{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T17:09:50Z","timestamp":1775754590911,"version":"3.50.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Malaria remains a major public health challenge due to drug and insecticide resistance, underscoring the need for novel therapies with distinct mechanisms of action. In this study, silver nanoparticles were green-synthesized using the brown marine algae Padina tetrastromatica (Ag-PT) and evaluated through integrated in vitro, in vivo, metabolomics, network pharmacology, and in silico approaches. Ag-PT showed potent antiplasmodial activity, with significantly lower IC50 values and superior parasite suppression compared to chemically synthesized silver nanoparticles. Untargeted metabolomics revealed that Ag-PT treatment specifically restored malaria-induced disruptions in fatty acid, arginine, and arachidonic acid metabolism. This included elevating precursors of specialized pro-resolving mediators such as DHA, 14-HDHA, and 18-HEPE, and replenishing l-arginine to improve nitric oxide synthesis and vascular function. Integration with network pharmacology identified COX-2 (PTGS2) as a key hub gene. Molecular docking and dynamics confirmed strong binding of the Ag-PT phytochemical eriodictyol to COX-2, suggesting inhibition that shifts arachidonic acid metabolism toward anti-inflammatory specialized pro-resolving mediator production. Collectively, these findings reveal that Ag-PT offers a multifaceted therapeutic strategy by simultaneously targeting the parasite while modulating host inflammatory and metabolic pathways. This integrated therapeutic strategy highlights the potential of eco-friendly, plant-based nanomedicines as a next-generation intervention for malaria management.<\/jats:p>","DOI":"10.1093\/molecular-omics\/aaiag011","type":"journal-article","created":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:26:05Z","timestamp":1772861165000},"source":"Crossref","is-referenced-by-count":0,"title":["Green synthesis of silver nanoparticles using\n                    <i>Padina tetrastromatica<\/i>\n                    against malaria and deciphering the mechanism through machine learning-driven metabolomics and network pharmacology"],"prefix":"10.1093","volume":"22","author":[{"given":"Nikhila","family":"Yaladanda","sequence":"first","affiliation":[{"name":"Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka , Hyderabad 500007, Telangana ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yashwanth Kumar","family":"Budige","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka , Hyderabad 500007, Telangana ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dileepkumar","family":"Veeragoni","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka , Hyderabad 500007, Telangana ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhansi Venkata Nagamani","family":"Josyula","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka , Hyderabad 500007, Telangana ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Rodda","sequence":"additional","affiliation":[{"name":"Centre for Mass Spectrometry, Department of Analytical and Structural Chemistry, CSIR-Indian Institute of Chemical Technology , Hyderabad 500007 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0113-7394","authenticated-orcid":false,"given":"Vineeta","family":"Singh","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]},{"name":"Parasite and Host Biology Group, ICMR-National Institute of Malaria Research, Dwarka , New Delhi 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