title":"Characterization of the glycosylation sites in yeast external invertase. II. Location of the endo-beta-N-acetylglucosaminidase H-resistant sequons","volume":"263","author":"Ziegler","year":"1988","journal-title":"J Biol Chem."}],"container-title":["Molecular Biology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/mbe\/advance-article-pdf\/doi\/10.1093\/molbev\/msag233\/70996811\/msag233.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mbe\/article-pdf\/43\/9\/msag233\/70996811\/msag233.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/mbe\/article-pdf\/43\/9\/msag233\/70996811\/msag233.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:12:58Z","timestamp":1790601178000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mbe\/article\/doi\/10.1093\/molbev\/msag233\/8788012"}},"subtitle":[],"editor":[{"given":"Harmit","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":115,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/molbev\/msag233","relation":{},"ISSN":["0737-4038","1537-1719"],"issn-type":[{"value":"0737-4038","type":"print"},{"value":"1537-1719","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,9,1]]},"article-number":"msag233"}}