{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T13:38:04Z","timestamp":1769348284110,"version":"3.49.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T00:00:00Z","timestamp":1584489600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401264; 81601308; 81701514; 81871135; 81971358"],"award-info":[{"award-number":["81401264; 81601308; 81701514; 81871135; 81971358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chinese Medical Association clinical doctors scientific research fund","award":["18010280757"],"award-info":[{"award-number":["18010280757"]}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LGF18H040005"],"award-info":[{"award-number":["LGF18H040005"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LY19H040013"],"award-info":[{"award-number":["LY19H040013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The study explores the role of neddylation in early trophoblast development and its alteration during the pathogenesis of recurrent spontaneous abortion (RSA). Immunofluorescence and western blot were conducted to evaluate the expression pattern of NEDD8 protein in the first-trimester placentas of healthy control and RSA patients. Neddylated-cullins, especially neddylated-cullin1, were downregulated and their substrate, p21, was accumulated in RSA samples. NEDD8 cytoplasmic recruitment was observed in extravillous trophoblast (EVT) progenitors of RSA placentas. Consistent with the results of clinical samples, neddylation inhibition using MLN4924 in trophoblast cell lines caused obvious p21 accumulation and free NEDD8 cytoplasmic recruitment. Further in vitro study demonstrated neddylation inhibition attenuated proliferation of Jeg-3 cells via p21 accumulation. Moreover, when trophoblast stem (TS) cells derived from first-trimester placentas were cultured for differentiation analyses. MLN4924 impaired the differentiation of TS cells towards EVTs by downregulating HLA-G and GATA3. p21 knockdown could partly rescue MLN4924-suppressed HLA-G and GATA3 expression. In conclusion, cullin1 neddylation-mediated p21 degradation is required for trophoblast proliferation and can affect trophoblast plasticity by affecting HLA-G and GATA3 expression. The results provide insights into the pathological mechanism of RSA and the biological regulation of trophoblast development.<\/jats:p>","DOI":"10.1093\/molehr\/gaaa021","type":"journal-article","created":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T12:30:53Z","timestamp":1583843453000},"source":"Crossref","is-referenced-by-count":9,"title":["Abnormal Cullin1 neddylation-mediated p21 accumulation participates in the pathogenesis of recurrent spontaneous abortion by regulating trophoblast cell proliferation and differentiation"],"prefix":"10.1093","author":[{"given":"Xiaohe","family":"Sun","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Tong","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqing","family":"Hao","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Li","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinli","family":"Zhang","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibin","family":"Pan","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongdong","family":"Dai","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Liu","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai","family":"Zhang","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8615-7196","authenticated-orcid":false,"given":"Songying","family":"Zhang","sequence":"first","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,3,18]]},"reference":[{"key":"2020050503074450500_ref1","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.molmed.2007.05.005","article-title":"Recurrent pregnancy loss: the key potential mechanisms","volume":"13","author":"Baek","year":"2007","journal-title":"Trends Mol Med"},{"key":"2020050503074450500_ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biocel.2004.05.014","article-title":"The human cytotrophoblastic cell, a mononuclear chameleon","volume":"37","author":"Bischof","year":"2005","journal-title":"Int J Biochem Cell Biol"},{"key":"2020050503074450500_ref3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.molcel.2009.12.024","article-title":"Substrate-assisted inhibition of ubiquitin-like protein-activating enzymes: the NEDD8 E1 inhibitor MLN4924 forms a NEDD8-AMP mimetic in situ","volume":"37","author":"Brownell","year":"2010","journal-title":"Mol Cell"},{"key":"2020050503074450500_ref4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1097\/01.gco.0000194112.86051.26","article-title":"Recurrent miscarriage: pathophysiology and outcome","volume":"17","author":"Carrington","year":"2005","journal-title":"Curr Opin Obstet Gynecol"},{"key":"2020050503074450500_ref5","doi-asserted-by":"crossref","first-page":"4145","DOI":"10.1158\/1078-0432.CCR-15-2254","article-title":"Neddylation inhibition activates the extrinsic apoptosis pathway through ATF4-CHOP-DR5 Axis in human esophageal cancer cells","volume":"22","author":"Chen","year":"2016","journal-title":"Clin Cancer Res"},{"key":"2020050503074450500_ref6","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/mrd.10331","article-title":"Expression of angiogenesis- and apoptosis-related genes in chorionic villi derived from recurrent pregnancy loss patients","volume":"66","author":"Choi","year":"2003","journal-title":"Mol Reprod Dev"},{"key":"2020050503074450500_ref7","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1006\/cimm.1994.1125","article-title":"Resistance of HLA-G and HLA-A2 transfectants to lysis by decidual NK cells","volume":"155","author":"Chumbley","year":"1994","journal-title":"Cell Immunol"},{"key":"2020050503074450500_ref8","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.aanat.2015.10.003","article-title":"Intermediate trophoblast--a distinctive, unique and often unrecognized population of trophoblastic cells","volume":"204","author":"Cierna","year":"2016","journal-title":"Ann Anat"},{"key":"2020050503074450500_ref9","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.cell.2008.07.022","article-title":"Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation","volume":"134","author":"Duda","year":"2008","journal-title":"Cell"},{"key":"2020050503074450500_ref10","doi-asserted-by":"crossref","first-page":"331","DOI":"10.2147\/IJWH.S100817","article-title":"Recurrent pregnancy loss: current perspectives","volume":"9","author":"El Hachem","year":"2017","journal-title":"Int J Womens Health"},{"key":"2020050503074450500_ref11","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1007\/s10815-017-0994-3","article-title":"The HLA-G 14-bp polymorphism and recurrent implantation failure: a meta-analysis","volume":"34","author":"Fan","year":"2017","journal-title":"J Assist Reprod Genet"},{"key":"2020050503074450500_ref12","doi-asserted-by":"crossref","first-page":"5364","DOI":"10.1073\/pnas.1602886113","article-title":"A distant trophoblast-specific enhancer controls HLA-G expression at the maternal-fetal interface","volume":"113","author":"Ferreira","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020050503074450500_ref13","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.it.2017.01.009","article-title":"HLA-G: at the interface of maternal-fetal tolerance","volume":"38","author":"Ferreira","year":"2017","journal-title":"Trends Immunol"},{"key":"2020050503074450500_ref14","doi-asserted-by":"crossref","first-page":"10870","DOI":"10.1074\/jbc.M109.004200","article-title":"Ubiquitin ligase cullin 7 induces epithelial-mesenchymal transition in human choriocarcinoma cells","volume":"285","author":"Fu","year":"2010","journal-title":"J Biol Chem"},{"key":"2020050503074450500_ref15","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1136\/postgradmedj-2014-132672","article-title":"Recurrent miscarriage: causes, evaluation and management","volume":"91","author":"Garrido-Gimenez","year":"2015","journal-title":"Postgrad Med J"},{"key":"2020050503074450500_ref16","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/s10815-016-0720-6","article-title":"Immunogenetic contributions to recurrent pregnancy loss","volume":"33","author":"Grimstad","year":"2016","journal-title":"J Assist Reprod Genet"},{"key":"2020050503074450500_ref17","doi-asserted-by":"crossref","first-page":"E7710","DOI":"10.1073\/pnas.1612335113","article-title":"Notch1 controls development of the extravillous trophoblast lineage in the human placenta","volume":"113","author":"Haider","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020050503074450500_ref18","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1242\/dev.145318","article-title":"Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development","volume":"144","author":"Home","year":"2017","journal-title":"Development"},{"key":"2020050503074450500_ref19","doi-asserted-by":"crossref","first-page":"28729","DOI":"10.1074\/jbc.M109.016840","article-title":"GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression","volume":"284","author":"Home","year":"2009","journal-title":"J Biol Chem"},{"key":"2020050503074450500_ref20","doi-asserted-by":"crossref","first-page":"6829","DOI":"10.1038\/sj.onc.1203093","article-title":"Covalent modification of all members of human cullin family proteins by NEDD8","volume":"18","author":"Hori","year":"1999","journal-title":"Oncogene"},{"key":"2020050503074450500_ref21","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.placenta.2012.01.020","article-title":"Human placentation from nidation to 5 weeks of gestation. Part I: what do we know about formative placental development following implantation?","volume":"33","author":"James","year":"2012","journal-title":"Placenta"},{"key":"2020050503074450500_ref22","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/j.humimm.2012.05.009","article-title":"HLA-G polymorphisms and soluble HLA-G protein levels in women with recurrent pregnancy loss from Basrah province in Iraq","volume":"73","author":"Jassem","year":"2012","journal-title":"Hum Immunol"},{"key":"2020050503074450500_ref23","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1593\/neo.11420","article-title":"Induction of p21-dependent senescence by an NAE inhibitor, MLN4924, as a mechanism of growth suppression","volume":"13","author":"Jia","year":"2011","journal-title":"Neoplasia"},{"key":"2020050503074450500_ref24","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1387\/ijdb.082769mk","article-title":"Critical growth factors and signalling pathways controlling human trophoblast invasion","volume":"54","author":"Knofler","year":"2010","journal-title":"Int J Dev Biol"},{"key":"2020050503074450500_ref25","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3389\/fgene.2013.00190","article-title":"Human placental trophoblast invasion and differentiation: a particular focus on Wnt signaling","volume":"4","author":"Knofler","year":"2013","journal-title":"Front Genet"},{"key":"2020050503074450500_ref26","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1126\/science.2326636","article-title":"A class I antigen, HLA-G, expressed in human trophoblasts","volume":"248","author":"Kovats","year":"1990","journal-title":"Science"},{"key":"2020050503074450500_ref27","doi-asserted-by":"publisher","DOI":"10.1038\/srep24218","article-title":"Neddylation inhibitor MLN4924 suppresses growth and migration of human gastric cancer cells","volume":"6","author":"Lan","year":"2016","journal-title":"Sci Rep"},{"key":"2020050503074450500_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dju083","article-title":"Overactivated neddylation pathway as a therapeutic target in lung cancer","volume":"106","author":"Li","year":"2014","journal-title":"J Natl Cancer Inst"},{"key":"2020050503074450500_ref29","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13144","article-title":"Downregulation of CCNA2 disturbs trophoblast migration, proliferation, and apoptosis during the pathogenesis of recurrent miscarriage","volume":"82","author":"Li","year":"2019","journal-title":"Am J Reprod Immunol"},{"key":"2020050503074450500_ref30","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1093\/humrep\/dev117","article-title":"NEDD8-mediated neddylation is required for human endometrial stromal proliferation and decidualization","volume":"30","author":"Liao","year":"2015","journal-title":"Hum Reprod"},{"key":"2020050503074450500_ref31","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1016\/j.placenta.2013.07.070","article-title":"HLA-G regulates the invasive properties of JEG-3 choriocarcinoma cells by controlling STAT3 activation","volume":"34","author":"Liu","year":"2013","journal-title":"Placenta"},{"key":"2020050503074450500_ref32","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/s41422-018-0066-y","article-title":"Single-cell RNA-seq reveals the diversity of trophoblast subtypes and patterns of differentiation in the human placenta","volume":"28","author":"Liu","year":"2018","journal-title":"Cell Res"},{"key":"2020050503074450500_ref33","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.omtn.2018.12.011","article-title":"The attenuation of trophoblast invasion caused by the downregulation of EZH2 is involved in the pathogenesis of human recurrent miscarriage","volume":"14","author":"Lv","year":"2019","journal-title":"Mol Ther Nucleic Acids"},{"key":"2020050503074450500_ref34","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1038\/nri1897","article-title":"Immunology of placentation in eutherian mammals","volume":"6","author":"Moffett","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"2020050503074450500_ref35","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.devcel.2009.02.003","article-title":"The hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass","volume":"16","author":"Nishioka","year":"2009","journal-title":"Dev Cell"},{"key":"2020050503074450500_ref36","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.stem.2017.11.004","article-title":"Derivation of human trophoblast stem cells","volume":"22","author":"Okae","year":"2018","journal-title":"Cell Stem Cell"},{"key":"2020050503074450500_ref37","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1038\/sj.onc.1207414","article-title":"Nedd8 on cullin: building an expressway to protein destruction","volume":"23","author":"Pan","year":"2004","journal-title":"Oncogene"},{"key":"2020050503074450500_ref38","doi-asserted-by":"crossref","first-page":"S61","DOI":"10.1016\/j.placenta.2017.05.005","article-title":"GATA factors: master regulators of gene expression in trophoblast progenitors","volume":"60","author":"Paul","year":"2017","journal-title":"Placenta"},{"key":"2020050503074450500_ref39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/nrm1547","article-title":"Function and regulation of cullin-RING ubiquitin ligases","volume":"6","author":"Petroski","year":"2005","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2020050503074450500_ref40","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/S0198-8859(00)00123-3","article-title":"Soluble HLA levels in early pregnancy after in vitro fertilization","volume":"61","author":"Pfeiffer","year":"2000","journal-title":"Hum Immunol"},{"key":"2020050503074450500_ref41","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.fertnstert.2012.09.023","article-title":"Definitions of infertility and recurrent pregnancy loss: a committee opinion","volume":"99","author":"Practice Committee of American Society for Reproductive M","year":"2013","journal-title":"Fertil Steril"},{"key":"2020050503074450500_ref42","first-page":"96","article-title":"Study on the expression of transcription factor GATA-3 and T-bet mRNA in decidua of women with unexplained recurrent spontaneous abortion","volume":"42","author":"Qiu","year":"2007","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"2020050503074450500_ref43","doi-asserted-by":"crossref","first-page":"20596","DOI":"10.1073\/pnas.1208248109","article-title":"Cellular senescence induced by CD158d reprograms natural killer cells to promote vascular remodeling","volume":"109","author":"Rajagopalan","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020050503074450500_ref44","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1172\/JCI200422991","article-title":"Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface","volume":"114","author":"Red-Horse","year":"2004","journal-title":"J Clin Invest"},{"key":"2020050503074450500_ref45","doi-asserted-by":"crossref","first-page":"11520","DOI":"10.1073\/pnas.94.21.11520","article-title":"Direct evidence to support the role of HLA-G in protecting the fetus from maternal uterine natural killer cytolysis","volume":"94","author":"Rouas-Freiss","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020050503074450500_ref46","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nature17408","article-title":"Derivation and differentiation of haploid human embryonic stem cells","volume":"532","author":"Sagi","year":"2016","journal-title":"Nature"},{"key":"2020050503074450500_ref47","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/gb-2011-12-4-220","article-title":"The cullin protein family","volume":"12","author":"Sarikas","year":"2011","journal-title":"Genome Biol"},{"key":"2020050503074450500_ref48","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/nature07884","article-title":"An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer","volume":"458","author":"Soucy","year":"2009","journal-title":"Nature"},{"key":"2020050503074450500_ref49","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1477-7827-3-56","article-title":"Human trophoblast function during the implantation process","volume":"3","author":"Staun-Ram","year":"2005","journal-title":"Reprod Biol Endocrinol"},{"key":"2020050503074450500_ref50","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/path.4694","article-title":"The YY1\/MMP2 axis promotes trophoblast invasion at the maternal-fetal interface","volume":"239","author":"Tian","year":"2016","journal-title":"J Pathol"},{"key":"2020050503074450500_ref51","doi-asserted-by":"crossref","first-page":"7219","DOI":"10.1073\/pnas.1507977112","article-title":"Human HLA-G+ extravillous trophoblasts: immune-activating cells that interact with decidual leukocytes","volume":"112","author":"Tilburgs","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020050503074450500_ref52","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1080\/19336918.2015.1089376","article-title":"Function and control of human invasive trophoblast subtypes: intrinsic vs. maternal control","volume":"10","author":"Velicky","year":"2016","journal-title":"Cell Adh Migr"},{"key":"2020050503074450500_ref53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007698","article-title":"Genome amplification and cellular senescence are hallmarks of human placenta development","volume":"14","author":"Velicky","year":"2018","journal-title":"PLoS Genet"},{"key":"2020050503074450500_ref54","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/tan.12056","article-title":"Association of 14-bp insertion\/deletion polymorphism of HLA-G gene with unexplained recurrent spontaneous abortion: a meta-analysis","volume":"81","author":"Wang","year":"2013","journal-title":"Tissue Antigens"},{"key":"2020050503074450500_ref55","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.301","article-title":"Role of peroxiredoxin2 downregulation in recurrent miscarriage through regulation of trophoblast proliferation and apoptosis","volume":"8","author":"Wu","year":"2017","journal-title":"Cell Death Dis"},{"key":"2020050503074450500_ref56","doi-asserted-by":"crossref","first-page":"4010","DOI":"10.1242\/dev.122846","article-title":"Human stem cells from single blastomeres reveal pathways of embryonic or trophoblast fate specification","volume":"142","author":"Zdravkovic","year":"2015","journal-title":"Development"},{"key":"2020050503074450500_ref57","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.1","article-title":"CUL1 promotes trophoblast cell invasion at the maternal-fetal interface","volume":"4","author":"Zhang","year":"2013","journal-title":"Cell Death Dis"},{"key":"2020050503074450500_ref58","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1530\/REP-15-0126","article-title":"New insights into the function of Cullin 3 in trophoblast invasion and migration","volume":"150","author":"Zhang","year":"2015","journal-title":"Reproduction"},{"key":"2020050503074450500_ref59","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12536","article-title":"The Nedd8-activating enzyme inhibitor MLN4924 (TAK-924\/Pevonedistat) induces apoptosis via c-Myc-Noxa axis in head and neck squamous cell carcinoma","volume":"52","author":"Zhang","year":"2019","journal-title":"Cell Prolif"},{"key":"2020050503074450500_ref60","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1089\/ars.2013.5795","article-title":"Targeting Neddylation pathways to inactivate cullin-RING ligases for anticancer therapy","volume":"21","author":"Zhao","year":"2014","journal-title":"Antioxid Redox Signal"},{"key":"2020050503074450500_ref61","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.2174\/13816128113199990300","article-title":"Cullin-RING ligases as attractive anti-cancer targets","volume":"19","author":"Zhao","year":"2013","journal-title":"Curr Pharm Des"},{"key":"2020050503074450500_ref62","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s12943-019-0979-1","article-title":"Neddylation: a novel modulator of the tumor microenvironment","volume":"18","author":"Zhou","year":"2019","journal-title":"Mol Cancer"},{"key":"2020050503074450500_ref63","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.cellsig.2018.01.009","article-title":"Protein neddylation and its alterations in human cancers for targeted therapy","volume":"44","author":"Zhou","year":"2018","journal-title":"Cell Signal"}],"container-title":["Molecular Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaaa021\/33165689\/gaaa021.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaaa021\/33116385\/gaaa021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaaa021\/33165689\/gaaa021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T07:08:49Z","timestamp":1588662529000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molehr\/advance-article\/doi\/10.1093\/molehr\/gaaa021\/5809512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,18]]},"references-count":63,"URL":"https:\/\/doi.org\/10.1093\/molehr\/gaaa021","relation":{},"ISSN":["1460-2407"],"issn-type":[{"value":"1460-2407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,18]]},"article-number":"gaaa021"}}