{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:05:44Z","timestamp":1790701544560,"version":"4.1.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100007036","name":"Danish Environmental Protection Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007036","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Health and Medical Research Council of Australia"},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP180103811"],"award-info":[{"award-number":["DP180103811"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>An adverse outcome pathway (AOP) is a simplified description of the sequence of mechanistic events that lead to a particular toxicological effect, from initial trigger to adverse outcome. Although designed to inform regulatory risk assessors, the AOP framework also provides a platform for innovative collaborations between experts from relevant research fields and the regulatory community. The underpinning for any AOP is basic knowledge about molecular and developmental processes; such knowledge can only be attained by solid bioscientific research. Starting with this fundamental knowledge, the objective is to devise novel testing strategies that focus on key events in a causative pathway. It is anticipated that such a knowledge-based approach will ultimately alleviate many of the burdens associated with classical chemical testing strategies that typically involve large-scale animal toxicity regimens. This hails from the notion that a solid understanding of the underlying mechanisms will allow the development and use of alternative test methods, including both in vitro and in silico approaches. This review is specifically targeted at professionals working in bioscientific fields, such as developmental and reproductive biology, and aims to (i) inform on the existence of the AOP framework and (ii) encourage new cross-disciplinary collaborations. It is hoped that fundamental biological knowledge can thus be better exploited for applied purposes: firstly, an improved understanding of how our perpetual exposure to environmental chemicals is causing human reproductive disease and, secondly, new approaches to screen for harmful chemicals more efficiently. This is not an instructional manual on how to create AOPs; rather, we discuss how to harness fundamental knowledge from the biosciences to assist regulatory toxicologists in their efforts to protect humans against chemicals that harm human reproductive development and function.<\/jats:p>","DOI":"10.1093\/molehr\/gaaa001","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:19:20Z","timestamp":1578064760000},"page":"111-116","source":"Crossref","is-referenced-by-count":17,"title":["Developmental biology meets toxicology: contributing reproductive mechanisms to build adverse outcome pathways"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6986-5991","authenticated-orcid":false,"given":"Monica Kam","family":"Draskau","sequence":"first","affiliation":[{"name":"Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassy M","family":"Spiller","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, The University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Boberg","sequence":"first","affiliation":[{"name":"Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Bowles","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, The University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4650-7651","authenticated-orcid":false,"given":"Terje","family":"Svingen","sequence":"first","affiliation":[{"name":"Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,16]]},"reference":[{"key":"2020022823095719800_ref1","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1002\/etc.34","article-title":"Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment","volume":"29","author":"Ankley","year":"2010","journal-title":"Environ Toxicol Chem"},{"key":"2020022823095719800_ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cotox.2018.03.004","article-title":"The adverse outcome pathway: a multifaceted framework supporting 21st century toxicology","volume":"9","author":"Ankley","year":"2018","journal-title":"Curr Opin Toxicol"},{"key":"2020022823095719800_ref3","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.devcel.2010.08.010","article-title":"FGF9 suppresses meiosis and promotes male germ cell fate in mice","volume":"19","author":"Bowles","year":"2010","journal-title":"Dev Cell"},{"key":"2020022823095719800_ref4","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1016\/j.celrep.2018.06.111","article-title":"Retinoic acid antagonizes testis development in mice","volume":"24","author":"Bowles","year":"2018","journal-title":"Cell Rep"},{"key":"2020022823095719800_ref5","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1002\/etc.4385","article-title":"Adverse outcome pathways: moving from a scientific concept to an internationally accepted framework","volume":"38","author":"Hecker","year":"2019","journal-title":"Environ Toxicol Chem"},{"key":"2020022823095719800_ref6","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1210\/endo-118-1-132","article-title":"Nipple differentiation in fetal male rats treated with an inhibitor of the enzyme 5 alpha-reductase: definition of a selective role for dihydrotestosterone","volume":"118","author":"Imperato-McGinley","year":"1986","journal-title":"Endocrinology"},{"key":"2020022823095719800_ref7","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1038\/nrendo.2017.36","article-title":"Environmental influences on ovarian dysgenesis - 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