{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:07:08Z","timestamp":1792786028151,"version":"4.5.0"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T00:00:00Z","timestamp":1587686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Andalusian Government"},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["BIO-109"],"award-info":[{"award-number":["BIO-109"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["P11-CVI-7291"],"award-info":[{"award-number":["P11-CVI-7291"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["CGL2015-67108-P"],"award-info":[{"award-number":["CGL2015-67108-P"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The identification of new genes involved in sexual development and gonadal function as potential candidates causing male infertility is important for both diagnostic and therapeutic purposes. Deficiency of the onco-miRNA cluster miR-17\u223c92 has been shown to disrupt spermatogenesis, whereas mutations in its paralog cluster, miR-106b\u223c25, that is expressed in the same cells, were reported to have no effect on testis development and function. The aim of this work is to determine the role of these two miRNA clusters in spermatogenesis and male fertility. For this, we analyzed miR-106b\u223c25 and miR-17\u223c92 single and double mouse mutants and compared them to control mice. We found that miR-106b\u223c25 knock out testes show reduced size, oligozoospermia and altered spermatogenesis. Transcriptomic analysis showed that multiple molecular pathways are deregulated in these mutant testes. Nevertheless, mutant males conserved normal fertility even when early spermatogenesis and other functions were disrupted. In contrast, miR-17\u223c92+\/\u2212;\u2009miR-106b\u223c25\u2212\/\u2212 double mutants showed severely disrupted testicular histology and significantly reduced fertility. Our results indicate that miR-106b\u223c25 and miR-17\u223c92 ensure accurate gene expression levels in the adult testis, keeping them within the required thresholds. They play a crucial role in testis homeostasis and are required to maintain male fertility. Hence, we have identified new candidate genetic factors to be screened in the molecular diagnosis of human males with reproductive disorders. Finally, considering the well-known oncogenic nature of these two clusters and the fact that patients with reduced fertility are more prone to testicular cancer, our results might also help to elucidate the molecular mechanisms linking both pathologies.<\/jats:p>","DOI":"10.1093\/molehr\/gaaa027","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T15:16:48Z","timestamp":1586963808000},"page":"389-401","source":"Crossref","is-referenced-by-count":19,"title":["Deficiency of the onco-miRNA cluster, miR-106b\u223c25, causes oligozoospermia and the cooperative action of miR-106b\u223c25 and miR-17\u223c92 is required to maintain male fertility"],"prefix":"10.1093","volume":"26","author":[{"given":"Alicia","family":"Hurtado","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogelio","family":"Palomino","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular I e Instituto de Investigaci\u00f3n Biosanitaria de Granada, Universidad de Granada, Laboratorio 127 Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Georg","sequence":"first","affiliation":[{"name":"Genetics of Complex Diseases Unit, Pfizer-University of Granada-Junta de Andaluc\u00eda \u201cCentre for Genomics and Oncological Research\u201d (GENYO), Avenida de la Ilustraci\u00f3n 114, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Lao","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca M","family":"Real","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1427-7639","authenticated-orcid":false,"given":"F David","family":"Carmona","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Burgos","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4103-8219","authenticated-orcid":false,"given":"Rafael","family":"Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J","family":"Barrionuevo","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Instituto de Biotecnolog\u00eda, Universidad de Granada, Labs 127 and 105a, Centro de Investigaci\u00f3n Biom\u00e9dica, Avenida del Conocimiento, 18016 Armilla, Granada, 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