{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:12:47Z","timestamp":1779120767114,"version":"3.51.4"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"NIAID Development of Sample Sparing Assays for Monitoring Immune Responses Infrastructure and Opportunity Fund","award":["NIH\/NIAID U24AI152179 to HS and KKS"],"award-info":[{"award-number":["NIH\/NIAID U24AI152179 to HS and KKS"]}]},{"name":"NIH\/NIAID Human Immunology Project Consortium","award":["U19AI089992 to HS"],"award-info":[{"award-number":["U19AI089992 to HS"]}]},{"name":"NIH\/NHLBI","award":["R35HL161175"],"award-info":[{"award-number":["R35HL161175"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Platelets are blood components not regularly analysed with proteomics due to the conventional wisdom that plasma for platelet research must be citrate-treated and freshly sampled to minimize artifactual changes. Information from platelets is complementary to plasma, specifically regarding immunophenotyping of patients with challenged immune systems due to infection or inflammation. We sought to develop a sample-sparing, high-throughput-compatible platelet proteomics workflow applicable to previously frozen, non-citrate platelet-rich plasma, in contrast to the field\u2019s standard, and test its efficacy by applying it to a COVID-19 cohort. We examined centrifugation of whole blood and platelet-rich plasma and volume requirements of plasma for platelet analysis. Platelet and platelet-poor plasma samples were analysed from a cohort of 79 patients, consisting of COVID-19 negative non-ICU and ICU controls and patients with COVID-19 over time. Conventional platelet count was successfully performed using flow cytometry on previously frozen plasma, showing minimal platelet aggregation and cell debris, demonstrating viability of previously frozen plasma for platelet proteomic analysis. Protein counts in platelets mostly mirrored trends in platelet count, except in severe COVID-19 patients within three days of admission to the ICU. Proteins dysregulated in this group compared to controls were enriched in terms related to platelet activation, phagosome, and efferocytosis. This agrees with prior reports using conventional platelet proteomics methods. Such similar findings suggest that the method developed here can utilize non-citrate, previously frozen plasma down to 0.5\u00a0\u03bcL per sample. This will make platelet proteomics studies on already collected, banked plasma samples more accessible and increase biomolecular information gained.<\/jats:p>","DOI":"10.1093\/molecular-omics\/aaiag017","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:04:15Z","timestamp":1777554255000},"source":"Crossref","is-referenced-by-count":0,"title":["Platelet proteomics on less than a drop of previously frozen, non-citrate plasma"],"prefix":"10.1093","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2151-3855","authenticated-orcid":false,"given":"Dylan Nicholas","family":"Tabang","sequence":"first","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Korff","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaitlin","family":"Brannon","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Tilburg","sequence":"additional","affiliation":[{"name":"Vascular Biology Program, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Komal","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Viod\u00e9","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick W","family":"van Zalm","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjun","family":"Ravishankar","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, Cornell University , New York, NY 10065 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Gyu","family":"Cheong","sequence":"additional","affiliation":[{"name":"Immunology and Microbial Pathogenesis Program and Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, Cornell University , New York, NY 10021 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Schott","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]},{"name":"Faculty of Process Sciences, Technical University Berlin , 10623 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6955-9310","authenticated-orcid":false,"given":"Ramy","family":"Arnaout","sequence":"additional","affiliation":[{"name":"Department of Pathology, Beth Israel Deaconess Medical Center , Boston, MA 02215 ,","place":["United States"]},{"name":"Division of Clinical Informatics, Department of Medicine, Beth Israel Deaconess Medical Center , Boston, MA 02215 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven Z","family":"Josefowicz","sequence":"additional","affiliation":[{"name":"Immunology and Microbial Pathogenesis Program and Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, Cornell University , New York, NY 10021 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph E","family":"Italiano","sequence":"additional","affiliation":[{"name":"Vascular Biology Program, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0179-6648","authenticated-orcid":false,"given":"Hanno","family":"Steen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02115,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8650-3693","authenticated-orcid":false,"given":"Kinga K","family":"Smolen","sequence":"additional","affiliation":[{"name":"Precision Vaccines Program, Department of Pediatrics, Boston Children\u2019s Hospital and Harvard Medical School , Boston, MA 02215 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"2026051811521389800_ref1","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.3389\/fimmu.2019.01320","article-title":"Regulation of innate immune responses by platelets","volume":"10","author":"Ribeiro","year":"2019","journal-title":"Front Immunol"},{"key":"2026051811521389800_ref2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232112772","article-title":"Platelets and their role in Hemostasis and thrombosis-from physiology to pathophysiology and 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